자하거(紫河車)가 자궁근종세포(子宮筋腫細胞)의 성장억제(成長抑制)와 세포자멸사(細胞自滅死)에 미치는 영향(影響)

Effects of Hominis Placenta on the Growth of Human Uterine Myoma Cells and Cell Apoptosis

  • 위효선 (경희대학교 한방 부인과교실) ;
  • 이진무 (경희대학교 한방 부인과교실) ;
  • 이창훈 (경희대학교 한방 부인과교실) ;
  • 조정훈 (경희대학교 한방 부인과교실) ;
  • 장준복 (경희대학교 한방 부인과교실) ;
  • 이경섭 (경희대학교 한방 부인과교실)
  • 발행 : 2008.05.30

초록

Purpose: This study was conducted to investigate the effects of Hominis Placenta (紫河車) on the growth of human uterine myoma cells and cell apoptosis. Methods: Human uterine leiomyoma cells were cultured and treated with Hominis Placenta extract for 48 hours. Cell proliferation and activity was analyzed by MTT assay. We analyzed the cell cycle of human uterine myoma cells treated Hominis Placenta extract by FACS. Expression of proteins related to cell apoptosis (Bax, Bcl-2), cyclin-D1 and VEGF were evaluated by Western blotting method. Results: The human uterine myoma cells treated by Hominis Placenta extract didn't proliferate below the concentration of $10mg/m{\ell}$. And there was no remarkable difference on cell cycle analysis below the concentration of $10mg/m{\ell}$. The expression of Bax was decreased and the expression of Bcl-2 was increased after the treatment of Hominis Placenta extract. But the expressions of cyclin-D1 and VEGF were increased after the treatment of Hominis Placenta extract. Conclusion: This study suggests that Hominis Placenta induce uterine myoma cell apoptosis and have effect on the myoma cell proliferation in the concentraion below $10mg/m{\ell}$.

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