대한본초학회지 (The Korea Journal of Herbology)
- 제23권3호
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- Pages.1-9
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- 2008
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- 1229-1765(pISSN)
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- 2288-7199(eISSN)
광두근(廣豆根)의 Gerbil 전뇌(全腦)허혈에 대한 신경손상방어효능 연구
Neuroprotecticve Effect of Sophora Subprostrata on Transient Global Ischemia in Gerbil
- 민홍규 (경희대학교 한의과대학 한의학연구소) ;
- 강호창 (경희대학교 동서의학대학원) ;
- 이현삼 (경희대학교 한의과대학 한의학연구소) ;
- 김선여 (경희대학교 동서의학대학원) ;
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손영주
(상지대학교 한의과대학) ;
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정혁상
(경희대학교 한의과대학 한의학연구소) ;
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손낙원
(경희대학교 동서의학대학원) ;
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김윤범
(경희대학교 한의과대학 한의학연구소)
- Min, Hong-Kyu (Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
- Kang, Ho-Chang (Graduate School of East-West Medicine, Kyung Hee University) ;
- Lee, Hyun-Sam (Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
- Kim, Sun-Yeou (Graduate School of East-West Medicine, Kyung Hee University) ;
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Sohn, Young-Joo
(College of Oriental Medicine, Sangji University) ;
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Jung, Hyuk-Sang
(Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
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Sohn, Nak-Won
(Graduate School of East-West Medicine, Kyung Hee University) ;
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Kim, Yoon-Bum
(Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University)
- 발행 : 2008.09.30
초록
Objectives : This research was performed to investigate protective effect of Sophora Subprostrata against transient global ischemic damage after 5-min two vessel occlusion. Methods : Gerbils were divided into three groups: Normal group, 5-min two vessel occlusion (2VO) group, Sophora Subprostrata administrated group after 2VO. The CCAs were occluded by microclip for 5min. Sophora Subprostrata was administrated orally(12mg/ml) for 7 days after 2VO. The histological and immunohistochemistrical analysis was performed at 72 hours and 7 days after the surgery each. For histological analysis, the brain tissue was stained with 1% cresyl violet solution and Immunohistochemistry for BAX and Bcl-2 was carried out to examine effect of Sophora Subprostrata on ischemic brain tissue. Results : The results showed that (1) Sophora Subprostrata has the protective effect against ischemia in CA1 area of the gerbil hippocampus 7 days after 5-minute occlusion, (2) the treatment of Sophora Subprostrata inhibits the expression of Bax relatively after 2VO-induced ischemia. That protective effect of the Sophora Subprostrata seems to be performed by regulating the proportion of Bax and Bcl-2 protein, (3) in hypoxia/reperfusion model using PC12 cell, the Sophora Subprostrata extract has the protective effect against ischemia in the dose of