Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Mutanase Induction in Trichoderma harzianum by Cell Wall of Laetiporus sulphureus and its Application for Mutan Removal from Oral Biofilms

  • Wiater, Adrian (Department of Industrial Microbiology, Maria Curie-Sklodowska University) ;
  • Szczodrak, Janusz (Department of Industrial Microbiology, Maria Curie-Sklodowska University) ;
  • Pleszczynska, Malgorzata (Department of Industrial Microbiology, Maria Curie-Sklodowska University)
  • Published : 2008.07.31
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Abstract

The cell wall material from fruiting bodies of Laetporus sulphureus has been suggested as a new alternative to mutan for the mutanase induction in Trichoderma harzianum. Structural analyses revealed that the cell wall fraction from this polypore fungus contained 56.3% of (1$\rightarrow$3)-linked $\alpha$-glucans. When the strain T. harzianum F-340 was grown on a cell wall preparation from L. sulphureus, the maximal enzyme productivity obtained after 3 days of cultivation was 0.71 U/ml. This yield was about 1.8-fold higher than that achieved on mutan, known so far as the best, but expensive and inaccessible, inducer of mutanase production. Cell-wall-induced mutanase showed a high hydrolytic potential in reaction with a dextranase-pretreated mutan, where maximal degrees of saccharification and solubilization of this biopolymer (80% and 100%, respectively) were reached in 3 h at 45$^{\circ}C$. The mutanase preparation was also effective in degradation of streptococcal mutan and its removal from oral biofilms, especially in a mixture with dextranase.

Keywords

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