Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Anticancer Effect of Combination of Genistein and Photofrin PDT in Human AMC-HN3 Head and Neck Cancer Cell Lines

AMC-HN3 인체 두경부 암세포에서 genistein과 photofrin PDT의 병행처리에 의한 세포 독성능의 증가

  • Kang, Jung-Wook (Medical Laser and Device Research Center, Dankook University, Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Chung, Phil-Sang (Medical Laser and Device Research Center, Dankook University, Department of Otolaryngology-Head and Neck surgery, College of Medicine, Dankook University) ;
  • Shin, Jang-In (Medical Laser and Device Research Center, Dankook University) ;
  • Son, Seung-Yeol (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Ahn, Jin-Chul (Medical Laser and Device Research Center, Dankook University, Department of Otolaryngology-Head and Neck surgery, College of Medicine, Dankook University)
  • 강정욱 (단국대학교 의학레이저 의료기기 지역혁신센터, 단국대학교 첨단과학대학 미생물학과 및 기초과학연구소) ;
  • 정필상 (단국대학교 의학레이저 의료기기 지역혁신센터, 단국대학교 의과대학 이비인후과교실) ;
  • 신장인 (단국대학교 의학레이저 의료기기 지역혁신센터) ;
  • 손승렬 (단국대학교 첨단과학대학 미생물학과 및 기초과학연구소) ;
  • 안진철 (단국대학교 의학레이저 의료기기 지역혁신센터, 단국대학교 의과대학 이비인후과교실)
  • Published : 2008.09.30
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Abstract

Photodynamic therapy (PDT) is a treatment utilizing the generation of singlet oxygen and other reactive oxygen species (ROS), which selectively accumulated in target cells. Genistein, soy-derived phytoestrogen, is one of the anticancer agents found in soybean. In the current study, we investigated the effect of photofrin-induced PDT and genistein on apoptotic cell death in head and neck cell line (AMC-HN3) to confirm the photodynamic therapy of genistein. It was determined by MTT assay that the combination group had more cytotoxicity effect than PDT group alone. Combination of photofrin PDT and genistein induced apoptosis more when comparing with PDT alone. Our data also showed that ROS was increased in combination therapy, indicating apoptosis by mitochondrial damage. These results indicated that the combination of photofrin PDT and genistein showed more cytotoxic effect and induced apoptosis in head and neck cancer cell line.

본 연구에서는 두경부 암세포 주에서 genistein의 증식억제 효과를 알아보고, genistein을 광역학 치료와 병행 치료 시 나타나는 세포 독성능 및 세포고사 유발에 대해 연구 하였다. 두경부 암세포 주에서 genistein의 세포독성능을 확인한 결과, 농도 및 시간 의존적으로 세포 독성능이 나타났다. 이에 genistein을 photofrin을 이용한 광역학 치료와 병행 치료 시 더 많은 세포 독성능이 나타났다. Genistein과 광역학 치료의 병행 치료 시 나타나는 형태적 변화는 광역학 치료시에는 주로 세포괴사의 형태가 관찰 되었으나, genistein과 병행 치료 시에는 세포고사가 관찰 되었다. 시간별로 관찰하였을 때, 광역학 치료를 $IC_{80}$으로 처리하였을 때 3시간 때에 apoptotic body가 약 3배정도 증가하였다. 세포 내 ROS를 확인한 결과 genistein과 광역학 치료를 단독으로 처리하였을 때 세포 내 존재하는 ROS가 각각 증가 하였으며, 병행 치료하였을 때는 더 많은 ROS가 형성되는 것으로 나타났다. 즉, genistein이 세포 내 ROS를 유발하여 mitochondria가 손상되고, 또한 photofrin에 의한 광역학 치료도 mitochondria를 손상시켜 세포고사를 유도하는 것으로 추측되어 진다.

Keywords

References

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