Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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CBT-SL5, a Bacteriocin from Enterococcus faecalis, Suppresses the Expression of Interleukin-8 Induced by Propionibacterium acnes in Cultured Human Keratinocytes

  • Lee, Ye-Jin (Department of Dermatology, College of Medicine, The Catholic University of Korea) ;
  • Choi, Hye-Jeong (Department of Dermatology, College of Medicine, The Catholic University of Korea) ;
  • Kang, Tae-Wook (Department of Dermatology, College of Medicine, The Catholic University of Korea) ;
  • Kim, Hyung-Ok (Department of Dermatology, College of Medicine, The Catholic University of Korea) ;
  • Chun, Myung-Jun (Research Institute of Cell Engineering, Cell Biotech Co., Ltd.) ;
  • Park, Young-Min (Department of Dermatology, College of Medicine, The Catholic University of Korea)
  • Published : 2008.07.31
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Abstract

Propionibacterium acnes is known to playa pivotal role in the pathogenesis of acne vulgaris. CBT-SL5 is one of the antimicrobial peptides from Enterococcus faecalis SL5, and it has shown antimicrobial activity against P. acnes. The aim of this study was to investigate the anti-inflammatory effect of CBT-SL5 on the inflammation induced by P. acnes in cultured human keratinocyes. Cultured human keratinocytes derived from neonatal foreskin were treated with heat-killed P. acnes to induce inflammation, and then various concentrations of CBT-SL5 were added to the P. acnes-treated keratinocytes. The mRNA expression and protein secretion of interleukin (IL)-8, an inflammation marker, was analyzed by real-time reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. We also analyzed the nuclear factor-kappa B (NF-$\kappaB$) p65 translocation by performing immunofluorescent staining. P. acnes treatment up regulated the IL-8 mRNA expression in the keratinocytes, and this was brought about through both toll-like receptor (TLR)2 and TLR4. At the concentrations of 10, 50, and 100 ng/ml, CBT-SL5 significantly down regulated the P. acnes-induced IL-8 mRNA expression and protein production (p<0.05). At 6 hand 12 h of the treatment, CBT-SL5 significantly suppressed the P. acnes-induced IL-8 mRNA expression. Secretion of IL-8 protein was significantly reduced at 24 h. The functional inhibitory activity of CBT-SL5 was shown by CBT-SL5 suppressing the P. acnes-induced NF-$\kappaB$ translocation from the cytoplasm to the nucleus. These results demonstrated that CBT-SL5 suppressed the P. acnes-induced IL-8 expression in keratinocytes. Therefore, CBT-SL5 may be a novel anti-inflammatory treatment for acne.

Keywords

References

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