한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제23권2호
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  • Pages.93-100
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  • 2008
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지

Effect of Porcine Serum as Macromolecule on the Meiotic Maturation and Embryonic Development of Porcine Oocytes

  • Son, Jong-Min (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Doo-Soo (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Eon-Song (School of Veterinary Medicine, Kangwon National University) ;
  • Cho, Jong-Ki (College of Veterinary Medicine, Chungnam National University) ;
  • Shin, Sang-Tae (College of Veterinary Medicine, Chungnam National University)
  • 발행 : 2008.06.30
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초록

This study was conducted to establish an in vitro maturation (IVM) system by selection of efficient macromolecule in the porcine in vitro production (IVP) technology. To choose the efficient macromolecules in the development of porcine embryos, the effects of 3 kinds of macromolecules (porcine serum; PS, porcine follicular fluid; pFF, and polyvinyl alcohol; PVA) supplemented in IVM media on the maturation, cleavage, and development rates to blastocyst of parthenogenetic activation (PA) and in vitro fertilization (IVF) embryos were examined. The maturation rates of porcine oocytes in media supplemented with PS were significantly higher than those with pFF and PVA (92.4% vs. 85.4%, 77.1%; p<0.05). In the cleavage and development to blastocyst rates, supplement with PS or pFF in the IVM media was more effective than PA. However, there were no significant differences in cleavage and development to blastocyst between PS and pFF group. From the results of this study, it was demonstrated that PS was optimal macromolecule in the porcine IVM media.

키워드

참고문헌

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