The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Determination of Rosmarinic Acid and Caffeic Acid from Perilla frutescens var. japonica and var. acuta by Reversed-Phase HPLC

RP-HPLC를 이용한 백소엽(白蘇葉)과 자소엽(紫蘇葉)의 카페익산과 로즈마린산 분석

  • Kim, Byung-Youn (Dept. of Oriental Pharmaceutical Sciences, Kyung Hee University) ;
  • Jeong, Ji-Seon (Dept. of Oriental Pharmaceutical Sciences, Kyung Hee University) ;
  • Kwon, Ha-Jeong (Dept. of Oriental Pharmaceutical Sciences, Kyung Hee University) ;
  • Lee, Je-Hyun (Koren Medicine Research Center, Dongguk University) ;
  • Hong, Seon-Pyo (Dept. of Oriental Pharmaceutical Sciences, Kyung Hee University)
  • Published : 2008.09.30
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Abstract

Objectives : This study presents a high performance liquid chromatography methods for the quantitative and qualitative analysis of rosmarinic acid (RA) and caffeic acid (CA) in Perilla frutescens var. japonica and var. acuta. Methods : Chromatographic separation was performed using a mixture of methanol, water and formic acid (35 : 64.2 : 0.8) with a reversed-phase column (Gemini C18, 4.6 ${\times}$ 150 mm, 3 ${\mu}m$). The analyses were detected at UV (280 nm). Results : The samples were extracted with 50% EtOH under reflux for 1 h, and simultaneous determination for RA and CA in hyang-so-san and haeng-so-san was possible without interference peaks Conclusions : According the results, we developed a determination method for RA and CA in Perillae Folium. Owing to Perilla frutescens var. japonica and var. acuta did not show significant difference in contents of RA and CA, both Perilla frutescens could be available as herbal medicine.

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