Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Preparation of Nano-liposome by Sonication and Pressure

초음파와 압력을 이용한 나노 리포솜의 제조

  • Published : 2008.02.28
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Abstract

Liposomes are artificial membranes prepared by phospholipid. In this study, liposomes were prepared by dehydration-rehydration method, and then nano-sized by sonication and pressure. The sizes of the prepared multilamellar vesicles (MLV) were greater than 10 μm. Sonication with a tip-type sonifier or pressurization with a French press on MLV were carried out to reduce size. Sonication with an output of 112.5 W for 10 min on MLV resulted in sizes less than 450 nm. French press with 6,000 psi of pressure was able to manufacture liposomes of approximately 100 nm uniformly. Also, the sonication or pressure clarified the color of the liposome solutions. The results indicate that sonication and pressure via French press can be applied to obtain nano-sized liposomes.

리포솜은 인지질로 구성된 인위적 세포막으로서, 본 연구에서는 초음파와 압력을 이용하여 나노 리포솜을 제조하였다. 먼저 리포솜을 탈수/재수화법으로 제조하였다. 형성된 multilayer vesilces(MLV)의 크기는 $10{\mu}m$ 이상이었다. Tip-type의 초음파 처리기와 French press를 이용하여 리포솜의 크기를 감소시켰다. MLV에 대한 출력 112.5W, 10분간의 초음파 처리로 리포솜의 크기가 450 nm 이하로 감소되었다. 또한, 6,000 psi 이상의 압력에서는 100 nm 내외의 균일한 리포솜이 생성되었다. 초음파와 가압에 의한 리포솜 용액의 색도는 대체로 명도, 적색도, 황색도가증가하였으며, 육안으로는 점점 더 투명하게 되었다. 이러한 결과는 초음파와 French press를 이용한 가압 처리로 나노 크기의 리포솜을 얻을 수 있음을 의미한다.

Keywords

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