The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지

Protective Effects of Thujae Semen against Neurotoxicity Induced by 6-hydroxydopamine in PC12 Cells

백자인의 6-하이드록시도파민으로 유도된 뇌세포독성에 대한 보호효과

  • Kim, Hyo-Geun (Dep. of Herbology, College of Pharmacy, Kyung Hee University) ;
  • Shim, Jin-Sup (Dep. of Herbology, College of Pharmacy, Kyung Hee University) ;
  • Ju, Mi-Sun (Dep. of Herbology, College of Pharmacy, Kyung Hee University) ;
  • Cho, Seung-Hun (Dep. of Oriental Neuropsychiatry, College of Oriental Medicine, Kyung Hee University) ;
  • Oh, Myung-Sook (Dep. of Herbology, College of Pharmacy, Kyung Hee University)
  • 김효근 (경희대학교 약학대학 본초학교실) ;
  • 심진섭 (경희대학교 약학대학 본초학교실) ;
  • 주미선 (경희대학교 약학대학 본초학교실) ;
  • 조성훈 (경희대학교 한의과대학 한방신경정신과) ;
  • 오명숙 (경희대학교 약학대학 본초학교실)
  • Published : 2008.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study was performed to evaluate the neuroprotective effect of water extracts from Thujae Semen(TSW) in PC12 cells. Methods : We performed 2,2-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging assay, 2,2-azinobis- (3-ethyl-benzothiazoline-6-sulfonic acid(ABTS) cation scavenging assay, and determination of total polyphenolic content to examine the antioxidant effects of TSW. We also carried out 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay(MTT), reactve oxygen species(ROS) assay, and nitric oxide(NO) assay to examine neuroprotective effects against 6-hydroxydopamine(6-OHDA) in PC12 cells. Results : TSW showed $IC_{50}$ values of 404.3 and 219.9 ${\mu}g/mL$ in DPPH and in ABTS assays, respectively. TSW showed 9.74 ${\mu}g/mL$ of total polyphenol contents. TSW incresed cell viability in a dose dependent manner and it showed protective effect against 6-OHDA neurotoxicity at the concentration of 25-200 ${\mu}g/mL$. Moreover, it recovered 6-OHDA induced cell death at the same concentrations. The extract showed a dose dependent reduction of ROS and NO generation by 6-OHDA. Conclusions : We concluded that TSW has neuroprotective effect against 6-OHDA-induced toxicity in PC12 cells through ROS and NO inhibition.

Keywords