Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

The effect of human demineralized freeze-dried xenograft on vertical bone formation in beagle dogs

탈회동결건조골이 수직골 형성에 미치는 영향

  • Park, Ju-Hee (Department of Periodontology, School of Dentistry, Kyung Hee University) ;
  • Kwon, Young-Hyuk (Department of Periodontology, School of Dentistry, Kyung Hee University) ;
  • Park, Joon-Bong (Department of Periodontology, School of Dentistry, Kyung Hee University) ;
  • Chung, Jong-Hyuk (Department of Periodontology, School of Dentistry, Kyung Hee University) ;
  • Shin, Seung-Il (Department of Periodontology, School of Dentistry, Kyung Hee University) ;
  • Herr, Yeek (Department of Periodontology, School of Dentistry, Kyung Hee University)
  • 박주희 (경희대학교 치의학전문대학원 치주과학교실) ;
  • 권영혁 (경희대학교 치의학전문대학원 치주과학교실) ;
  • 박준봉 (경희대학교 치의학전문대학원 치주과학교실) ;
  • 정종혁 (경희대학교 치의학전문대학원 치주과학교실) ;
  • 신승일 (경희대학교 치의학전문대학원 치주과학교실) ;
  • 허익 (경희대학교 치의학전문대학원 치주과학교실)
  • Published : 2008.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: The purpose of this study was to evaluate exophytically vertical bone formation in the mandibular premolar area of beagle dogs by the concept of guided bone regeneration with a titanium reinforced e-PTFE membrane combined with human demineralized freeze-dried bone. Materials and Methods: Four one-year old beagle dogs were divided into control and experimental group. All mandibular premolars were extracted and surgical vertical defects of 5 mm in height were created in the extracted sockets. At 8 weeks after the extraction, TR e-PTFE membrane sized with 8 mm in length, 5 mm in width, and 4 mm in height was placed on the decorticated mandible, fixed with metal pins and covered with full-thickness flap and assigned as control group. In experimental group, decorticated mandibule was treated with TR e-PTFE membrane and human demineralized freeze-dried bone. The animals were sacrificed at 16 weeks after the regenerative surgery, and new bone formation was assessed by histomorphometric as well as statistical analysis. Results: Average of new bone formation was 38% in the control group, whereas was 25% in the experimental group (p<0.05). Average of connective tissue formation was 42% in the experimental group, whereas was 30% in the control group (p<0.05). The lamellar bone formation with haversian canals was observed in the both groups. In the experimental group, the particles of human demineralized freeze-dried bone were observed after 16 weeks and complete resorption of graft was not observed. Conclusion: On the basis of these findings, we conclude that titanium reinforced e-PTFE membrane may be used alone for vertical guided bone regeneration, but demineralized freeze-dried bone has no additional effect on vertical guided bone regeneration.

