Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Lack of Association between Tumor Necrosis Factor-α -308 and -238 Promoter Polymorphisms and Chronic Hepatitis B Virus Infection

종양괴사인자-α -308과 -238 promoter 다형성과 만성 B형 간염 바이러스 감염 간의 연관성 결여

  • Jang, Won-Hee (Department of Biochemistry, College of Medicine, Inje University, Paik Institute for Clinical Research, Inje University) ;
  • Yang, Young-Il (Paik Institute for Clinical Research, Inje University,Department of Pathology, College of Medicine, Inje University) ;
  • Lee, Youn-Jae (Department of Internal Medicine, College of Medicine, Inje University) ;
  • Chun, Jin-Ho (Department of Preventive Medicine, College of Medicine, Inje University) ;
  • Yea, Sung-Su (Department of Biochemistry, College of Medicine, Inje University) ;
  • Seog, Dae-Hyun (Department of Biochemistry, College of Medicine, Inje University) ;
  • Kim, Hyeong-In (Paik Institute for Clinical Research, Inje University)
  • 장원희 (인제대학교 의과대학 생화학교실, 인제대학교 의과대학 백인제기념임상의학연구소) ;
  • 양영일 (인제대학교 의과대학 백인제기념임상의학연구소, 인제대학교 의과대학 병리학교실) ;
  • 이연재 (인제대학교 의과대학 내과학교실) ;
  • 전진호 (인제대학교 의과대학 예방의학교실) ;
  • 예성수 (인제대학교 의과대학 생화학교실) ;
  • 석대현 (인제대학교 의과대학 생화학교실) ;
  • 김형인 (인제대학교 의과대학 백인제기념임상의학연구소)
  • Published : 2008.09.30
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Abstract

The pro-inflammatory cytokine tumor necrosis factor-$\alpha$ (TNF-$\alpha$) is an important mediator of the immune response in hepatitis B virus (HBV) infection. Since the production of TNF-$\alpha$ is mostly regulated at the transcriptional level and polymorphisms in the TNF-$\alpha$ promoter alter its expression, TNF-$\alpha$ promoter polymorphisms could affect the pathogenesis of chronic HBV infection. In this study, we investigated the potential association of TNF-$\alpha$ promoter polymorphisms with chronic HBV infection. The study included 181 patients with chronic HBV infection, 201 persons who had been spontaneously recovered from hepatitis B, and 170 unrelated healthy controls. The -308G/A and -238G/A polymorphisms in the TNF-$\alpha$ promoter were analyzed by PCR-restriction fragment length polymorphism. The distribution of both the -308 and -238 genotypes in the patient group was not statistically different from that in the spontaneous recovery and control groups (p>0.05). There was also no significant difference in the allele frequency between the groups (p>0.05). The results suggest that the TNF-$\alpha$ -308 and -238 promoter polymorphisms are not associated with the development of chronic HBV infection in the Korean population.

Pro-inflammatory cytokine인 종양괴사인자-$\alpha$ (TNF-$\alpha$)는 B형 간염바이러스(HBV) 감염에 대한 면역반응의 중요한 매개인자이다. TNF-$\alpha$의 생산은 전사수준에서 주로 조절되며, TNF-$\alpha$ promoter 다형성은 TNF-$\alpha$ 유전자 발현을 변화시키기 때문에, TNF-$\alpha$ promoter 다형성이 만성 HBV 감염의 병태생리에 영향을 미칠 가능성이 높다. 그러므로, 본 연구에서는 TNF-$\alpha$ promoter 다형성과 만성 HBV 감염의 연관성을 조사하였다. 본 연구는 만성 HBV 감염환자 181명, HBV 감염이 자연적으로 치유된 201명, 그리고 건강한 대조군 170명을 대상으로 하였다. TNF-$\alpha$ promoter의 -308G/A와 -238G/A 다형성은 PCR-RFLP법을 통하여 분석하였다. 분석결과, 환자군에서 -308과 -238 유전자형의 분포는 자연치유군 및 건강대조군에서의 분포와 통계적인 차이를 보이지 않았다(p>0.05). 또한, 그룹 간에 allele frequency도 통계적 차이가 없었다(p>0.05). 따라서, 본 연구의 결과는 한국인에서 TNF-$\alpha$ -308과 -238 promoter 다형성은 만성 B형 간염 바이러스 감염의 발생과 연관성이 없음을 시사한다.

Keywords

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