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식물플랑크톤의 개체군성장저해율을 이용한 해양생태독성 시험방법에 관한 연구

Application of the Ecotoxicological Standard Method using Population Growth Inhibition of Marine Phytoplankton

  • 이승민 (국립수산과학원 서해수산연구소) ;
  • 박경수 (안양대학교 해양생명공학과) ;
  • 안경호 (국립수산과학원 서해수산연구소) ;
  • 박승윤 (국립수산과학원 서해수산연구소) ;
  • 이상희 (한국기초과학지원연구원)
  • Lee, Sung-Min (Marine Environment Division, West Sea Fisheries Research Institute, NFRDI) ;
  • Park, Gyung-Soo (Department of Marine Biotechnology, Anyang University) ;
  • An, Kyoung-Ho (Marine Environment Division, West Sea Fisheries Research Institute, NFRDI) ;
  • Park, Soung-Yun (Marine Environment Division, West Sea Fisheries Research Institute, NFRDI) ;
  • Lee, Sang-Hee (Division of Electron Microscopic Research, Korea Basic Science Institute)
  • 발행 : 2008.05.31

초록

해양생태독성평가를 위한 공정시험방법으로 해양생태계의 기초생산자를 대표하는 식물플랑크톤인 Skeletonema costatum, Heterosigma akashiwo, Prorocentrum micans, Isochrysis galbana, Tetraselmis suecica를 이용하여 생물 검정 예비 실험을 수행하였다. 그중 표준시험생물로 해산규조류인 S. costatum을 표준시험생물종으로 선정하였으며, Endpoint는 $72{\sim}96$시간 개체군성장저해율($EC_{50}$)로 설정하였다. 시험방법은 비교환정수방식(non-renewal static test)을 선택하였으며, 시험적합도 기준은 대조구의 시간당 개체군성장률 0.04(r > 0.04/hr) 이상으로 설정하였다. S. costatum은 우리나라는 물론 전 세계적으로 널리 분포하며, 표준시험방법개발은 국제표준화기구(ISO, International Standardization Organization)의 시험방법을 참고하였다. 본 종을 이용한 독성시험은 시험대상물질의 염분이 $20{\sim}35\;psu$의 범위에서 가능하며, 반복실험 및 교차분석결과 표준독성물질에 대한 민감도가 유사하게 나타남으로써 실험의 재현성이 입증되었다. 하수오니 용출액을 이용한 독성실험 결과, 식물플랑크톤의 개체군성장저해율($EC_{50}$)을 이용한 독성실험은 윤충류(Brachionus plicatilis) 신생개체(neonate)의 사망률($LC_{50}$), 발광박테리아(Vibrio fischeri)의 발광저해율($IC_{50}$), 해조류(Ulva pertusa)의 생식률($EC_{50}$)을 이용한 시험방법보다 낮은 농도에서 $EC_{50}$을 나타냈다. 식물플랑크톤의 개체군 성장저해율을 이용한 생물검정 방법은 유해물질의 해양 기초생산자에 대한 독성평가에 매우 유용한 실험 방법으로 판단된다.

A series of experiments were conducted to establish a marine ecotoxicological standard method using marine primary producers, Skeletonema costatum, Heterosigma akashiwo, Prorocentrum micans, Isochrysis galbana, Tetraselmis suecica as candidate test species. Marine diatom, S. costatum was selected as standard test species in terms of the ecological roles and international uses as test species. Test methods and test acceptability criteria are as follows; $72{\sim}96$ hr population growth inhibition $EC_{50}$ as endpoint. Static non-renewal method, and population growth rates over 0.04/hr in control as test acceptability criterium. S. costatum is widely distributed in the world ocean and used for standard species of marine toxicity test method by ISO (International Standardization Organization). Possible salinity ranges for this test method are $20{\sim}35\;psu$, and reproducibility and interlaboratory test results were consistent through the calibration tests. Sensitivity of the test method was comparable or better than other toxicity tests such as rotifer neonate mortality, bioluminescent bacterial inhibition, seaweed sporulation and sea urchin fertilization tests.

키워드

참고문헌

  1. 박경수, 이상희, 이승민, 윤성진, 박승윤, 2005. 해양생태독성평가 를 위한 표준시험생물로서의 식물플랑크톤에 관한 연구. 한국 환경과학회지, 14: 1129−1139 https://doi.org/10.5322/JES.2005.14.12.1129
  2. 환경교육연구회, 2000. 환경오염공정시험법(폐기물 토양오염분야). 대학서림. 564pp
  3. Anderson, B.S., J.W. Hunt, B.M. Phillips, R. Fairey, C.A. Roberts, J.M. Oakden, H.M. Puckett, M. Stephenson, R.S. Tjeerdema, E.R. Long, C.J. Wilson and J.M. Lyons, 2001. Sediment quality in Los Angeles Harbor, USA: A triad assessment, Environ. Toxicol. Chem., 20: 359−370
  4. APHA, AWWA and WEF, 1995. Standard Methods for the Examination of Water and Wastewater. American Public Health Association, American Water Works Association, Water Environment Federation, Washington, DC, 1−47pp
  5. Burton, G.A., 1992. Sediemnt Toxicity Assessment. Lewis Publishers Inc., Chelsea. 457pp
  6. Chapman, P.M., 1992. Pollution status of North Sea sediments- an international integrative study. Mar. Ecol. Prog. Ser., 91: 313−322
  7. Cheung, Y.H., A. Neller, K.H. Chu, N.F.Y. Tam, C.K. Wong, Y.S. Wong and M.H. Wong, 1997. assessment of sediment toxicity using different trophic organisms. Arch. Environ. Contam. