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The effect of MTAD as a final root canal irrigants on the coronal bacterial leakage of obturated root canals

최종 근관세척제로서의 MTAD 근관세척제가 치관부 세균미세누출에 미치는 영향

  • Kim, Tae-Woo (Department of Conservative Dentistry, The Institute of Oral Health Science, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Chang, Seok-Woo (Department of Conservative Dentistry, The Institute of Oral Health Science, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Park, Dong-Sung (Department of Conservative Dentistry, The Institute of Oral Health Science, Samsung Medical Center, Sungkyunkwan University School of Medicine)
  • 김태우 (성균관대학교 의과대학 삼성서울병원 치과진료부 보존과) ;
  • 장석우 (성균관대학교 의과대학 삼성서울병원 치과진료부 보존과) ;
  • 박동성 (성균관대학교 의과대학 삼성서울병원 치과진료부 보존과)
  • Published : 2008.07.31

Abstract

The purpose of this study was to evaluate the effects of MTAD, EDTA and sodium hypochlorite(NaOCl) as final irrigants on coronal leakage resistance to Enterococcus faecalis. Forty extracted human maxillary molars were used in this experiment. The teeth were randomly divided into positive control group (Group 1; n = 5), negative control group (Group 2; n = 5) and three experimental groups (n = 30). In Group 3 (n = 10), the root canals were irrigated with sodium hypochlorite. In Group 4 (n = 10) and 5 (n = 10), the root canals were irrigated with sodium hypochlorite and rinsed with EDTA and MTAD, respectively. The teeth in each group were cleaned and shaped to #40 profile with .04 taper, and obturated with gutta-percha and AH-26 root canal sealer. The coronal portion of each tooth was placed in contact with inoculum of Enterococcus faecalis in Brain Heart Infusion (BHI) culture media. Each root tip was placed in a vial containing sterile culture media. The vials were placed in anaerobic chamber and observed everyday for turbidity for 180 days. Statistical analysis was performed using Fisher's Exact Test. After 180 days, Group 3, 4, and 5 showed 7, 4 and 5 leaking samples respectively. The differences in leakage resistance were not statistically significant among Group 3, 4 and 5.

본 연구의 목적은 최종 근관세척제로서의 MTAD, EDTA 그리고 차아염소산 나트륨 용액이 충전된 근관의 세균 (Enterococcus faecalis) 미세누출에 미치는 영향을 평가하기 위한 것이다. 40개의 발치된 사람의 대구치를 양성대조군 (1군 : n = 5), 음성대조군 (2군 : n = 5), 그리고 실험군 (3, 4, 5군 : n = 10)으로 각각 무작위 배정하였다. 3군에서는 근관형성시 차아염소산 나트륨만 사용하여 근관세척을 시행하였으며, 4군 및 5군에서는 차아염소산 나트륨과 함께 EDTA와 MTAD를 각각 최종 근관세척제로 사용하였다. 실험군 및 대조군의 치아들은 .06 taper를 가지는 40번 profile을 master apical file로 근관형성을 시행하였으며 gutta-percha와 AH-26 근관봉함제로 근관충전하였다. 각 치아의 치관부는 Enterococcus faecalis를 함유한 BHI 배지와 접촉되도록 하였고 치근 끝부분은 멸균된 배양액에 위치되도록 하였다. 각각의 치아는 anaerobic chamber내에 위치되었으며 180일 동안 혼탁도를 관찰하였다. 통계처리는 95% 신뢰수준에서 Fiher's exact test를 사용하였다. 180일의 관찰기간이 경과된 후, 3, 4, 5 군은 각각 7, 4, 5 개의 치아에서 미세누출이 일어났으며 이 차이는 통계적으로 유의성이 없었다.

Keywords

References

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