The Journal of Korean Medicine (대한한의학회지)
- Volume 29 Issue 4
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- Pages.171-179
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- 2008
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- 1010-0695(pISSN)
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- 2288-3339(eISSN)
Screening of the Bufonis Venenum on Hep G2 Cells
섬여가 간암(肝癌) 세포주 Hep G2에 미치는 효과
- Kang, A-my (Department of Internal Medicine, College of Korean Medicine, Oriental Medicine) ;
- Kim, Bo-Ram (Department of Internal Medicine, College of Korean Medicine, Oriental Medicine) ;
- Kim, Sung-Uk (Department of Internal Medicine, College of Korean Medicine, Oriental Medicine) ;
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Lim, Seong-Woo
(Department of Internal Medicine, College of Korean Medicine, Oriental Medicine)
- 강아미 (동국대학교 일산한방병원 한방내과) ;
- 김보람 (동국대학교 일산한방병원 한방내과) ;
- 김승욱 (동국대학교 일산한방병원 한방내과) ;
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임성우
(동국대학교 일산한방병원 한방내과)
- Published : 2008.09.30
Abstract
Objective: Bufonis Venenum is the traditional Korean medicine Chan Su, which is obtained from the skin and parotid venom gland of the toads. It has been used for myocardial diseases, inflammation diseases, pain relief, cancer and others. The main components of BV are cinobufotoxin, cinobufalin, bufalin and others. Of these, bufalin, the major active ingredient of BV, has been reported to induce apoptosis and to possess anti-tumor effects. There was no report of anti-tumor screening of BV on hepatic cancer and which signaling pathway can be involved. In order to examine the effect of BV on hepatic cancer and the related signaling pathway with BV-induced apoptosis, human Hep G2 cells were used. Methods: Analysis of apoptosis was confirmed by MTT assay. BV decreased cell viability in a dose and duration dependent manner. To observe which signaling molecules will be activated by BV, phosphorylation of MAPK (p38, ERK, JNK), caspase 8 and caspase 9 were examined by Western blot analysis. Results: The phosphorylation levels of p38 started to increase at 5 min after addition of 5