생명과학회지 (Journal of Life Science)

  • 제18권9호
  • /
  • Pages.1271-1277
  • /
  • 2008
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

건조방법에 따른 건조 양파 추출물의 항암 및 항산화 효과

Anticancer and Antioxidant Effects of Solvent Extracts from Dried Onion with Different Drying Methods

  • 장주리 (한국해양대학교 해양환경생명과학부) ;
  • 김경근 (한국해양대학교 기관시스템공학부) ;
  • 임선영 (한국해양대학교 해양환경생명과학부)
  • Jang, Joo-Ri (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Kim, Kyun-Kun (Division of Marine System Engineering, Korea Maritime University) ;
  • Lim, Sun-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
  • 발행 : 2008.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 동결건조 방법과 저온 진공 건조기를 도입하여 양파를 건조한 후 유기 용매로 추출하여 인체 결장암 및 섬유육종세포에 대한 증식 억제 효과와 세포 내 활성산소종 억제 효과에 대해 비교 검토하였다. 저온 진공 건조방법으로 건조된 양파 분말의 A+M 추출물은 가장 낮은 농도인 0.5 mg/ml에서부터 농도 의존적으로 인체 섬유육종 및 결장암세포의 성장을 억제시켜 동결 건조된 양파 A+M 추출물보다 높은 증식 억제효과를 보였고(p<0.05), A+M 추출물과 비교했을 때 낮은 증식 억제효과를 나타내었으나 두 가지 건조방법에 따른 각각의 양파 MeOH 추출물도 암세포 증식 억제효과를 보였다(p<0.05). 동결건조 및 저온 진공 건조방법에 따른 양파 분획물들도 높은 암세포 증식 억제효과를 나타내었으며 그 중에서 동결 건조된 양파의 water 분획물과 hexane 분획물은 저온 진공 건조된 양파의 경우 보다 다소 높은 저해효과를 나타내었다(p<0.05). 따라서 A+M 추출물과 MeOH 추출물의 경우에는 저온 진공건조에 의해 건조된 양파 추출물에 의한 암세포 증식 억제 효과가 컸으며 분획물들의 경우에는 동결 건조된 양파 추출물의 억제 효과가 다소 높았다. 지질과산화물 생성 억제효과 실험에서는 두 가지 건조 방법에 의해 건조된 양파 분말의 A+M 및 MeOH 추출물들과 그 분획물들은 첨가농도 2.5 mg/ml에서 blank군과 control군과 비교하였을 때 측정 시간 120분 동안 계속적으로 높은 세포 내 지질 과산화물 생성 억제 능력을 나타내었다(p<0.05). 세포내 활성산소종 억제효과는 동결건조에 의해 건조된 양파보다 저온 진공 건조방법에 의해 건조된 양파 분말의 추출물과 분획물들에 의한 저해효과가 더 높았다.

We investigated to determine the inhibitory effects of solvent extracts from dried onion on growth of cancer cell lines (HT-1080 human fibrosarcoma and HT-29 human colon cancer cells) and $H_{2}O_{2}$-induced oxidative stress. Two different drying methods, low temperature vacuum dryer and freeze dryer, were employed to dry onion. Inhibitory effects of acetone with methylene chloride (A+M) and methanol (MeOH) extracts from onion by two drying methods on the growth of HT-1080 and HT-29 cancer cells increased in a dose dependent manner (p<0.05) and the higher inhibitory effect was shown in onion extracts dried by low temperature vacuum dryer. The treatments of hexane, 85% aq. methanol, butanol and water fractions significantly inhibited the growth of both cancer cells (p<0.05) and onion fractions dried by freeze dryer showed a higher inhibitory effect compared with those dried by low temperature vacuum dryer. In order to determine a protective effect on H2O2-induced oxidative stress, DCHF-DA (dichlorodihydrofluorescin diacetate) assay was conducted. All fractions including crude extracts of dried onion appeared to significantly reduce the levels of intracellular reactive oxygen species (ROS) (p<0.05). Higher antioxidant effect was observed in onions dried by the low temperature vacuum dryer method. These results indicate that the low temperature vacuum dryer is useful to dry and produce onion powder.

