감마선 조사한 수산 자숙액 에탄올 추출물의 유전독성학적 안전성 평가

Genotoxicological Safety of the Ethanol Extract from Seafood Cooking Drips by Gamma Irradiation

  • 김현주 (한국원자력연구원 방사선과학연구소) ;
  • 최종일 (한국원자력연구원 방사선과학연구소) ;
  • 이희섭 (한국원자력연구원 방사선과학연구소) ;
  • 김재훈 (한국원자력연구원 방사선과학연구소) ;
  • 변명우 (한국원자력연구원 방사선과학연구소) ;
  • 전병수 (부경대학교 식품생명공학부) ;
  • 안동현 (부경대학교 식품생명공학부) ;
  • 육홍선 (충남대학교 식품영양학과) ;
  • 김기혁 (우송대학교 식품영양.식품과학부) ;
  • 이주운 (한국원자력연구원 방사선과학연구소)
  • Kim, Hyun-Joo (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Choi, Jong-il (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Hee-Sub (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jae-Hun (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Byun, Myung-Woo (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Chun, Byung-Soo (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Ahn, Dong-Hyun (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Yook, Hong-Sun (Department of Food and Nutrition, Chungnam National University) ;
  • Kim, Keehyuk (Department of Food Nutrion and Food Science, Woosong University) ;
  • Lee, Ju-Woon (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 투고 : 2008.01.04
  • 심사 : 2008.01.24
  • 발행 : 2008.03.31

초록

수산가공 폐자원인 수산 자숙액의 산업적 활용 극대화 방안의 연구 일환으로 톳, 문어 및 참지 자숙액을 에탄올로 추출한 후 감마선 조사를 하여 이에 대한 유전 독성학적 안전성 평가를 하였다. 감마선 조사한 톳, 문어 및 참치 자숙액 에탄올 추출물의 S. typhimurium TA98 및 TA100에 대한 복귀돌연변이 집락수를 조사한 결과 대사활성계 도입 및 부재시 모두 시험적용농도인 $1,250{\sim}5,000{\mu}g\;plate^{-1}$의 범위에서 돌연변이가 일어나지 않았으며, E. coli PQ37에 대한 돌연변이원성을 알아본 결과 $1,250{\sim}5,000mg\;assay^{-1}$의 범위 안에서 감마선 조사에 의한 돌연변이원이 관찰되지 않았다. 따라서 감마선 조사한 톳, 문어 및 참치 자숙액은 독성을 일으키지 않고, 식품 및 공중보건산업의 소재로서 유용하게 사용할 수 있다고 판단된다.

Although seafood cooking drips were the byproducts from the fishery industry it was known that the cooking drips had many nutrients and could be used as functional materials. Previously, the physiological properties of cooking drips were shown to be increased by a gamma irradiation. But, there was no report on the safe for the genotoxicity on the irradiation. In this study, the genotoxicity of the cooking drips from Hizikia fusiformis, Enteroctopus dofleni and Thunnus thynnus was evaluated by the Ames test (Salmonella typhimurium reversion assay) and the SOS chromotest. The results from all samples were negative in the bacterial reversion assay with S. typhimurium TA98, TA100. No mutagenicity was detected in the assay, both with and without metabolic activation. The SOS chromotest also indicated that the gamma-irradiated seafood cooking drips did not show any mutagenicity. Therefore, this study indicated that gamma irradiation could be used for the hygiene, functional properties and processibility of seafood cooking drips.

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