Keywords

References

  1. Albrektsson T, Dahl E, Embolm L. Osseointegrated oral implants. A Swedish multicenter study of 8139 consecutively inserted Nobelpharma implants. J Periodontol 1998: 59:287-296 https://doi.org/10.1902/jop.1988.59.5.287
  2. Tallgren A. The continuing reduction of the residual ridges in complete denture wearers: A mixed-longitudinal study covering 25 years. J Prosthet Dent 1972;27:120-132 https://doi.org/10.1016/0022-3913(72)90188-6
  3. Dahlin C, Linde A, Gottlow J, Nyman S. Healing of bone defects by guided tissue regeneration. Plastic Reconstr Surg 1988;81:672 https://doi.org/10.1097/00006534-198805000-00004
  4. Buser D, Dula K, Belser U, Hirt HP, Berthold H. Localized ridge augmentation using guided bone regeneration. I. Surgical procedure in the maxilla. Int J Periodont Rest Dent 1993;13:29-45
  5. Schmid J. Supraplant a novel implant system based on the principle of guided bone regeneration. Clin Oral Impl Res 1991;2:199-202 https://doi.org/10.1034/j.1600-0501.1991.020407.x
  6. Jovanovic SA, Schenk RK, Orsini M, Kenney Eb. Supracrestal bone formation around dental implants: An experimental dog study. Int J Oral Maxillofac Impl 1995; 10:23-31
  7. Simion M, Trisi P, Piattelli A. Vertical ridge augmentation using a membrane technique associated with osseointegrated implants. Int J Periodont Rest Dent 1994;14:497-511
  8. Simion M, Dahlin C, Rocchietta I. Vertical ridge augmentation with guided bone regeneration in association with dental implants: an experimental study in dogs. Clin Oral Impl Res 2007;18:86-94
  9. Hammerle CH, Jung R.E. Bone augmentation by means of barrier membranes. Peiodontology 2000 2003;33:36-53 https://doi.org/10.1046/j.0906-6713.2003.03304.x
  10. Tinti C, Parma-Benfenati S. Polizzi. Vertical augmentation: what is the limit? Int J Periodont Rest Dent 1996;16: 220-229
  11. Simion M, Jovonavic S.A, Trisi P, Scarano A, Piattelli A. Vertical ridge augmentation around dental implants using a membrane technique and autogenous bone or allografts in humans. Int J Periodont Rest Dent 1998;18:8-23
  12. Simion M, Jovonavic S.A, Tinti C, Parma-Benfenati S.P. Long-term evaluation of osseointegrated implants inserted at the time or after vertical ridge augmentation. A retrospective study on 123 implants with 1-5 year follow-up. Clin Oral Impl Res 2001;12:35-45 https://doi.org/10.1034/j.1600-0501.2001.012001035.x
  13. Schmid J, Hammerle C.H, Fluckiger L et al. Blood-filled spaces with and without filler materials in guided bone regeneration. A comparative experimental study in the rabbit using bioresorbable membranes. Clin Oral Impl Res 1997;8:75-81 https://doi.org/10.1034/j.1600-0501.1997.080201.x
  14. Bradley S, McAllister Kamran. AAP-Commissioned Review. Bone augmentation technique J Periodontol 2007: 78:377-396 https://doi.org/10.1902/jop.2007.060048
  15. Ellis E. Biology of bone grafting: An overview. Oral Maxillofac Surg 1993;2:1-15
  16. Nevins M, Mellonig JT. Enhancement of the damaged edentulous ridge to receive dental implants: A combination of allograft & the GORE-TEX Membrane. Int J Periodont Rest Dent 1992;12:97-111
  17. Fugazzotto PA. Report of 302 consecutive ridge augmentation procedures: Technical consideration & cinical results, Int J Periodont Rest Dent 1998;13:358-368
  18. Urist MR. Bone Formation by autoinduction. Science 1965;150:893-899 https://doi.org/10.1126/science.150.3698.893
  19. Urist MR. Dowel TA, Hay PH, Strates BS. Inductive substrates for bone formation. Lin Orthop 1968;59:59-96
  20. Mellonig JT. Decalcified freeze-dried bone allograft as an material in human periodontal defect. Int J Periodont Rest Dent 1984;4:40-55
  21. Sassard WR, Eidman DK, Gray PMJ. Analysis of spine fusion utilizing demineralized bone matrix. Presented at Western Orthopedic Association Meeeting, August 1994, Philadelphia
