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적송잎 추출물의 proanthocyanidin 함량 분석 및 항산화 활성 검증을 통한 추출공정 확립

Establishment of the Extraction Process by Evaluation of Proanthocyanidin Contents and Antioxidative Activities of Pine (Pinus densiflora) Needle Extracts.

  • 발행 : 2008.07.30

초록

본 연구에서는 기능성 솔잎추출제품 제조를 위한 보다 효율적인 추출공정 확립을 위하여 두 가지 추출법을 조합한 추출법에 의해 얻어진 솔잎 추출물들의 proanthocyanidin 성분, 총 polyphenolic 함량 및 이들을 항산화 활성을 측정하였고 기존의 단독 추출법에 의한 추출물들과의 비교를 행하였다. 아임계 열수추출 침전물(SSHP)과 아임계 열수추출 상층액(SSHS)의 총 폴리페놀성 성분의 농도는 각각 225.4 및 116.6mg/g으로 타 추출물들에 비하여 높은 농도를 보였으며, proanthocyanidin농도 또한 각각 9.9 and 3.6 mg/g으로 비교적 높게 나타났다. 또한, 적송잎 추출물에 포함된 총 폴리폐놀성 성분에 대한 proanthocyanidin의 함량을 계산한 결과, 아임계 열수추출 침전물(SSHP) 및 아임계 열수추출 상층액(SSHS)의 경우 각각 4.3%와 3.9%로 열수 추출물(3.6%)보다 높은 proanthocyanidin 함량을 보였다. DPPH radical scavenging으로 아임계 열수추출 침전물(SSHP) 및 아임계 열수추출 상층액(SSHS)의 항산화 활성($IC_{50}$)을 측정한 결과 각각 41.5 및 $50.7\;{\mu}g/m$로 타 추출물들에 비하여 높은 활성을 나타냈다. 또한, tiocyanate법과 TBA법으로 측정한 linolic acid system에 의한 항산화 활성의 경우도 아임계 열수추출 상층액(SSHS)과 아임계 열수추출 침전물(SSHP)에서 높은 활성이 나타났다. 결론적으로, 두 가지 추출법을 조합한 추출물인 아임계 열수추출 상층액(SSHS)과 아임계 열수추출 침전물(SSHP)은 단독 추출법에 의한 추출물들 보다 높은 항산화 활성을 보여준다는 사실을 확인할 수 있었고, 이러한 추출공정은 식품소재로부터 기능성 성분을 보다 효과적으로 추출할 수 있는 방법으로 활용될 수 있을 것으로 기대된다.

We evaluated proanthocyanidin contents out of total polyphenolic compounds in two pine needle extracts prepared by combined extraction methods and compared their antioxidative activities. Amounts of total polyphenolic compounds of sub-supercritical hotwater precipitate (SSHP) and sub-supercritical hotwater supermatant (SSHS) were 225.4 and 116.6 mg/g, respectively. Amounts of proanthocyanidin of SSHP and SSHS were 9.9 and 3.6 mg/g, respectively. Thus, the ratio of proanthocyanidin contents over total polyphenolic compounds of SSHP and SSHS were 4.3 and 3.9%, respectively. Antioxidative activities ($IC_{50}$) of SSHP and SSHS measured by DPPH radical scavenging were 41.5 and $50.7\;{\mu}g/ml$, respectively. These results indicates that two combined extracts, SSHP and SSHS showed higher antioxidative activities than extract prepared by hot water or sub-supercritical. In conclusion, extraction procedures for SSHP and SSHS could be useful methods for preparing the effective components of functional food products.

