Effects of honeybee (Apis Mellifera L) venom and probiotic in piglets

자돈에 투여한 봉독 및 생균제의 효과

  • Han, Sang-Mi (National Institute of Agricultural Science and Technology) ;
  • Lee, Kwang-Gill (National Institute of Agricultural Science and Technology) ;
  • Yeo, Joo-Hong (National Institute of Agricultural Science and Technology) ;
  • Kweon, Hae-Yong (National Institute of Agricultural Science and Technology) ;
  • Woo, Soon-Ok (National Institute of Agricultural Science and Technology) ;
  • Oh, Baeg-Young (Icheon-si Agricultural Technology Service Center) ;
  • Baek, Ha-Ju (Gyeongbuk Government Public Institute of Health and Environmental) ;
  • Chang, Young-Chae (Department of Pathology, Catholic University of Daegu School of Medicine) ;
  • Park, Kwan-Kyu (Department of Pathology, Catholic University of Daegu School of Medicine) ;
  • Kim, Soon-Tae (Northern-branch Gyeongbuk Veterinary Service Laboratory)
  • 한상미 (농촌진흥청 농업과학기술원) ;
  • 이광길 (농촌진흥청 농업과학기술원) ;
  • 여주홍 (농촌진흥청 농업과학기술원) ;
  • 권해용 (농촌진흥청 농업과학기술원) ;
  • 우순옥 (농촌진흥청 농업과학기술원) ;
  • 오백영 (이천시 농업기술센터) ;
  • 백하주 (경상북도 보건환경연구원) ;
  • 박관규 (대구가톨릭대학교 의과대학) ;
  • 장영채 (대구가톨릭대학교 의과대학) ;
  • 김순태 (경상북도 가축위생시험소)
  • Published : 2008.06.30

Abstract

This study was conducted to evaluate the effect of honeybee venom, purified using bee venom collector, and feeding of probiotics on the body weight gain, growth rate and hematological characteristics of pigs. A total of 120 pigs were examined and divided into 4 groups 1) Control (basal diet), 2) BV (basal diet + bee venom), 3) PB (basal diet + probiotics), 4) BVPB (basal diet + BV + PB). Average daily weight gain improved significantly in all test groups, especially BVPB (P < 0.05) compared to the controls. There was a significant difference in the feed conversion rate (P < 0.05) and efficiency (P < 0.05) between BVPB and control pigs. Weight gain and survivability was higher in the tests than the controls, but white blood cell count was not. Serum total protein, albumin and IgG concentration of BVPB were slightly higher than those of controls. These results suggest that treated honeybee venom and probiotics should be used together to effectively increase the productivity of pigs.