키워드

참고문헌

  1. Block, E. 1986. Antithrombotic organosulfur compounds from garlic. J. Am. Soc. 108, 1045-1050.
  2. Bravo, L. 1998. Polyphenols: chemistry, dietary sources, metabolism and nutritional significance. Nutr. Reviews 51, 317-333.
  3. Cha, J. Y. and J. Y. Han. 2002. The Antioxidant ability of grape seed extracts. Kor. J. Food Sci. Technol. 34, 524-528.
  4. Chung, H. D. 1982. Control of onion bulb rot during storage at low temperature by postharvest treatment of fungicides. J. Korean Soc. Hort. Sci. 23, 17-22.
  5. Da-Mota, V. M. and E. Palau. 1999. Acoustic drying of onion. Drying Technol. 17, 855-867. https://doi.org/10.1080/07373939908917574
  6. Denizot, F. and R. Lang. 1986. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliablity. J. Immunol. Methods 89, 271-277. https://doi.org/10.1016/0022-1759(86)90368-6
  7. Freucenbeim, J. L., J. R Marshall, J. E. Vena, R. Laughlin, J. R. Brasure, M. K. Swanson, T. Nemoto and S. Graham. 1996. Premenopausal breast cancer risk and intake of vegetables, fruits and realted nutrients. J. Natl. Cancer. Inst. 88, 340-348. https://doi.org/10.1093/jnci/88.6.340
  8. Kang, N. S., J. H. Kim and J. K. Kim. 2007. Modification of quality characteristics of onion powder by hot-air, vacuum and freeze drying methods. Kor. J. Food Preserv. 18, 1-5.
  9. Kim, H. K., H. C. Lee, M. H. Park and D. H. Shin. 1986. Microflora of decayed onion bulbs and their suppression by fumigation treatment. Kor. J. Food Sci. Technol. 18, 1-5.
  10. Kim, H. R., E. J. Seog, J. H. Lee and J. W. Rhim. 2007. Physicochemical properties of onion powder as influenced by drying methods. J. Kor. Soc. Food Sci. Nutr. 36, 342-347. https://doi.org/10.3746/jkfn.2007.36.3.342
  11. Kim, K. K. 1999. Thermal characteristics of agriculture and fisheries by low temperature vacuum dryer. Proceedings of the KSME 1999 Spring Annual Meeting 1-6.
  12. Kim, K. K., B. Y. Sung, H. S. Jung, S. Y. Choi and S. B. Moon. 2000. A study on the thermal characteristics of the large low temperature vacuum dryer for biological drying. J. Kor. Soc. Marine Engineers 24, 427-434.
  13. Kim, K. M., H. J. Suh, S. H. Chung, W. D. Cho and S. J. Ma. 1999. Chemical structure of angiotensin converting enzyme inhibitor isolated from onion flesh. Food Sci. Biotechnol. 8, 329-332.
  14. Kim, S. O. and M. Y. Lee. 2001. Effect of ethyl acetate fraction of onion on lipid metabolism in high cholesterol fed rats. J. Kor. Soc. Food Sci. Nutr. 30, 673-678.
  15. Kim, Y. H, M. Y. Shon and N. J. Sung. 2004. Antioxidant and antimutagenic activities of hot water extract from white and yellow onions after simulated gastric digestion. J. Life Sci. 14, 925-930. https://doi.org/10.5352/JLS.2004.14.6.925
  16. Kwark, H. J., Y. J. Kwon, P. H. Jeong, J. H. Kwon and H. K. Kim. 2000. Physiological activity and antioxidantive effect of methanol extract from onion (Allium cepa L.). Kor. J. Food Sci. Nutr. 29, 349-355.
  17. LeBel, C. P., H. Ischiropoulos and S. C. Bondy. 1992. Evaluation of the probe 2', 7' -dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress. Chem. Res. Toxicol. 5, 227-231. https://doi.org/10.1021/tx00026a012
  18. Lee, C. J., H. D. Kim, E. H. Choung, J. K. Suh, C. W. Park and J. K. Ha. 2000. Reduction effect of carcinogen induced mouse epidermal and forestomach carcinogenesis by the extract of onion wastes. J. Kor. Soc. Food Sci. Nutr. 29, 525-530.
