Identification of Nontuberculous Mycobacteria Existing in Public Bathroom Water by PCR-Restriction Fragment Length Polymorphism

PCR-RFLP에 의한 대중목욕탕 내 Nontuberculous Mycobacteria의 동정

  • Choi, Seung-Gu (Department of Clinical Laboratory Science, Shin Heung College) ;
  • Song, Woon-Heung (Department of Clinical Laboratory Science, Shin Heung College) ;
  • Kang, Chee-Hwan (Department of Clinical Laboratory Science, Shin Heung College) ;
  • Cho, Kyu-Bong (Department of Clinical Laboratory Science, Shin Heung College) ;
  • Lee, Jae-Sang (DongAm Medical Institute) ;
  • Lee, Jang-Ho (Department Laboratory Medicine, Samsung Medical Center) ;
  • Kim, Sung-Il (Department of Laboratory medicine, Korea University Guro Hospital) ;
  • Jee, Soo-Il (Department of Pathology, National Health Insurance Coporation Ilsan Hospital)
  • Published : 2008.06.30

Abstract

Thirty two of bathroom water samples from public bathroom in Seoul areas were examined using acid-fast staining, Lowenstein-Jensen (L-J) medium culture and PCR-restriction fragment length polymorphism (PCR-RFLP). In 6.25% (2/32) bathroom water samples, acid-fast bacilli were detected by AFB stain, and in 21.9% (7/32) bathroom water samples, acid fast bacilli grew on L-J media. Of them, six acid-fast bacilli were identified as Mycobacterium avium, and the other AFB as Mycobacterium szulgai by PCR-RFLP. These results are suggested that accidental nontuberculosis mycobacterial infection to a weakness person will be possible in public area.

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