Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Effect of Citrus aurantium var amara on weight change in mice

  • Sarker, Satyajit Dey (School of Biomedical Sciences, University of Ulster) ;
  • Habibi, Bohlol (Department of Pharmacology, Faculty of Pharmacy, Tabriz University of Medical Sciences) ;
  • Sharifi, Tohid (Department of Pharmacology, Faculty of Pharmacy, Tabriz University of Medical Sciences) ;
  • Asnaashari, Solmaz (Pharmacognosy Laboratory, Drug Applied Research Center, Tabriz University of Medical Sciences) ;
  • Nahar, Lutfun (School of Biomedical Sciences, University of Ulster) ;
  • Delazar, AndAbbas (Faculty of Pharmacy and Drug Applied Research Center, Tabriz University of Medical Sciences)
  • Published : 2008.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Citrus aurantium var. amara L., commonly known as 'bitter orange' or 'sour orange', of the family Rutaceae, has traditionally been used in the treatment of various ailments, and it possesses different types of pharmacological properties. As a part of our on-going studies on the plantsfrom the Iranian flora, the extract of C. aurantium var. amara has been studied for its weight lossproperties using the mice model. While the Sep-Pak fraction, 20% methanol (MeOH) in water, of the hydro-methanolic extract of the peels of C. aurantium var. amara fruits, when injectedintraperitoneal (i.p.) at a dose of 10 mg/kg, significantly decreased the level of weight gain of the mice in comparison with control the group (P < 0.01), the Sep-Pak fraction 80% MeOH in water decreased the initial weight of mice by 0.44% in six weeks. The administration of the total extract(10 and 20 mg/kg, i.p.), and the Sep-Pak fractions, 40% and 60% MeOH in water (10 mg/kg, i.p.)did not show any significant change of weight of the test mice. Of the two active fractions, the80% MeOH in water fraction did not show any noticeable adverse effects on mice, and was therefore analysed by reversed-phase preparative high performance liquid chromatography resulting in the isolation and identification of four major components, two coumarins, meranzin hydrate (1) and bergamottin (2), and two flavonoids, xanthomicrol 5,4'-di-methyl ether (tangeritin, 3) and hymenoxin 5,7-di-methyl ether (nobiletin, 4).

