Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
권호 표지

The Effect of Cryoprotectants on the Survival and In Vitro-Growth of Cryopreserved Mouse Preantral Follicles

  • Kim, Dong-Hoon (Animal Biotechnology Division, National Institute of Animal Science) ;
  • Chung, Duk-Soo (Mirae Laboratory) ;
  • Lim, Hyun-Joo (Animal Biotechnology Division, National Institute of Animal Science) ;
  • Im, Gi-Sun (Animal Biotechnology Division, National Institute of Animal Science) ;
  • Lee, Hwi-Cheul (Animal Biotechnology Division, National Institute of Animal Science) ;
  • Seong, Hwan-Hoo (Animal Biotechnology Division, National Institute of Animal Science)
  • Published : 2008.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to examine more effective cryoprotectant for the cryopreservation of mouse preantral follicles. Enzymetically isolated preantral follicles from 12-day-old mice were cryopreserved by a slow freezing protocol with 1.5 M propanediol (PROH), dimethyl sulphoxide (DMSO) or glycerol (GLY) and then grown and matured in vitro for 11 days after thawing. The survival of preantral follicles immediately after freezing and thawing was not different among the PROH (68.2%), DMSO (72.4%) and GLY (72.1%). After grown and matured in vitro, the rates of survival and metaphase II oocytes were 54.9% and 36.6% for PROH which was significantly higher rates (p<0.05) compared with the rates obtained from DMSO (16.9% and 9.0%) and GLY (16.3% and 7.5%). The diameter of metaphase II oocytes from pre antral follicles frozen in PROH ($67.4{\pm}1.8\;{\mu}m$) was significantly (p<0.05) smaller than that of the fresh preantral follicles ($69.1{\pm}2.3\;{\mu}m$). The results from the present study revealed that PROH is more suitable cryoprotectant for the cryopreservation of mouse preantral follicles.

Keywords

References

  1. Anderson E, Albertini DF (1976): Gap junctions between the oocyte and companion follicle cells in the mammalian ovary. J Cell Biol 71:680-686 https://doi.org/10.1083/jcb.71.2.680
  2. Brower PT, Schultz RM (1982): Intercellular communication between granulosa cells and mouse oocytes: Existence and possible nutritional role during oocyte growth. Dev Biol 90:144-153 https://doi.org/10.1016/0012-1606(82)90219-6
  3. Candy CJ, Wood MJ, Whittingham DG (1997): Effect of cryopreservation on the survival of follicles in frozen mouse ovaries. J Repord Fert 110: 11-19 https://doi.org/10.1530/jrf.0.1100011
  4. Carroll J, Gosden RG (1993): Transplantation or frozen-thawed primordial follicles. Hum Reprod 8: 1163-1167 https://doi.org/10.1093/oxfordjournals.humrep.a138221
  5. Carroll J, Whittingham G, Wood MJ, Telfer E, Gosden RG (1990): Extra-ovarian production of mature viable mouse oocytes from frozen primary follicles. J Repord Fert 90:321-327 https://doi.org/10.1530/jrf.0.0900321
  6. Cortvrindt R, Smitz J, Van Steirteghem AC (1996): A morphological and functional study of the effect of slow freezing followed by complete in-vitro maturation of primary mouse ovarian follicles. Hum Reprod 11:2648-2655 https://doi.org/10.1093/oxfordjournals.humrep.a019187
  7. Cox SL, Shaw J, Jenkin G (1996): Transplantation of cryopreserved ovarian tissue to adult recipients in mice. J Repord Fert 107:315-322 https://doi.org/10.1530/jrf.0.1070315
  8. Eppig JJ, Schroeder AC (1989): Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation, and fertilization in vitro. Biol Reprod 41:268-276 https://doi.org/10.1095/biolreprod41.2.268
  9. Gosden RG, Baird DT, Wade JC, Webb R (1994): Restoration of fertility to oophorectomized sheep by ovarian autografts stored at -196$^{\circ}C$. Hum Reprod 9:597-603 https://doi.org/10.1093/oxfordjournals.humrep.a138556
  10. Gunasena KT, Villines PM, Crister ES, Crister JK (1997): Live births after autologous transplant of cryopreserved mouse ovaries. Hum Reprod 12:101-106 https://doi.org/10.1093/humrep/12.1.101
  11. Hartshoren GM (1997): In vitro culture of ovarian follicles. Rev Reprod 2:94-104 https://doi.org/10.1530/ror.0.0020094
  12. Heller DT, Cahill DM, Schultz RM (1981): Biochemical studies of mammalian oogenesis: Metabolic cooperativity between granulosa cells and growing mouse oocytes. Dev Biol 84:455-464 https://doi.org/10.1016/0012-1606(81)90415-2
  13. Hirao Y, Nagai T, Kubo M, Miyano T, Miyake M, Kato S (1994): In vitro growth and maturation of pig oocytes. J Repord Fert 100:333-339 https://doi.org/10.1530/jrf.0.1000333
  14. Jewgenow K, Penfold LM, Meyer HHD, Wildt DE (1998): Viability of small preantral follicles from domestic cats after cryoprotectant exposure and cryopreservation. J Repord Fert 112:39-47 https://doi.org/10.1530/jrf.0.1120039
  15. Kim DH, Ko DS, Lee HC, Lee HJ, Park WI, Kim SS, Park JK, Yang BC, Park SB, Chang WK, Lee HT (2004): Comparison of maturation, fertilization, development and gene expression of mouse oocytes grown in vitro and in vivo. J Assist Reprod Genet 21:233-240 https://doi.org/10.1023/B:JARG.0000042008.83699.cc
  16. Kim DH, Seong HH, Lee HJ (2008): Factors influencing the culture of the mouse preantral follicles in vitro. Asian-Aust J Anim Sci 21:532-537 https://doi.org/10.5713/ajas.2008.70503
  17. Lin TC, Yen JM, Kuo TC, Gong KB, Hsu KH, Hsu TT (2008): Comparison of the developmental potential of 2-week-old preantral follicles derived from vitrified ovarian tissue slices, vitrified whole ovaries and vitrified/transplanted newborn mouse ovaries using the metal surface method. BMC Biotechnol 8: 38 https://doi.org/10.1186/1472-6750-8-38
  18. Liu J, Vander Elst J, Vanden Broecke, Dhont M (2001): Live offspring by in vitro fertilization of oocytes from cryopreserved primordial mouse follicles after sequential in vivo transplantation and in vitro maturation. Biol Reprod 64:171-178 https://doi.org/10.1095/biolreprod64.1.171
  19. Parrott DMV (1960): The fertility of mice with orthotopic ovarian grafts derived from frozen tissues. J Repord Fert 1:230-241 https://doi.org/10.1530/jrf.0.0010230
  20. Paynter SJ, Fuller BJ, McGrath JJ, Bernard AG, Shaw RW (1995): Cryoprotectant permeability of mammalian oocytes. Cryobiology 32:575-576
  21. Paynter SJ, Fuller BJ, Shaw RW (1996): Temperature dependence of K-K coefficients for mouse oocytes in the presence of ethylene glycol. Cryobiology 33: 663