DOI QR코드

DOI QR Code

Antioxidant activities of Erythrina stricta Roxb.using various in vitro and ex vivo models

  • AsokKumar, K (Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences) ;
  • UmaMaheswari, M (Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences) ;
  • Sivashanmugam, AT (Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences) ;
  • SubhadraDevi, V (Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences) ;
  • Subhashini, N (Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences) ;
  • Ravi, TK (Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences)
  • 발행 : 2008.09.30

초록

Erythrina stricta, a deciduous tree widely used traditionally in indigenous system of medicine for various ailments such as rheumatism, fever, leprosy, epilepsy etc. The leaves of Erythrina stricta was extracted with ethanol (70%) and used for the evaluation of various in vitro antioxidant assays which includes H - donor activity, nitric oxide scavenging, superoxide anion scavenging, reducing ability, hydroxyl radical, hydrogen peroxide scavenging, total phenolic content, total flavonoid content, total antioxidant activity by thiocyanate and phosphomolybdenum method, metal chelating, $\beta$-carotene bleaching, total peroxy radical assays. The pro-oxidant activity was measured using bleomycin-dependent DNA damage. Ex vivo models like lipid peroxidation and erythrocyte haemolysis were also used to study the antioxidant property of the extract. The various antioxidant activities were compared with suitable standard antioxidants such as ascorbic acid, butylated hydroxyl toluene, $\alpha$-tocopherol, curcumin, quercetin and Trolox. The generation of free radicals viz. $O_2^{{\cdot}-}$, $OH^{\cdot}$, $H_2O_2$, $NO^{\cdot}$ and peroxyl radicals were effectively scavenged by the ethanolic extract of Erythrina stricta. In all the methods, the extract offered strong antioxidant activity in a concentration dependent manner. The total phenolic content, flavonoid content and total antioxidant activity in Erythrina stricta were determined as microgram (g) pyrocatechol, quercetin and $\alpha$-tocopherol equivalent/mg respectively. The extract did not exhibit any prooxidant activity when compared with ascorbic acid. The results obtained in the present study clearly indicates that Erythrina stricta scavenges free radicals and reduces lipid peroxidation, ameliorating the damage imposed by oxidative stress in different disease conditions and serve as a potential source of natural antioxidant.

