생명과학회지 (Journal of Life Science)

  • 제18권7호
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  • Pages.1015-1018
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  • 2008
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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가막산 참굴의 집단 구조 분석

The Population Genetic Structure of the Oyster Crassostera gigas (Bivalvia:Ostreidae) from Gamak Bay in Korea

  • 조은섭 (국립수산과학원 남해수산연구소) ;
  • 정희동 (국립수산과학원 남해수산연구소)
  • 발행 : 2008.07.30
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초록

본 연구는 2007년 3월 가막만에서 양식하고 있는 참굴 34마리를 대상으로 미토콘드리아COI gene 염기서열분석을 통하여 유전자 집단을 조사했다. PCR 증폭에 사용된 primer는 HCO2918과 LCO1491로 product는 658 bp였다. 유전자 배열 결과 가막만 참굴 집단에서 CR1, CR2, CR3, CR4 4개의 haplotype이 나타났다. 그 중 CR3 haplotype이 73% 발생되어 가장 높은 빈도율을 보였다. 계층구조에서도 가막산 참굴은 남해안산과 유전적 유연관계를 보이나, 유전적 거리는 남해안 및 서해안과도 마이너스 값을 보이며 통계적으로도 유전적으로 하나의 집단을 형성하고 있음을 알 수 있다.

To analyze the population genetic structure of the oyster Crassostrea gigas Thunberg, 34 specimens werecollected from Gamak bay in March, 2007. Total genomic DNA was extracted from each sample and PCR was performed to identify haplotypes of oyster by using HCO2918 and LCO1491 primers. Four kinds of haplotypes (CR1, CR2, CR3, and CR4) were identified. Among these group, CR3 showed the highest relative frequency at 73% than any other of haplotypes. On the basis of hierarchical genetic structure, the population of Gamak showed a higher genetic relationship with Namhae, but the genetic distance between southern and western coasts was negative and no statistical significance was found (p>0.05). Consequently, the oyster from Korea coast is determined to be both homogenous and large.

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참고문헌

  1. An, H. S., Y. J. Jee, D. W. Park, H. Y. Ryu and K. S. Min. 2000. Genetic variation in populations of the Pacific oyster (Crassostrea gigas), based on the mitochondrial COI gene sequence. Kor. J. Genetics 22, 249-255.
  2. Asahida, T., T. Kobayashi, K. Saitohand I. Nakayama. 1996. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea. Fish. Sci. 62, 727-730. https://doi.org/10.2331/suisan.62.727
  3. Bierne, N., S. Launey, Y. Naciri-Graven and F. Bonhomme. 1998. Early effects of inbreeding as revealed by microsatellite analyses on Ostrea edulis larvae. Genetics 143, 1893-1906.
  4. Brown, B. L. and K. T. Paynter. 1991. Mitochondrial DNA analysis of native and selectively inbred Chesapeake bay oysters, Crassostrea virginica. Mar. Biol. 110, 343-352. https://doi.org/10.1007/BF01344353
  5. Excoffier, L., P. E. Smouse and J. M. Quattro. 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131, 479-491.
  6. Hedgecock, D., D. J. McGoldrick, D. T. Manahan, J. Vavra, N. Appelmans and B. L. Bayne. 1996. Quantitative and molecular genetic analyses of heterosis in bivalve mollusks. J. Exp. Mar. Biol. Ecol. 203, 49-59. https://doi.org/10.1016/0022-0981(96)02569-5
  7. Holsinger, K. E. and R. J. Mason-Gamer. 1996. Hierarchical analysis of nucleotide diversity in geographically structured populations. Genetics 142, 629-639.
  8. Hu, Y. P. and D. W. Foltz. 1996. Genetics of scnDNA polymorphisms in juvenile oysters, Crassostrea virginica: characterizing the inheritance of polymorphisms in controlled crosses. Mol. Mar. Biol. Biotechnol. 5, 123-129.
  9. Huvet, A., P. Boudry, M. Ohresser, C. Delsert and F. Bonhomme. 2000. Variable microsatellites in the Pacific oyster Crassostrea gigas and other cupped oyster species. Anim. Genet. 31, 71-72.
  10. Kim, S. H., M. S. Park, Y. H. Kim and D. W. Park. 1997. Genetic analysis of mitochondrial DNA from Korea oysters, Crassostera gigas. J. Kor. Fish Soc. 30, 804-808.
  11. Liu, L., L. D. W. Foltz and W. B. Stickle. 1991. Genetic population structure of the southern oyster drill Stramonita (=Thais) haemostoma. Mar. Biol. 111, 71-79. https://doi.org/10.1007/BF01986348
  12. Park, D. W. 2004. Genetic variation of Korean oyster species and sterol composition of phytoplankton as oyster food. Ph.D. thesis. Busan National University, Busan.
  13. Park, M. S. and S. H. Kim. 1995. Mitochondrial DNA variation in oyster (Crassostrea gigas Thunberg and C. nippona Seki) populations from Korea and Japan. Kor. J. Syst. Zool. 11, 235-242.
  14. Schneider, S., J. M. Kueffer, D. Roessli and L. Excoffier. 1996. Arlequin: a software package for population genetics. Genetics and Biometry Lab., Department of Anthropology, University of Geneva.
  15. Seed, R. 1969. The ecology of Mytilus edulis L. (Lamellibrachiata) on exposed rocky shores. I. Breeding and settlement. Occologia 3, 277-316. https://doi.org/10.1007/BF00390380
  16. Sekino, M., M. Hamaguchi, F. Aranishi and K. Okoshi. 2003. Development of novel microsatellite DNA markers from the Pacific oyster Crassostrea gigas. Mar. Biiotechnol. 5, 227-233. https://doi.org/10.1007/s10126-002-0104-z
  17. Thomson, J. D., D. G. Higgins and T. J. Gibson. 1994. Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position- specific gap penalities and weight matrix choice. Nucleic Acids Res. 22, 4673-4680. https://doi.org/10.1093/nar/22.22.4673
  18. Yoo, S. K. 2000. A shallow sea culture. Kudeok Publishing, Busan, 605 p.