Journal of Haehwa Medicine (혜화의학회지)

Research Institute of Korean Medicine (대전대학교 한의학연구소)
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The Effect of Esubwhaltongtang (ESWTT) on the changes of lymphocyte composition in spleen of collagen-induced arthritis mice model

이습활통탕(利濕活通湯)이 관절염(關節炎) 병태(病態) 동물(動物)모델에서 비장내(脾臟內) 면역세포(免疫細胞) 변화(變化)에 미치는 영향(影響)

  • Chung, Woo-Hyun (Department of Oriental Pathology, College of Oriental Medicine, Daejeon University) ;
  • Choi, Hak-Joo (Department of Oriental Pathology, College of Oriental Medicine, Daejeon University) ;
  • Yun, Mi-Young (Department of Oriental Pathology, College of Oriental Medicine, Daejeon University) ;
  • Gim, Seon-Bin (Department of Oriental Pathology, College of Oriental Medicine, Daejeon University) ;
  • Kim, Dong-Hee (Department of Oriental Pathology, College of Oriental Medicine, Daejeon University)
  • 정우현 (대전대학교 한의과대학 병리학교실) ;
  • 최학주 (대전대학교 한의과대학 병리학교실) ;
  • 윤미영 (대전대학교 한의과대학 병리학교실) ;
  • 김선빈 (대전대학교 한의과대학 병리학교실) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Published : 2008.12.31
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Abstract

To investigate the effects of ESWTT on rheumatoid arthritis in collagen-induced arthritis mice model, the animals were orally administrated with the extract following by analyzing the changes of lymphocyte composition in spleen with flow cytometry. These results suggest as follows; 1. ESWTT decreased the total cell number of spleen at 400 and 200 mg/kg about 41% and 32%, respectively. 2. ESWTT reduced the number of CD19+ cells in spleen at 400 and 200 mg/kg about 51% and 46%, respectively. 3. ESWTT down-regulated the number spleen CD3+ cells approximately 26% at 400 mg/kg-treated group. 4. ESWTT decreased the number of CD3+/CD69+ cells in spleen at 400 and 200 mg/kg about 79% and 76%, respectively. 5. ESWTT reduced the number of CD4+ and CD8+ cells in spleen, however, the results were not significant. 6. ESWTT decreased the number of CD4+/CD25+ cells in spleen at 400 and 200 mg/kg about 60% and 56%, respectively. Taken together these results, ESWTT has an efficacy on rheumatoid arthritis through inhibiting the proliferation of lymphocytes such as B and T cells.

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