키워드

참고문헌

  1. Aisling, A. and N. M. O'Brien. 2002. Dietary flvonols. Chemistry, food content and metabolism. Nutrition 18, 75-81. https://doi.org/10.1016/S0899-9007(01)00695-5
  2. Bagchi, D. A., R. L. Garg, M. Krohn, D. J. Bagchi, J. Balmoori and S. J. Stohs. 1998. Protective Effects of Grape Seed Proanthocyanidins and Selected Antioxidants against TPA-Induced Hepatic and Brain Lipid Peroxidation and DNA Fragmentation, and Peritoneal Macrophage Activation in Mice. Gen. Pharmacol. 30, 771-776. https://doi.org/10.1016/S0306-3623(97)00332-7
  3. Choi, U., D. H. Shin, Y. S. Chang and J. I. Shin. 1992. Screening of natural antioxidant from plant and antioxidative effect. Kor. J. Food Sci. Technol. 24, 149-153.
  4. Fine, A. M. 2000. Oligomeric proanthocyanidin complexs: History, structure, and phytopharmaceutical application. Altern. Med. Rev. 5, 114-151.
  5. Hong, K. S. and J. O. Sung. 1996. Isolation and structure elucidation of proanthocyanidin in bark of Pinus densiflora. Mokchae Konghak 24, 81-93.
  6. Hsu, T., S. Sheu, E. Liaw, T. Wang and C. Lin. 2005. Anti-oxidant activity and effect of Pinus morrisonicola Hay. on the survival of leukemia cell line U937. Phytomedicine 12, 663-669. https://doi.org/10.1016/j.phymed.2004.03.013
  7. Kim, A. Y. and S. C. Kang. 2007. Quality Evaluation by the Addition of Pine Needle and Artemisia princeps Extracts in Vinegared Kochujang. J. Korea Soc. Appl. Biol. Chem. 50, 167-177.
  8. Kang, Y. H., Y. K. Park, T. Y. Ha and K. D. Moon. 1996. Effects of pine needle extracts on enzyme activities of serum and liver, and liver morphology in rats fed high fat diet. J. Kor. Soc. Food Nutr. 25, 374-378
  9. Kang, Y. H., Y. K. Park., T. Y. Ha and K. D. Moon. 1996. Effects of pine needle extracts on serum and liver lipid contents in rats fed high fat diet. J. Kor. Soc. Food Nutr. 25, 367-373.
  10. Kim, J. D., T. H. Yoon, M. I. Choe, K. J. Ju and S. Y. Lee. 1991. Effect of dietary supplementation with pine lief on lipid parameters in rats. Kor. J. Gerontol. 1, 47-50.
  11. Korean Translation Committee of Dongeuibogam, 1981.
  12. Kuk, J. H., S. J. Ma and K. H. Park. 1997. Isolation and characterization of cinnamic acid with antimicrobial activity from needle of Pinus densiflora. Korean J. Food Sci. Technol. 29, 823-826.
  13. Lee, O. H., K. Y. Kim, M. K. Jang, K. H. Yu, S. G. Kim, M. Kim and S. H. Lee. 2008. Evaluation of proanthocyanidin contents on total polyphenolic compounds of pine (Pinus densiflora) needle extracts and their Antioxidative Activities. K. J. Life Sci. 18, 213-219. https://doi.org/10.5352/JLS.2008.18.2.213
  14. Lee, Y. H., Y. M. Shin, S. H. Cha, Y. S. Choi and S. Y. Lee. 1996. Development of the health foods containing the extract from Pinus strous leave. J. Kor. Soc. Food Nutr. 25, 379-383.
  15. Lee, Y. H., Y. S. Choi and S. Y. Lee. 1996. The cholesterol- lowering effects of the extract from Pinus strobus in chickens. J. Kor. Soc. Food Nutr. 25, 188-192.
  16. Liu, X., M. Dong, X. Chen, M. Jiang, X. Lv and G. Yan. 2007. Antioxidant activity and phenolics of an endophytic Xylaria sp. from Ginkgo biloba. Food Chem. 105, 548-554. https://doi.org/10.1016/j.foodchem.2007.04.008
  17. Masaki, H., S. Sakaki, T. Atsumi and H. Sakurai. 1995. Active-oxygen scavenging activity of plant extract. Biol. Pharm. Bull. 18, 162-166. https://doi.org/10.1248/bpb.18.162
  18. National Statistical Office (Republic of Korea). 1996. The expectancy of future population.
  19. Osawa, T. 1981. A novel type of antioxidant isolated from leaf was of Eucalyptus leaves. Agic. Biol. Chem. 45, 735-739. https://doi.org/10.1271/bbb1961.45.735
  20. Ottolenghi, A. 1959. Interaction of ascorbic acid and mitochondrial lipids. Arch. Biochem. Biophys. 79, 355-461. https://doi.org/10.1016/0003-9861(59)90414-X
  21. Park, G. Y., H. S. Lee, I. D. Hwang, H. S. Jung. 2006. The functional effects of fermented pine needle extract. K. J. Bio. Bioeng. 21, 376-383.
  22. Shiow, Y. and J. Ballington. 2007. Free radical scavenging capacity and antioxidant enzyme activity in deerberry. LWT - Food Sci. Technol. 40, 1352-1361. https://doi.org/10.1016/j.lwt.2006.09.005
  23. Vivas, N., M. Nonier, I. Pianet, N. Vivas de Gaulejac and E. Fouquet. 2006. Proanthocyanidins from Quercus petraea and Q. robur heartwood: quantification and structures. Comptes Rendus Chimie. 9, 120-126. https://doi.org/10.1016/j.crci.2005.09.001
  24. Yoo, J. H., J. Y. Cha, Y. K. Jeong, K. T. Chung and Y. S. Cho. 2004. Antioxidative effects of pine (Pinus denstifora) needle extracts. J. Life Sci. 14, 863-867. https://doi.org/10.5352/JLS.2004.14.5.863