Advances in Traditional Medicine

  • 제8권3호
  • /
  • Pages.302-310
  • /
  • 2008
  • /
  • 2662-4052(pISSN)
  • /
  • 2662-4060(eISSN)
경희대학교 융합한의과학연구소 (Kyung Hee Oriental Medicine Research Center)
권호 표지
DOI QR코드

DOI QR Code

Effect of Azadirachta indica flower extract on basal and experimentally elevated blood glucose in rats

  • Waliullah, S (Department of Ilmul Advia, Faculty of Medicine (U), Jamia Hamdard) ;
  • Javed, Kalim (Department of Chemistry, Faculty of Science, Jamia Hamdard) ;
  • Jafri, MA (Department of Ilmul Advia, Faculty of Medicine (U), Jamia Hamdard) ;
  • Singh, S (Department of Pharmacology, All India Institute of Medical Sciences)
  • 발행 : 2008.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Azadirachta indica A. Juss (N.O. Meliaceae), popularly known, as 'Neem' is an indigenous tree widely available in India. Almost every part of the tree has long been used in Unani system of medicine for the treatment of a variety of human ailments. The flowers have been mentioned as a remedy useful in controlling diabetes mellitus. The present study had been designed to investigate the hypoglycemic/anti-hyperglycemic effects of the methanolic extract of the flowers of A. indica (Gule-Neem) and its different fractions on normal, glucose fed hyperglycemic, adrenaline induced hyperglycemic and alloxan induced diabetic rats. The methanolic extract was resolved into water soluble and water insoluble fractions. Water soluble portion of the methanolic extract was found to possess significant blood sugar lowering effect in glucose-fed and adrenaline-induced hyperglycemic rats but it did not show such effect in normal and alloxan induced mild and severe diabetic rats. Water-soluble portion was fractionated by employing the polarity criterion with ethyl acetate and butanol. The ethyl acetate fraction was further fractionated into phenolic and non-phenolic fractions. Hypoglycemic effect of these fractions was also evaluated. The results suggest that the flowers of A. indica contain at least two different constituents, responsible for the said activity. These investigations validate the use of flowers of A. indica in diabetes by Unani physicians.