Keywords

References

  1. Fuller R. 1989. Probiotics in man and animals. J Appl Bacteriol 66(5): 365-378 https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  2. Sairanen U, Piirainen L, Grasten S, et al. 2007. The effect of probiotic fermented milk and inulin on the functions and microecology of the intestine. J Dairy Res 74(3): 367-373 https://doi.org/10.1017/S0022029907002713
  3. Amezcua MD, Friendship R, Dewey C, et al. 2007. The effect of feeding fermented liquid whey plus dextrose inoculated with specific lactic acid bacteria of pig origin to weanling pigs challenged with Escherichia coli O149:K91:F4. Vet Ther 8(3): 209-222
  4. Niewold TA, van Dijk AJ, Geenen PL, et al. 2007. Dietary specific anti- bodies in spray-dried immune plasma prevent enterotoxigenic Escherichia coli F4 (ETEC) post weaning diarrhea in piglets. Vet Microbiol 124(3): 362- 369 https://doi.org/10.1016/j.vetmic.2007.04.034
  5. 장영효, 김종근, 김홍중, 등. 2000. 자돈에 투여한 Lacrobacillus reuteri BSA-131의 생균제 효과. 한국산업미생물학회지 28 (1): 8-13
  6. 길동용, 임종선, 전경철, 등. 2004. 지속적인 생균제의 첨가가 돼지의 성장, 영양소 이용율, 혈중요소태 질소 및 면역능력에 미치는 영향. 동물자원지 46(1): 39-48
  7. 전병수, 곽정훈, 우용희, 등. 1996. 효소, 생균 및 유카제의 첨가가 돼지의 성장과 변 아취 발생 성장에 미치는 영향. 한국영양사료학회지 38(1): 52-58
  8. Gilliland SE, Walker DK. 1990. Factors to consider when selecting a culture of Lactobacillus acidophilus as a dietary adjunct to produce a hypo- cholesterolemic effect in humans. J Dairy Sci 73(4): 905-911 https://doi.org/10.3168/jds.S0022-0302(90)78747-4
  9. Takahashi S, Egawa Y, Simojo N, et al. 2007. Oral administration of Lactobacillus plantarum strain Lq80 to weaning piglets stimulates the growth of indigenous lactobacilli to modify the lactobacillal population. J Gen Appl Microbiol 53(6): 325-332 https://doi.org/10.2323/jgam.53.325
  10. Leser TD, Knarreborg A, Worm J. 2008. Germination and outgrowth of Bacillus subtilis and Bacillus liche- niformis spores in the gastrointestinal tract of pigs. J Appl Microbiol 104(4):1025-1033 https://doi.org/10.1111/j.1365-2672.2007.03633.x
  11. Massi M, Ioan P, Budriesi R, et al. 2006. Effects of probiotic bacteria on gastrointestinal motility in guinea- pig isolated tissue. World J Gastro- enterol 12(37): 5987-5994 https://doi.org/10.3748/wjg.v12.i37.5987
  12. Matijasic BB, Stojkovic S, Rogelj I. 2006. Survival and in vivo adhesion of human isolates Lactobacillus gasseri LF221 and K7 in weaned piglets and their effects on coliforms, clostridia and lactobacilli viable counts in faeces and mucosa. J Dairy Res 73(4): 417-422 https://doi.org/10.1017/S0022029906001890
  13. 한상미, 이광길, 여주홍, 등. 2006. 봉독이 자돈의 성장 및 혈액성상 변화에 미치는 영향. 한국가축위생학회지 29(3): 287-295
  14. 김문호. 1992. 봉독요법과 봉침요법. 한국교육기획. 서울:67-141
  15. Abbadie C, Besson JM. 1994. Chronic treatments with aspirin or acetaminophen reduce both the development of polyarthritis and Fos- like immunoreactivity in rat lumbar spinal cord. Pain 57(1):45-54 https://doi.org/10.1016/0304-3959(94)90106-6
  16. Habermann E, Reiz KG. 1965. On the biochemistry of bee venom peptides, melittin and apamin. Biochem Z 343(2):192-203
  17. Vick JA, Shipman WH. 1972. Effects of whole bee venom and its fractions (apamin and melittin) on plasma cortisol levels in the dog. Toxicon 10(4):377-380 https://doi.org/10.1016/0041-0101(72)90061-X
  18. Fennell JF, Shipman WH, Cole LJ. 1967. Antibacterial action of a bee venom fraction (melittin) against a penicillin-resistant staphylococcus and other microorganisms. USNRDL -TR -67-101. Res Dev Tech Rep 5:1-13
  19. Curcio-Vonlanthen V, Schneider CH, Frutig K, et al. 1997. Molecular parameters in melittin immuno-genicity. J Pept Sci 3(4):267-276 https://doi.org/10.1002/(SICI)1099-1387(199707)3:4<267::AID-PSC106>3.0.CO;2-7
  20. Rudenko SV, Nipot EE. 1996. Modulation of melittin-induced hemo- lysis of erythrocytes. Biokhimiia 61(12):2116-2124
  21. 조성구. 2000. 생봉독 주입에 의한 가축의 다발성질환 치료법 개발. 농림부 최종보고서. 서울
  22. 조성구, 최석화, 최향순 등. 1999. 생봉독을 이용한 돼지 관절염의 치료 효과. 한국임상수의학회지 16(1): 145-149
  23. Chen J, Luo C, Li HL. 1998. The contri-bution of spinal neuronal changes to development of prolonged, tonic nociceptive responses of the cat induced by subcutaneous bee venom injection. Eur J Pain 2(4):359-376 https://doi.org/10.1016/S1090-3801(98)90034-9
  24. Piek T. 1986. Venoms of the Hymenoptera. Academic Press. London: 17-44
  25. Dunn JD, Killion JJ. 1988. Effect of melittin on pituitary adrenal respon-siveness to stress. Acta Endocrinol 119(3):339-344
  26. 민병준, 권오석, 홍종욱 등. 2005. 생약제(Miracle) 첨가가 포유모돈의 생산성, 자돈의 성장 및 혈액성상 변화에 미치는 영향. 동물자원지 47(3):371-378 https://doi.org/10.5187/JAST.2005.47.3.371
  27. Takahashi S, Egawa Y, Simojo N, et al. 2007. Oral administration of Lactobacillus plantarum strain Lq80 to weaning piglets stimulates the growth of indigenous lactobacilli to modify the lactobacillal population. J Gen Appl Microbiol. 53(6):325-332 https://doi.org/10.2323/jgam.53.325
  28. Breves G, Szentkuti L, Schroder B. 2001. Effects of oligosaccharides on functional parameters of the intes- tinal tract of growing pigs. Dtsch Tierarztl Wochenschr 108(6) :246-248
  29. West CE, Gothefors L, Granstrom M, et al. 2008. Effects of feeding probiotics during weaning on infections and antibody responses to diphtheria, tetanus and Hib vaccines. Pediatr Allergy Immunol 19(1):53-60 https://doi.org/10.1111/j.1399-3038.2007.00583.x