Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Responses of Plasma IGF-1, IGFBPs and Hepatic GH Receptor to Growth Hormone Releasing Peptides (GHRP)-2 Administration and Energy Level in Wethers

거세면양에 있어서 에너지수준에 GHRP-2의 투여가 혈장 IGF-1, IGFBPs 및 hepatic GH 수용체에 미치는 반응

  • Lee, Hong-Gu (Department of Bio-Resources and Applied Life Science, Pusan National University) ;
  • Jin, Young-Cheng (Department of Agricultural Biotechnology, Seoul National University) ;
  • Hidari, Hisashi (Obihiro University of Agriculture and Veterinary Medicine) ;
  • Choi, Yun-Jaie (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Seon-Ku (Department of Bio-Resources and Applied Life Science, Pusan National University) ;
  • Shin, Teak-Soon (Department of Bio-Resources and Applied Life Science, Pusan National University) ;
  • Cho, Byung-Uuk (Department of Bio-Resources and Applied Life Science, Pusan National University) ;
  • Kim, Yong-Gyun (Department of Bio-Resources and Applied Life Science, Pusan National University) ;
  • Kim, Keun-Ki (Department of Bio-Resources and Applied Life Science, Pusan National University) ;
  • Son, Hong-Joo (Department of Bio-Resources and Applied Life Science, Pusan National University) ;
  • Lee, Sang-Mong (Department of Bio-Resources and Applied Life Science, Pusan National University) ;
  • Park, Hyun-Chul (Department of Bio-Resources and Applied Life Science, Pusan National University) ;
  • Kang, Han-Seok (Department of Bio-Resources and Applied Life Science, Pusan National University)
  • 이홍구 (부산대학교 생명자원과학대학 생명자원과학부 및 응용생명과학부) ;
  • 김영성 (서울대학교 농업생명과학대학 농생명공학부) ;
  • ;
  • 최윤재 (서울대학교 농업생명과학대학 농생명공학부) ;
  • 김선구 (부산대학교 생명자원과학대학 생명자원과학부 및 응용생명과학부) ;
  • 신택순 (부산대학교 생명자원과학대학 생명자원과학부 및 응용생명과학부) ;
  • 조병욱 (부산대학교 생명자원과학대학 생명자원과학부 및 응용생명과학부) ;
  • 김용균 (부산대학교 생명자원과학대학 생명자원과학부 및 응용생명과학부) ;
  • 김근기 (부산대학교 생명자원과학대학 생명자원과학부 및 응용생명과학부) ;
  • 손홍주 (부산대학교 생명자원과학대학 생명자원과학부 및 응용생명과학부) ;
  • 이상몽 (부산대학교 생명자원과학대학 생명자원과학부 및 응용생명과학부) ;
  • 박현철 (부산대학교 생명자원과학대학 생명자원과학부 및 응용생명과학부) ;
  • 강한석 (부산대학교 생명자원과학대학 생명자원과학부 및 응용생명과학부)
  • Published : 2008.07.30
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Abstract

The purpose of this study was to determine the effect of energy supplement on responses of plasma insulin-like growth factor (IGF)-1 and IGF binding proteins (IGFBPs) to growth hormone-releasing peptide-2 (GHRP-2) administration in normal protein-fed wethers, and to observe the effect of GHRP-2 treatment on hepatic growth hormone (GH) receptor in well-fed wethers. Plasma IGF-1 and 39-42 kDa IGFBP-3 during the HENP (CP, crude protein 0.34 and TDN, total digestible nutrients 1.83 kg/day DM, dry matter intake) treatment period were higher than in the LENP (CP 0.32 kg and TDN 0.87 kg/day DM intake) period (P<0.05). The response of GH was stimulated by GHRP-2 ($12.5\;{\mu}g/kg$ body weight/day) administration during both of the feed treatment periods (P<0.05). The area under curve (AUC) increment and average concentration of GH (0-180 min) with GHRP-2 administration was higher during HENP treatment than LENP treatment (P<0.01). During the HENP treatment period from day 1 to day 7 of twice daily GHRP-2 treatment, the plasma IGF-1 increment was increased on days 2, 6 and 7 of GHRP-2 administration (P<0.05). On the basis of ligand blotting, the proportions of plasma 39-43 kDa IGFBP-3 during the HENP treatment period only showed a significant difference on days 6 and 7 with GHRP-2 administration. No significant difference in the specific binding of $^{125}I-labeled$ oGH to hepatic membranes was detected between the saline and GHRP-2 treatments of the HENP-fed wethers. These results suggest that the nutritional balance between energy and protein may affect the endogenous GH / IGF-1 axis as well as plasma IGFBP-3 levels.

본연구는 정상으로 단백질을 급여한 거세면양에 있어서 에너지 첨가가 GHRP-2투여에 대한 IGF-1 및 IGFBPs에 대한 반응과, 고에너지 급여에 따른 GHRP-2투여가 hepatic GH 수용체에 미치는 영향을 검증하기 위하여 실시하였다. 시험 결과 HENP (CP 0.34 kg, TDN 1.83 kg/day DM intake)처리기간 동안 혈장 IGF-1 과 39-42kDa IGFBP-3수준은 LENP (CP 0.32 kg, TDN 0.87 kg/day DM intake)기간에 비하여 높게 나타났으나 (P<0.05), 혈장 34 kDa IGFBP-2와 24 kDa IGFBP-4는 영양처리에 의해 영향을 받지 않았다. 각 영양처리 기간동안 GHRP-2 ($12.5\;{\mu}g/kg$ body weight/day)투여는 혈장 GH 반응이 촉진되었으며(P<0.05), 혈장 GH 평균 함량과 AUC증가에 있어서는 LENP처리 기간에 비하여 HENP처리기간에서 유의적으로 높게 나타났다(P<0.01). 특히 HENP에서 7일간 GHRP-2투여에 의한 일중 혈장 IGF-1 변화양상을 조사한 결과 투여 2, 6 및 7일에서 뚜렷한 증가양상이 보였다(P<0.05). 이에 반하여 LENP에서는 오직 투여 3일째에서 Saline구에 비하여 유의적인 증가를 확인하였다(P<0.05). IGFBPs의 ligand blotting 결과 HENP구에서 혈장 39-43 kDa IGFBP-3의 수준의 증가가 투여 6일과 7일에서 관찰되었으나 혈장 IGFBP-2수준은 두 영양처리시기에서 유의적인 차이를 관찰하지 못했다. 아울러 HENP구에 있어서 간세포막에 $^{125}I-oGH$의 결합력을 측정한 결과 GHRP-2투여에 의한 영향은 관찰되지 않았다. 이와 같은 결과는 거세면양에 있어서 단백질과 에너지 사이에 영양적 균형은 내인성 GH/IGF-1 axis는 물론 혈장 IGFBP-3수준의 변화에 영향을 미치고 있음을 시사한다.

Keywords

References

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