키워드

참고문헌

  1. Agrawal PK. (1989) Carbon-13 NMR of flavonoids. New York: Elsevier
  2. Ames BN. (1983) Dietary carcinogens and anticarcinogens oxygen radicals and degenerative diseases. Science 221, 1256-1262 https://doi.org/10.1126/science.6351251
  3. Aruoma OI. (2003) Methodological considerations for characterizing potential antioxidant actions of bioactive components in plant foods. Mutat. Res. 523-524, 9-20 https://doi.org/10.1016/S0027-5107(02)00317-2
  4. Brand Williams W, Cuvelier ME, Berset C. (1995) Use of a free radical method to evaluate antioxidant activity. Lebensm. Wiss. Technol. 28, 25-30 https://doi.org/10.1016/S0023-6438(95)80008-5
  5. Cioffi G, D'Auria M, Braca A, Mendez J, Castillo A, Morelli I, De Simone F, De Tommasi N. (2002) Antioxidnt and free radical scavenging activity of constituents of the leaves of Tachigalia paniculata. J. Nat. Prod. 65, 1526-1529 https://doi.org/10.1021/np0200764
  6. Das D, Bandyopadhyay D, Bhattacharjee M, Banerjee RK. (1997) Hydroxyl radicals is the major causative factor in stress induced gastric ulceration. Free Radic. Biol. Med. 23, 8-18 https://doi.org/10.1016/S0891-5849(96)00547-3
  7. Dinis TCP, Madeira, VMC, Almeida LM. (1994) Action of phenolic derivatives (acetoaminophen, salicylate and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch. Biochem. Biophys. 315, 161-169 https://doi.org/10.1006/abbi.1994.1485
  8. Elzaawely AA, Xuan TD, Tawata S. (2005) Antioxidant and antibacterial activities of Rumex japonicus HOUTT. Aerial parts. Biol. Pharm. Bull. 28, 2225-2230 https://doi.org/10.1248/bpb.28.2225
  9. Freidovich I. (1999) Fundamental aspects of reactive oxygen species, or what's the matter with oxygen? Ann. NY. Acad. Sci. 893, 13 https://doi.org/10.1111/j.1749-6632.1999.tb07814.x
  10. Gilbert DL. (2000) Fifty years of radical ideas. Ann. NY. Acad. Sci. 899, 1
  11. Gordon MH. (1990) The mechanism of antioxidant action in vitro. In: Food antioxidants, edited by Hudson BJF, p.1-18. Elsevier Applied Science, London
  12. Gulcin I, Kufrevioglu OI, Oktay M, Buyukokuroglu ME. (2004) Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioica L.). J. Ethnopharmacol. 90, 205-215 https://doi.org/10.1016/j.jep.2003.09.028
  13. Gutierrez RMP, Luna HH, Garrido SH. (2006) Antioxidant activity of Tagetes erecta essential oil. J. Chilean Chem. Soc. 51, 883-886
  14. Guzman S, Gato A, Calleja JM. (2001) Antiinflammatory, analgesic and free radical scavenging activities of the marine microalgae Chlorella stigmatophora and Phaeodactylum tricornutum. Phytother. Res. 15, 224-230 https://doi.org/10.1002/ptr.715
  15. Halliwell B, Gutteridge JMC, Arouma OI. (1987) The deoxyribose method: a simple 'test tube'assay for determination of rate constants for reactions of hydroxyl radicals. Anal. Biochem. 165, 215-219 https://doi.org/10.1016/0003-2697(87)90222-3
  16. Haraguchi H, Inoue J, Tamura Y, Mizutani K. (2002) Antioxidative components of Psoralea corylifolia. Phytother. Res. 16, 539-544 https://doi.org/10.1002/ptr.972
  17. Hsu CY. (2006) Antioxidant activity of extract from Polygonum aviculare L. Biol. Res. 39, 281-288
  18. Huang SC, Kuo JC. (2000) Concentrations and antioxidative activity of anserine and carnosine in poultry meat extracts treated with demineralization and papain. Proc. Natl. Sci. Counc. Repub. China B 24, 193-201
  19. Illavarasan R, Mallika M, Venkataraman S. (2005) Anti-inflammatory and antioxidant activities of Cassia fistula Linn bark extracts. Afr. J. Trad. Comp. Alt. Med. 2, 70-85
  20. Javanmardi J, Stushnoff C, Locke E, Vivanco JM. (2003) Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chem. 83, 547-550 https://doi.org/10.1016/S0308-8146(03)00151-1
  21. Jayaprakasha GK, Jena BS, Negi PS, Sakariah KK. (2002) Evaluation of antioxidant activities and antimutagenicity of turmeric oil: a byproduct from curcumin production. Verlag der Zeitschrift fur Naturforschung (Z. Naturforsch., C, Biosci.) 57c, 828-835
  22. Kaur C, Kapoor HC. (2001) Antioxidants in fruits and vegetables- the millennium's, health. Int. J. Food Sci. Technol. 36, 703-725 https://doi.org/10.1046/j.1365-2621.2001.00513.x
  23. Khanam S, Shivprasad HN, Devi K. (2004) In vitro antioxidant screening models: a review. Indian J. Pharm. Edu. 38, 180-183
  24. Khandelwal KR. (2004) Practical pharmacognosy, $11^{th}$ ed., pp. 149-156. Nirali Prakashan, Pune
  25. Kim KS, Lee S, Lee YS, Jung SH, Park Y, Shin KH, Kim BK. (2003) Antioxidant activities of the extracts from the herbs of Artemisia apiacea. J. Ethnopharmacol. 85, 69-72 https://doi.org/10.1016/S0378-8741(02)00338-0
  26. Kirby AJ, Schmidt RJ. (1997) The antioxidant activity of Chinese herbs for eczema and of placebo herbs- I. J. Ethnopharmacol. 56, 103-108 https://doi.org/10.1016/S0378-8741(97)01510-9
  27. Koppenol WH. (1993) The centennial of the Fenton reaction. Free Radic. Biol. Med. 15, 645-651 https://doi.org/10.1016/0891-5849(93)90168-T
