Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Effects of the Constituents of Gardenia Fructus on Prostaglandin and NO Production

  • Lim, Hyun (College of Pharmacy, Kangwon National University) ;
  • Park, Kwang-Rock (Dept. Biotechnology, Dongguk University) ;
  • Lee, Dong-Ung (Dept. Biotechnology, Dongguk University) ;
  • Kim, Yeong-Shik (Natural Products Research Institute and College of Pharmacy, Seoul National University) ;
  • Kim, Hyun-Pyo (College of Pharmacy, Kangwon National University)
  • Published : 2008.06.30
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Abstract

The fruits of Gardenia jasminoides Ellis have been previously reported to possess anti-inflammatory activity. In this study, the constituents including geniposide, geniposidic acid, genipin and crocin were evaluated for their effects on prostaglandin and NO production in an attempt to establish anti-inflammatory cellular mechanisms. Among the constituents tested, only genipin significantly inhibited cyclooxygenase-2-mediated $PGE_2$ and inducible nitric oxide synthase-mediated NO production from lipopolysaccharide-treated RAW 264.7 cells at 10-100 ${\mu}$M. Genipin also inhibited nuclear transcription factor-${\kappa}B$ activation. Moreover, genipin showed in vivo antiinflammatory activity on ${\lambda}$-carrageenan-induced paw edema in mice (10.4-29.9% inhibition at 20-100 mg/kg, i.p.). All of these results suggest that genipin may contribute to anti-inflammatory activity of the fruits of G. jasminoides and an inhibitory action on prostaglandin and NO production is, at least, the part of anti-inflammatory mechanism of genipin.

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References

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