  22. Lundgren D, Lundgren AK, Sennerby L, Nyman S. Augmentation of intramembrane pus bone beyond the skeletal envelope using an occlusive titanium barrier. An experimental study in the rabbit. Clin Oral Impl Res 1995; 6:67-72 https://doi.org/10.1034/j.1600-0501.1995.060201.x
  23. Schmid J, Hammerle CH, Olah AJ, Lang NP. Membrane permeability is unnecessary for guided regeneration of new bone. An experimental study in the rabbit. Clin Oral Impl Res 1994;5:125-130 https://doi.org/10.1034/j.1600-0501.1994.050302.x
  24. von Steenberghe D, Johansson C, Quirynen M et al. Bone augmentation by means of a stiff occlusive titanium barrier. A study in rabbits and humans. Clin Oral Impl Res 2003; 14:63-71 https://doi.org/10.1034/j.1600-0501.2003.140109.x
  25. Pinholt EM, Haanaes HR, Donath K, Bang G. Titanium implant insertion into dog alveolar ridges augmented by allogenic material. Clin Oral Impl Res 1994;5:213-219 https://doi.org/10.1034/j.1600-0501.1994.050404.x
  26. Becker W, Lekholm U, Dahlin C et al. Immediate placement of titanium implants into fresh extraction sockets protected by e-PTFE membrane barriers. A clinical multicenter study. Int J Oral Maxillofac Impl 1994;9:31-40
  27. Becker W, Schenk R, Higuchi K, Lekholm U, Becker BE. Variations in bone regeneration adjacent to implants augmented with barrier membranes alone or with demineralized freeze-dried bone or autologous grafts: a study in dog. Int J Oral Maxillofac Impl 1995:10:143-154
  28. Schwartz Z, Mellonig JT, Carnes DR et al. Ability of commercial demineralized freeze-dried bone allograft to induce new bone formation. J Periodontol 1996:67:918-926 https://doi.org/10.1902/jop.1996.67.9.918
  29. Sato K, Urist MR. Induced regeneration of calvaria by bone morphogenetic protein in dogs. Clin Orthop 1985:197: 301-311
  30. Mylon Nevins, James T. Mellonig. Enhancement of the Damaged Edentulous Ridge to Receive Dental Implants: A Combination of Allograft & the GORE-TEX Membrane. Int J Periodont Rest Dent 1992;12:97-111
  31. Dahlin C, Simion M, Nanmark U, Sennerby L. Histological morphology of the e-PTFE/tissue interface in humans subjected to guided bone regeneration in conjunction with oral implant treatment. Clin Oral Impl Res 1998;9:100-106 https://doi.org/10.1034/j.1600-0501.1998.090205.x
  32. Okazaki K, Shimizu Y, Xu H, Ooya K. Blood-filled spaces with and without deproteinized bone grafts in guided bone regeneration. A histomorphometric study of the rabbit skull using non-resorbable membrane. Clin Oral Impl Res 2005; 16:236-243 https://doi.org/10.1111/j.1600-0501.2004.01095.x
  33. Hammerle C.H.F, Olah A.J, Schmid J et al. The biological effect of natural bone mineral on bone neoformation on the rabbit skull. Clin Oral Impl Res 1997:8:198-207 https://doi.org/10.1034/j.1600-0501.1997.080306.x
  34. Kostopoulos, L, Lioubavina N, Karring T, Uraguchi R. Role of chitin beads in the formation of jaw bone by guided tissue regeneration. An experiment in the rat. Clin Oral Impl Res 2001:12:325-331 https://doi.org/10.1034/j.1600-0501.2001.012004325.x
  35. Avinoam Yaffe et al. Regional Accelerated Phenomenon in the Mandibule Following Mucoperiosteal flap surgery J Periodontol 1994:65:79-83 https://doi.org/10.1902/jop.1994.65.1.79
  36. Rompen EH, Biewer R, Vanheusden A, Zahedi S, Nusgens B. The influence of cortical perforations and of space filling with peripheral blood on the kinetics of guided bone generation. A comparative histometric study in the rat. Clin Oral Impl Res 1999;10:85-94 https://doi.org/10.1034/j.1600-0501.1999.100202.x
  37. Zeina Majzoub et al. Role of intramarrow penetration in osseous repair: A Pilot study in the rabbit calvaria. J Periodontol 1999:70:1501-1510 https://doi.org/10.1902/jop.1999.70.12.1501