  28. Kumar RS, Sivakumar T, Sunderam RS, Gupta M, Mazumdar UK, Gomathi P, Rajeshwar Y, Saravanan S, Kumar MS, Murugesh K, Kumar KA. (2005) Antioxidant and antimicrobial activities of Bauhinia racemosa L. stem bark. Braz. J. Med. Biol. Res. 38, 1015-1024 https://doi.org/10.1590/S0100-879X2005000700004
  29. Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L. (1994) The nitric oxide scavenging property of Ginkgo biloba extract Egb 761. Biochem. Biophys. Res. Commun. 201, 748-755 https://doi.org/10.1006/bbrc.1994.1764
  30. Mates JM, Perez-Gomez C, Castro IN. (1998) Antioxidant enzymes and human diseases. Clin. Chem. 32, 595-603
  31. McCard JM. (2000) The evolution of free radicals and oxidative stress. Am. J. Med. 108, 652 https://doi.org/10.1016/S0002-9343(00)00412-5
  32. McCune LM, Johns T. (2002) Antioxidant activity in medicinal plants associated with the symptoms of diabetes mellitus used by the indigenous peoples of the north American boreal forest. J. Ethnopharmacol. 82, 197-205 https://doi.org/10.1016/S0378-8741(02)00180-0
  33. Mensor LL, Menezes FS, Leitao GG, Reis AS, dos Santos TC, Coube CS, Leitão SG. (2001) Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother. Res. 15, 127-130 https://doi.org/10.1002/ptr.687
  34. Moncada A, Palmer RMJ, Higgs EA. (1991) Nitric oxide: physiology, pathophysiology and pharmacology. Pharmacol. Rev. 43, 109-142
  35. Motalleb G, Hanachi P, Kwa SH, Fauziah O, Asmah R. (2005) Evaluation of phenolic content and total antioxidant activity in Berberis vulgaris fruit extract. J. Biol. Sci. 5, 648-653
  36. Nadkarni KM. (1954) The Indian materia medica with ayurvedic unani and home remedies, 3rd ed., pp. 509-510. Popular Prakashan, Bombay
  37. Ng TB, Liu F, Lu Y, Cheng CHK, Wang Z. (2003) Antioxidant activity of compounds from the medicinal herb Aster tataricus. Comp. Biochem. Physiol. C 136, 109-115
  38. Ng TB, Liu F, Wang ZT. (2000) Antioxidative activity of natural products from plants. Life Sci. 66, 709-723 https://doi.org/10.1016/S0024-3205(99)00642-6
  39. Nordberg J, Arner ESJ. (2001) Reactive oxygen species, antioxidants and mammalian thioredoxin system. Free Radic. Biol. Med. 31, 1287-1312 https://doi.org/10.1016/S0891-5849(01)00724-9
  40. Ohkawa H, Ohishi W, Yagi K. (1979) Assay of peroxides in animal tissue by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  41. Oktay M, Gulcin I, Kufrevioglu I. (2003) Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. Lebensm. Wiss. u. Technol. 36, 263-271 https://doi.org/10.1016/S0023-6438
  42. Oyaizu M. (1986) Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nut. 44, 307-315
  43. Prieto P, Pineda M, Aguilar M. (1999) Spectrophotometric quantification of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Biochem. 269, 337-341 https://doi.org/10.1006/abio.1999.4019
  44. Seis H. (1993) Strategies of antioxidant defence. Eur. J. Biochem. 215, 213-219 https://doi.org/10.1111/j.1432-1033.1993.tb18025.x
  45. Singh H, Chawla AS, Kapoor VK, Kumar N. (1981) Investigation of Erythrina Spp. IX. chemical constituents of Erythrina stricta bark. J. Nat. Prod. 44, 526-530 https://doi.org/10.1021/np50017a002
  46. Sivarajan VV, Balachandran I. (1994) Ayurvedic drugs and their plant sources, pp. 350-351. Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi
  47. Slinkard K, Singleton VL. (1977) Total phenol analysis: automation and comparison with manual methods. Am. J. Enol. Vitic. 28, 49-55
  48. Sreejayan N, Rao MNA. (1997) Nitric oxide scavenging by curcuminoids. J. Pharm. Pharmacol. 49, 105-107
  49. Steinberg D. (1991) Antioxidants and atherosclerosis-A current assessment. Circulation 84, 1420-1425
  50. Steinmetz KA, Potter JD. (1996) Vegetable, fruit and cancer epidemiology. Cancer Causes Control 2, 325-351 https://doi.org/10.1007/BF00051672
  51. Thompson M, Williams CR, Elliot GEP. (1976) Stability of flavonoid complexes of copper(II) and flavonoid antioxidant activity. Anal. Chim. Acta 85, 375-381 https://doi.org/10.1016/S0003-2670(01)84703-6
  52. Toda S, Ohnisho M, Kimura M, Nakashima Y. (1988) Action of curcuminoids on the hemolysis and lipid peroxidation of mouse erythrocytes induced by hydrogen peroxide. J. Ethnopharmacol. 23, 105-106 https://doi.org/10.1016/0378-8741(88)90119-5
  53. Vendemiale G, Grattagliano I, Altomare E. (1999) An update on the role of free radicals and antioxidant defense in human disease. Int. J. Clin. Lab. Res. 29, 49-55 https://doi.org/10.1007/s005990050063
  54. Yamauchi R, Kato K, Oida S, Kanaeda J, Ueno Y. (1992) Benzyl caffeate, an antioxidative compound isolated from propolis. Biosci. Biotechnol. Biochem. 56, 1321-1322
  55. Yokozawa T, Cho EJ, Hara Y, Kitani K. (2000) Antioxidative activity of green tea treated with radical initiator 2,2'- azobis(2-amidinopropane) dihydrochloride. J. Agric. Food Chem. 48, 5068-5073 https://doi.org/10.1021/jf000253b
  56. Zin M, Abdul-Hamed A, Ojman A. (2002) Antioxidant activity of extracts from mengkudu (Morinda citrifolia L.) root, fruit and leaf. Food Chem. 48, 5068-5073

피인용 문헌

  1. Anticholinesterase and antioxidant potentials of Nonea micrantha Bioss. & Reut along with GC-MS analysis vol.17, pp.1, 2017, https://doi.org/10.1186/s12906-017-2004-9