Effects of Astragalus membranaceus Extract on Cell Cycle and Apoptosis of Human Leiomyoma Cell

황기가 in vitro에서 자궁근종세포(子宮筋腫細胞)에 미치는 영향(影響)

Purpose: This study was conducted to investigate the effects of Astragalus membranaceus extract solution on cell cycle regulation and apoptosis of human leiomyomal cell. Methods: The leiomyoma cell of patients was used in the study, and we administered the extract solution of Astragalus membranaceus concentration at 1, $10mg/m{\ell}$ to the leiomyoma cell for 48 hours. We used flow cytometry and western blotting to confirm cell cycle and apoptosis. Results: In flow cytometry, G1 phase of the $1mg/m{\ell}$ and $10mg/m{\ell}$ group was shortened and S phase of the $1mg/m{\ell}$ and $10mg/m{\ell}$ group was increased. Cycline D1 expression increased in 1, $10mg/m{\ell}$ group. Bcl-2 expression decreased in 1, $10mg/m{\ell}$ groups than control group. And Bax expression that regulated cell apoptosis more increased in 1, $10mg/m{\ell}$ group than control group. VEGF expression rised in higher Astragalus membranaceus concentration group. Conclusion: This study suggest that Astragalus membranaceus might induce cell apoptosis of leiomyoma cell and shorten cell cycle. And Astragalus membranaceus would not have the regulation effect of cell cycle.