키워드

참고문헌

  1. Azam K. (1899) Muheet-e-Azam vol. IV part-2, p. 177-179, Nizami press, Kanpur, India
  2. Biswas K, Chattopdhyay I, Banerjee RK, Bandyopadhyay U. (2002) Biological activities and medicinal properties of neem (Azadirachta indica). Curr. Sci. 82, 1336-1345
  3. Bopanna KN, Kannan J, Gadgie S, Balaraman R, Rathod S. (1997) Anti-diabetic and anti-hyperglycemic effect on Neem seed kernels powder on alloxan induced diabetic rabbits. Indian J. Pharm. 29, 162-167
  4. Chakraborty T, Poddar G. (1984) Herbal drugs in diabetes part: I, Hypoglycemic activity of indigenous plants in streptozotocin induced diabetic rats. J. Inst. Chem. India 56, 20-22
  5. Chakraborty T, Varotta L, Podder G. (1989) Evaluation of Azadirachta indica leaf extract for hypoglycemic activity in rats. Phytother. Res. 3, 30-32 https://doi.org/10.1002/ptr.2650030108
  6. Chattopadhyay RR. (1993) Possible mechanism of anti-hyperglycemic effect of Azadirachta indica leaf extract, part II. J. Ecobiol. 4, 309-312
  7. Chattopadhyay RR. (1996) Possible mechanism of anti-hyperglycemic effect of Azadirachta indica leaf extract, part IV. Gen. Pharmacol. 27, 431-434 https://doi.org/10.1016/0306-3623(95)02070-5
  8. Chattopadhyay RR. (1999) Possible mechanism of anti-hyperglycaemic effect of Azadirachta indica leaf extract, part V. J. Ethnopharmacol. 67, 373-376 https://doi.org/10.1016/S0378-8741(99)00094-X
  9. Chattopadhyay RR, Chattopadhyay RN, Maitra SK. (1993b) Possible mechanism of anti-hyperglycemic effect of Azadirachta indica leaf extract, part III. Fitoterapia LXIV(6), 535-538
  10. Chattopadhyay RR, Chattopadhyay RN, Nandy A, Podder G, Maitra SK. (1987) Preliminary report on anti-hyperglycemic effect of a fraction of fresh leaves of Azadirachta indica (Beng. Neem). Bull. Calcutta Sch. Trop. Med. 35, 29-35
  11. Chattopadhyay RN, Maitra SK, Chattopadhyay RR. (1993a) Possible mechanism anti-hyperglycemic effect of Azadirachta indica leaf extract, part I. Fitoterapia LXIV(4), 332-336
  12. Dixit VP, Sinha R, Tank R. (1986) Effect of Neem seed oil on the blood glucose concentration of normal and alloxan diabetic rats. J. Ethnopharmacol. 17, 95-98 https://doi.org/10.1016/0378-8741(86)90076-0
  13. Dubowski MK. (1962) Methods for body glucose determination. Clin.Chem. 8, 215-253
  14. El-Hawary ZM, Kholief TS. (1990) Biochemical studies on hypoglycemic agents (1), Effect of Azadirachta indica leaf extract. Arch. Pharmacol. Res. 13, 108-112 https://doi.org/10.1007/BF02857845
  15. Feldman JM, Libovitz HE. (1970) Mechanism of epinephrine and serotonin inhibition of insulin release in golden hamster in vitro. Diabetes 19, 480-487
  16. Ghani K. (1920) Khazain ul-Advia Vol. III, p. 988-996, Nawal Kishore, Lucknow, india
  17. Gupta S, Kataria M, Gupta PK, Murganandan S, Yashroy RC. (2004) Protective role of extracts of neem seeds in diabetes caused by streptozotocin in rats. J. Ethnopharmacol. 90, 185-189 https://doi.org/10.1016/j.jep.2003.09.024
  18. Harborne JB. (1973) Phytochemical methods, p. 1-40, Chapman and Hall, London
  19. Hussain HEMA. (2002) Reversal of diabetic retinopathy in streptozotocin induced diabetic rats using traditional Indian anti-diabetic plant, Azadirachta indica (L). Indian J. Clin. Biochem. 17, 115-123 https://doi.org/10.1007/BF02867983
  20. Kaushik A, Saxena KK, Srivastava VK, Kumar A, Singh VS. (1999) Effect of alcoholic extract of Azadirachta indica (Neem) on blood glucose and lipid profile in experimentally induced diabetes. Indian J. Pharmacol. 31, 56
  21. Luscombe DK, Taha SA. (1974) Pharmacological Studies on the leaves of Azadirachta indica. J. Pharm. Pharmacol. 26, 111
  22. Murthy KS, Rao D.N, Rao DK, Murthy LBG. (1978) A preliminary study on hypoglycemic and antihyperglycemic effect of Azadirachta indica. Indian J. Pharmacol. 10, 247-250
  23. Nanduri S, Banstola P. (1995) Neeflone a new tetranortriterpenoid from Flowers of Azadirachta indica. Indian J. Chem. 34B, 1019-1020
  24. Pillai NR, Santhakumari G. (1981) Hypoglycemic activity of Melia azadirachta Linn (Neem). Indian J. Med. Res. 74, 931
  25. Purohit AK, Joshi VB, Pingle VS, Vyas SA. (1990) Hypoglycemic activity of Neem seed oil (Azadirachta indica) on streptozotocin induced diabetic mice. J. Diabetes Assoc. India 30, 36-37
  26. Rahman SZ, Jairajpuri S. (1996) Unani Medicine edited by NS Randhawa, BS Parmar, p. 256-267, New Age International (P) Ltd., Calcutta, India
  27. Santoshkumari KS, Devi KS. (1990) Hypoglycemic effect of a few medicinal plants. Ancient Sci. Life 9, 221-223
  28. Sha H, Zhao JB, Zhang ZY, Zhou SP, Tong XL, Zhuang FY, Gregersen H. (2006) Effect of Kaiyu Qingwei Jianji on the morphometry and residual strain distribution of small intestine in experimental diabetic rats. World J. Gastroenterol. 12, 7149-7154
  29. Sharma MK, Khare AK, Feroz H. (1983) Effect of Neem oil on blood sugar levels of normal hypoglycemic and diabetic animals. Indian Med. Gaz. Nov., 380-383
  30. Siddiqui B, Siddiqui BS, Faizi S. (1986a) Studies on the Chemical constituents of Azadirachta indica-VII. Z. Naturforsch. 42B, 922-924
  31. Siddiqui B, Siddiqui BS, Faizi S, Mahmood T. (1986b). Isolation of triterpenoid from Azadirachta indica. Phytochemistry 25, 2183-2185 https://doi.org/10.1016/0031-9422(86)80087-5
  32. Subramanian SS, Rangaswami S. (1947) Chemical Examination of the Flowers of Melia azadirachta. Curr. Sci. 16, 182-183
  33. Subramanian SS, Nair AGR. (1972) Melicitrin - A new myricetin glycoside from the flowers of Melia azadirachta. Indian J. Chem. 10, 452