Keywords

References

  1. Delazar A, Celik S, Gokturk RS, Unal O, Nahar L, Sarker SD. (2005) Two acylated flavonoids from Stachys bombycina and their free radical scavenging activity. Pharmazie 60, 878-880
  2. Delazar A, Byres M, Gibbons S, Kumarasamy Y, Nahar L, Modarresi M, Shoeb M, Sarker SD. (2004) Iridoid glycosides from Eromostachys glabra. J. Nat. Prod. 67, 1584-1587 https://doi.org/10.1021/np040044b
  3. Delazar A, Modarresi M, Shoeb M, Nahar L, Reid RG, Majinda RRT, Sarker SD. (2006a) Eremostachiin: A new furanolabdane diterpene glycoside from Eromostachys glabra. Nat. Prod. Res. 20, 167-172 https://doi.org/10.1080/13518470500047082
  4. Delazar A, Biglari F, Nazemiyeh H, Talebpour A, Imani Y, Nahar, L, Sarker SD. (2006b) GC-MS analysis of the essential oils, and the isolation of phenylpropanoid derivatives from the aerial parts of Pimpinella aurea. Phytochemistry 67, 2176-2181 https://doi.org/10.1016/j.phytochem.2006.06.012
  5. Dondon R, Bourgeois P, Fery-Forgues S. (2006) A new bicoumarin from the leaves and stems of Triphasia trifolia. Fitoterapia 77, 129-133 https://doi.org/10.1016/j.fitote.2005.11.006
  6. Firenzuoli F, Gori L, Galapai C. (2005) Adverse reaction to an adrenergic herbal extract (Citrus aurantium). Phytomedicine 12, 247-248 https://doi.org/10.1016/j.phymed.2004.02.005
  7. Ganzera M, Lanser C, Stuppner H. (2005) Simultaneous determination of Ephedra sinica and Citrus aurantium var. amara alkaloids by ion-pair chromatography. Talanta 66, 889-894 https://doi.org/10.1016/j.talanta.2004.12.045
  8. Girennavar B, Poulose SM, Jayaprakasha GK, Bhat NG, Patil, BS. (2006) Furanocoumarins from grapefruit juice and their effect on human CYP 3A4 and CYP 1B1 isoenzymes. Bioorg. Med. Chem. 14, 2606-2612 https://doi.org/10.1016/j.bmc.2005.11.039
  9. GRIN database. (2007) USDA, ARS, National Genetic Resources Program, Germplasm Resources Information Network - (GRIN), National Germplasm Resources Laboratory, Beltsville, Maryland, USA. Available on-line at: http://www.ars-grin.gov/cgi-bin/npgs/html/genform.pl
  10. Hosseinimehr SJ, Karami M. (2005) Citrus extract modulates genotoxicity induced by cyclophosphamide in mice bone marrow cells. J. Pharm. Pharmacol. 57, 505-509 https://doi.org/10.1211/0022357055849
  11. ISI Database. (2008) ISI Web of Knowledge Service for UK Education, Thompson Scientific, UK. Available on-line at: http://wok.mimas.ac.uk/
  12. Jahaniani F, Ebrahimi SA, Rahbar-Roshandel N, Mahmoudian M. (2005) Xanthomicrol is the main cytotoxic component of Dracocephalum kotshyii and a potential anti-cancer agent. Phytochemistry 66, 1581-1592 https://doi.org/10.1016/j.phytochem.2005.04.035
  13. Mabry TJ, Markham KR, Thomas MB. (1970) The Systematic Identification of Flavonoids, Spinger-Verlag, New York
  14. Mattoli L, Cangi F, Maidecchi A, Ghiara C, Tubaro M, Traldi P. (2005) A rapid liquid chromatography electrospray ionization mass spectrometry(n) method for evaluation of synephrine in Citrus aurantium L. samples. J. Agric. Food Chem. 53, 9860-9866
  15. Muller AH, Degaspari LRO, Vieira PC, Da Silva MFDGF, Fernandes JB, Pitani JR. (1993) 3-Methoxyfuranocoumarins from Pilocarpus riedelianus. Phytochemistry 34, 585-586 https://doi.org/10.1016/0031-9422(93)80055-W
  16. Nagase H, Omae N, Omori A, Nakagawasai O, Tadano T, Yokosuka A, Sashida Y, Mimaki Y, Yamakuni T, Ohizumi Y. (2005) Nobiletin and its related flavonoids with CRE-dependent transcriptionstimulating and neuritegenic activities. Biochem. Biophys. Res. Comm. 337, 1330-1336 https://doi.org/10.1016/j.bbrc.2005.10.001
  17. Phytochemical and Ethnobotanical databases. (2007) USDA, ARS, Beltsville Agricultural Research Center, Beltsville, Maryland, USA. Available on-line at: http://www.ars-grin.gov/cgi-bin/duke/ethnobot.pl
  18. Piattelli M, Impellizzeri G. (1971) Fungistatic flavones in the leaves of citrus species resistant and susceptible to deuterophoma tracheiphila. Phytochemistry 10, 2657-2659 https://doi.org/10.1016/S0031-9422(00)97261-3
  19. Waddell TG. (1973) Hymenoxin: A flavone from Helianthus angustifolius. Phytochemistry 12, 2061

Cited by

  1. Vahl vol.25, pp.1, 2011, https://doi.org/10.1080/14786411003754330
  2. Antibacterial and free-radical-scavenging properties of Stachys schtschegleevii (Lamiaceae) vol.62, pp.4, 2010, https://doi.org/10.2298/ABS1004941A