Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Changes in the Seed Viability and DPPH-radical Scavenging of Helianthus annuus. Seeds According to Low Dose ${\gamma}$-ray irradiation

감마선 조사-해바라기 종자의 저장기간에 따른 종자활력과 추출물의 항산화활성

  • Kim, Sun-Il (Department of Chemical and Biochemical Engineering, Chosun University) ;
  • Lee, Hyun-Hwa (Department of Biology, Chosun University) ;
  • Kim, Chun-Sung (Department of Oral Physiology, Chosun University) ;
  • Seo, Myung-Deok (Department of Internal Medicine, Cheju National University Hospital) ;
  • Lee, Sook-Young (Research Center for Proteineous Materials, Chosun University)
  • 김선일 (조선대학교 생명화학공학과) ;
  • 이현화 (조선대학교 생물학과) ;
  • 김춘성 (조선대학교 구강생리학실) ;
  • 서명덕 (제주대학교 부속병원 내과) ;
  • 이숙영 (조선대학교 단백질소재연구센터)
  • Published : 2008.06.30
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Abstract

This study was to elucidate the effect of low dose gamma (${\gamma}$)-ray irradiation on the germination, seedling growth and antioxidant activity in sunflower seeds. The seed germination was stimulated in the range of 2.7${\sim}$8.0% by low dose ${\gamma}$-ray irradiation compared with that of the control; however, the germination of seeds stored for 4 months after irradiated was decreased at all low dose ${\gamma}$-ray irradiation groups. Especially, the germination rate of 32 Gy-irradiated seed group was much lower than that of the other groups and 8 Gy was the optimal radiation dose for germination at room temperature. Though there was variation with storage time and temperature, seedling growth stored at 10$^{\circ}C$ for 4 months after irradiated was promoted at the 1 Gy ${\gamma}$-ray irradiation group compared to seed stored at 25$^{\circ}C$. For the antioxidant activity of ${\gamma}$-ray irradiated seeds extract, 4 Gy and 32 Gy groups were significantly increased by 68.83% and 95.44%, respectively, compared to control at the concentration of 100 ${\mu}g$/ml.

저선량 감마선 조사에 의한 해바라기종자의 발아율과 초기생육을 통해 수량증감 여부를 조사하였고, DPPH radical 소거능 조사를 통한 항산화 활성을 분석하였다. 방사선을 조사한 직후 발아율은 무처리의 대조구에 비해 증가하였으며, 4개월 실온 보관한 후의 종자 발아율은 대조구에 비해 전체적으로 감소하는 효과를 나타내엇다. 특히 조사량이 높은 32 Gy에서의 감소율이 가장 높았으나 적정선량은 8 Gy이다. 조사된 종자의 보관 온도에 따라 초기생육에 미치는 영향을 조사한 결과, 1 Gy 조사구의 10$^{\circ}C$ 보관된 종자가 25$^{\circ}C$ 보관된 조사구에 비해 초장과 근장의 높은 증가 효과를 보여주었다. DPPH radical 소거법에 의한 항산화활성을 측정한 결과 방사선을 비조사한 대조구에 비해 조사구에서 항산화 활성이 증가하였으며, 감마선조사 후 저장기간에 관계없이 4 Gy에서 가장 높은 활성이 관찰되었다.

Keywords

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