한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제21권3호
  • /
  • Pages.184-191
  • /
  • 2008
  • /
  • 1226-3591(pISSN)
  • /
  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
권호 표지

Effects of Media Composition on Plant Regeneration and Callus Formation of Abeliophyllum distichum Nakai

  • Lee, Cheol-Hee (Dept. of Horticultural Science, Chungbuk National University) ;
  • Jin, Yeon-Hee (Dept. of Horticultural Science, Chungbuk National University) ;
  • Chang, Young-Deug (Dept. of Horticultural Science, Chungbuk National University) ;
  • Hwang, Ju-Kwang (Dept. of Horticultural Science, Chungbuk National University)
  • 발행 : 2008.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This experiments were carried out to find out the effects of different explant materials, kinds and concentration of plant growth regulators, and total nitrogen and sucrose contents on the in vitro regeneration of Abeliophyllum distichum Nakai. The effects of growth regulators on regeneration from 3 explant sources (leaf, internode and node) were more or less same. Leaf explants produced only callus with 2ip (Isopentenyladenine) and NAA (Naphthaleneacetic acid) treatment and other regulators had no effects. Test with internode explants yielded about same results but callus was obtained with 2,4-D (2,4-Dichlorophenoxyacetic acid). Node explants resulted in shoot regeneration by all regulator treatment except NAA and 2,4-D, but control also showed similar results. Callus formation from internode and node explants was vigorous by 2ip, zeatin, and 2,4-D treatments and high NAA concentration resulted in higher callus formation. In this experiment, various mixed treatment of growth regulators were also employed, using node as explant material. Shoot regeneration was obtained with BA (Benzyl adenine) + NAA treatments but the results were comparable with control. Generally shoot and root regeneration was poor with all combined treatment except 2ip + NAA and 2,4-D + NAA. However, callus was formed readily with all treatments. In this experiment, combined treatments of regulators were applied on the callus derived from singular regulator treatment. The results showed no shoot and root regeneration with any combination of 2,4-D, IAA (Indoleacetic acid) and NAA, but soft milky white callus was formed in all the treatments. No shoot and root regeneration was observed with any combination of 2iP, NAA and IAA, but somewhat hard, light green callus was formed in all the treatments. Callus formation decreased with high kinetin concentration in case of kinetin + NAA treatment. The experiments with total nitrogen content of media showed that low concentrations of 15 and 30mM were effective for the shoot and root regeneration. Sucrose experiment demonstrated shoot regeneration with 1${\sim}$4% concentration, and root and callus formation with 2${\sim}$4%. No root and callus formation was observed with 0 and 1% sucrose.

키워드

참고문헌

  1. Eom, S.H., W.H. Kang, D.Y. Shin, K. Heo and W.C. Choi. 2002. Callus formation and multiple shoot induction of Hovenia dulcis Thunb. Kor. J. Plant. Res. 15: 237-242 (in Korean)
  2. Kim, D.C., H.J. Chung, B.H. Min and D.C. Yang. 2001. Plant regeneration from leaf and internode segment cultures of Boxthorn (Lycium chinense Mill). Kor. J. Plant Tiss. Cult. 28: 329-333 (in Korean)
  3. Kim, H.K., J.C. Kim and C.D. Jin. 1997. Plant regeneration from internode explants of Actinidia arguta and its histological observation. Kor. J. Plant Tiss. Cult. 24: 263-268 (in Korean)
  4. Kim, D.K., K.R. Park and J.H. Kim. 2002. A taxonomic study of Abeliophyllum Nakai (Oleaceae) based on RAPD analysis. Kor. J. Plant Res. 15: 26-35 (in Korean)
  5. Lee. S.H., E.J. Hahn and K.Y. Paek. 2005. Effect of medium strength and plant growth regulator concentration on growth through node culture in Withania sonmifera (L.) Dunal. Kor. J. Hort. Sci. Technol. 23(Suppl. I): 137 (in Korean)
  6. Lee. S.Y., S.O. Yoo, J.H. Bae and J.H. Lee. 2002. Effect of plant growth regulators on callus induction and plant regeneration of Farfugium japonica. Kor. J. Plant Biotechnol. 29: 45-49 (in Korean) https://doi.org/10.5010/JPB.2002.29.1.045
  7. Lee, T.B. 1990. Conservation of threatened plant in Korea. Bull. Kwanak Arboretum 3: 190-196 (in Korean)
  8. Miller, C.L. 1993. Conservation of germplasm in forest trees. In: Ahuja, M.R., Libby, W.J. (eds). Clonal Forestry II: Conservation and Application, pp. 42-65. Springer-Velag, Berlin
  9. Moon, H.K., G.Y. Suk, Y.J. Kwon and S.H. Son. 1999. Micropropagation of rare species, Abeliophyllum distichum Nakai via axillary bud culture. Kor. J. Plant Tiss. Cult. 26: 133-136 (in Korean)
  10. Moon, H.K., G.Y. Suk and S.C. Kim. 1997. Micropropagation of a rare species, Forsythia saxatilis N. though tissue culture. J. Kor. For. Soc. 86: 430-434 (in Korean)
  11. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-597 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  12. Rai. V.R. and K.S.J. Chandra. 1988. In vitro regeneration of plantlets from shoot callus of mature trees of Dalbergia latifolia. Plant Cell Tiss. Org. Cult. 13: 77-83 https://doi.org/10.1007/BF00043048
  13. Seong N.S., C.H. Park, S.T. Lee and K.B. Youn. 1993. Callus induction and plant regeneration from axillary bud of Aconitum camichaeli Debx. Kor. J. Breed. 25: 222-229 (in Korean)
  14. Tao. R. and A. Sugiura. 1992. Adventitious bud formation from callus cultures of Japanese persimmon. HortScience 27: 259-261
  15. Withers, L.A. 1991. Biotechnology and plant genetic resources conservation. Reprinted from the Book of Plant Genetics Resources Conservation and Management. pp. 273-297
  16. Woo, B.M. 1990. Designation and conservation of the protected areas. Res. Bull. Seoul Nat'l Univ. For. 26: 68-86 (in Korean)
  17. Youn, Y., S.K. Lee and J.I. Park. 1992. In vitro propagation of a rare species - Berchemia berchemiaefolia. Res. Rep. For. Gen. Res. Inst. Kor. 28: 63-67 (in Korean)