한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제41권3호
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  • Pages.176-181
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  • 2008
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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원양산 오징어(Illex argentinus) 내장으로부터 Endoprotease의 분획

Fractionation of Endoprotease from Viscera of the Argentina Shortfin Squid Illex argentinus

  • 김혜숙 (경상대학교 해양생명과학부/해양산업연구소) ;
  • 김진수 (경상대학교 해양생명과학부/해양산업연구소) ;
  • 허민수 (경상대학교 해양생명과학부/해양산업연구소)
  • Kim, Hye-Suk (Division of Marine Life Science/Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Jin-Soo (Division of Marine Life Science/Institute of Marine Industry, Gyeongsang National University) ;
  • Heu, Min-Soo (Division of Marine Life Science/Institute of Marine Industry, Gyeongsang National University)
  • 발행 : 2008.06.30
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초록

To evaluate the effective use of endoprotease from squid viscera as a food processing aid, various methods of fractionating endoprotease from viscera of the Argentina shortfin squid (Illex argentinus) were evaluated. The endoprotease-positive fractions of each fractionation were fraction II (30-40%, w/w) with cold acetone, fraction IV (50-60% saturation) with ammonium sulfate, fraction UF with anion exchange chromatography, and fraction II (15-24 kDa) with gel filtration. The specific activities (approximately 25 U/mg) of the fractions using ammonium sulfate and gel filtration were higher than the others. Total azocaseinolytic activity and recovery of the positive fraction using gel filtration were 806.95U and 37.82%, respectively, and were the highest among the positive fractions. Based on the results, gel filtration was the most efficient method for fractionating endoprotease from the viscera of Illex argentinus.

키워드

참고문헌

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피인용 문헌

  1. Endoprotease and Exopeptidase Activities in the Hepatopancreas of the Cuttlefish Sepia officinalis, the Squid Todarodes pacificus, and the Octopus Octopus vulgaris Cuvier vol.15, pp.3, 2012, https://doi.org/10.5657/FAS.2012.0197
  2. Debittering of Enzymatic Hydrolysate Using Exopeptidase Active Fractions from the Argentina Shortfin Squid Illex argentinus Hepatopancreas vol.47, pp.2, 2014, https://doi.org/10.5657/KFAS.2014.0135
  3. Fractionation and Enzymatic Characterization of Endoprotease and Exopeptidase from Crude Extracts of Cuttlefish Sepia officinalis Hepatopancreas vol.15, pp.4, 2012, https://doi.org/10.5657/FAS.2012.0283
  4. Optimization of Coho Salmon Hydrolysate Using Japanese Squid Liver and Its Properties vol.42, pp.11, 2013, https://doi.org/10.3746/jkfn.2013.42.11.1759
  5. Fractionation and Characterization of Protease Inhibitors from Fish Eggs Based on Protein Solubility vol.46, pp.2, 2013, https://doi.org/10.5657/KFAS.2013.0119
  6. Comparison of the Exopeptidase Activity of Fractions from Crude Extracts of Octopus Octopus vulgaris Cuvier Hepatopancreas Using Different Fractionation Methods vol.17, pp.2, 2014, https://doi.org/10.5657/FAS.2014.0181
  7. Lowering the Bitterness of Enzymatic Hydrolysate Using Aminopeptidase-active Fractions from the Common Squid (Todarodes pacificus) Hepatopancreas vol.46, pp.6, 2014, https://doi.org/10.9721/KJFST.2014.46.6.716
  8. 발효촉진제를 첨가하여 제조한 오징어 조미료의 품질특성 vol.43, pp.3, 2008, https://doi.org/10.9721/kjfst.2011.43.3.334