Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibitory Effects of Lactobacillus plantarum Lipoteichoic Acid (LTA) on Staphylococcus aureus LTA-Induced Tumor Necrosis Factor-Alpha Production

  • Kim, Han-Geun (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Lee, Seung-Yeon (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Kim, Na-Ra (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Ko, Mi-Yeon (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Lee, Jung-Min (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Yi, Tae-Hoo (Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University) ;
  • Chung, Sung-Kyun (Department of Dental Hygiene, Shinheung College) ;
  • Chung, Dae-Kyun (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University)
  • Published : 2008.06.30
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Abstract

Staphylococcus aureus is a common etiologic agent for Gram-positive sepsis, and its lipoteichoic acid (LTA) may be important in causing Gram-positive bacterial septic shock. Here, we demonstrate that highly purified LTA (pLTA) isolated from Lactobacillus plantarum inhibited S. aureus LTA (aLTA)-induced TNF-${\alpha}$ production in THP-1 cells. Whereas pLTA scarcely induced TNF-${\alpha}$ production, aLTA induced excessive TNF-${\alpha}$ production. Interestingly, aLTA-induced TNF-${\alpha}$ production was inhibited by pLTA pretreatment. Compared with pLTA, aLTA induced a strong signal transduction through the MyD88, NF-${\kappa}B$, and MAP kinases. This signaling, however, was reduced by a pLTA pretreatment, and resulted in the inhibition of aLTA-induced TNF-${\alpha}$ production. Whereas dealanylated LTAs, as well as native LTAs, contributed to TNF-${\alpha}$ induction or TNF-${\alpha}$ reduction, deacylated LTAs did not, indicating that the acyl chain of LTA played an important role in the LTA-mediated immune regulation. These results suggest that pLTA may act as an antagonist for aLTA, and that an antagonistic pLTA may be a useful agent for suppressing the septic shock caused by Gram-positive bacteria.

Keywords

References

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