  19. Lee, H. C., H. K. Kim, M. H. Park and D. H. Shin. 1984. Confirmation of saprophytes of onions in Korea and effects of temperature, humidity and fumigation on Boyrtis-rot. Kor. J. Appl. Miceobiol. Bioeng. 12, 299-304.
  20. Lee, K. J., S. J. Hwang, J. H. Choi and H. G. Jeong. 2008. Saponins derived from the roots of Platycodon grandiflorum inhibit HT-1080 cell invasion and MMPs activities: Regulation of NF-${\kappa}$B activiation via ROS signal pathway. Cancer Letters 268, 233-243. https://doi.org/10.1016/j.canlet.2008.03.058
  21. Lee, S. H, S. J. Woo, Y. J. Koo and H. K. Shin. 1995. Effects of mugwort, onion and polygalate radix on the intestinal environment of rats. Kor. J. Food Sci. Technol. 27, 598-604.
  22. Ma, S. J. 2000. Inhibitory effect of onion seasoning on angiotensin converting enzyme. J. Kor. Soc. Food Sci. Nutr. 29, 395-400.
  23. Miean, K. H. and S. Mohamed. 2001. Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plant. J. Agric. Food Chem. 49, 3106-3112. https://doi.org/10.1021/jf000892m
  24. Millen, A. E., A. F. Subar, B. I. Graubard, U. Peters, R. B. Hayes, J. L. Weissfeld, L. A. Yokochi and R. G. Ziegler; PLCO Cancer Screening Trial Project Team. 2007. Fruit and vegetable intake and prevalence of colorectal ad-enoma in a cancer screening trial. Am. J. Clin. Nutr. 86, 1754-1764.
  25. Park, J. and M. K. Kim. 1991. Effects of onion flesh or peel feeding on antioxidantive capacity in 16-month-old rats high iron diet. Kor. J. Food Culture 20, 721-730.
  26. Park, P. S., B. R. Lee and M. Y. Lee. 1991. Effects of onion diet on carbon tetrachloride toxicity of rats. J. Kor. Food Nutr. 20, 121-125.
  27. Park, P. S., B. R. Lee and M. Y. Lee. 1994. Effects of onion juice on ethanol-induced hepatic lipid peroxidation in rats. J. Kor. Food Nutr. 23, 750-756.
  28. Ra, K. S., H. J. Suh, S. H. Chung and J. Y. Son. 1997. Antioxidant activity of solvent extract from onion skin. Kor. J. Food Sci. Technol. 29, 595-600.
  29. Rao, R. R., S. S. Rao and P. R. Venkataraman. 1946. Investigations on plant antibiotics, studies on allicin, the antibacterial principles of Allium sativum L (Garlic). J. Sci. Ind. Res. 18, 31-35.
  30. Rho, S. N. and J. H. Han. 2000. Cyotoxicity of garlic and onion methanol extract on human lung cancer cell lines. J. Kor. Soc. Food Sci. Nutr. 29, 879-874.
  31. Sankaranrayanan, R., C. Varghese, S. W. Duff, G. Padmakumary and N. E. Day. 1995. A case-control study of diet and lung cancer in Keraka, South India. Int. J. Cancer 75, 1766-1777.
  32. Sheo, H. J., H. J. Lim and D. L. Jung. 1993. Effect of onion juice on toxicity of lead in rat. J. Kor. Soc. Food Sci. Nutr. 22, 138-143.
  33. Tsuchiya, M., M. Suematsu and H. Suzuki. 1994. In Vivo visualization of oxygen radical-dependent photoemission, Methods Enzymol. 233, 128-140. https://doi.org/10.1016/S0076-6879(94)33015-8
  34. Woo, H. S., B. J. An, J. H. Bae, S. Kim, H. J. Choi, H. S. Han and C. Choi. 2003. Effect of biologically active fractions from onion on physiological activity and lipid metabolism. J. Kor Soc. Food Sci. Nutr. 32, 119-123. https://doi.org/10.3746/jkfn.2003.32.1.119

피인용 문헌

  1. Observation for drying non-uniformity of allium vegetables using NIR spectroscopy vol.21, pp.6, 2014, https://doi.org/10.11002/kjfp.2014.21.6.838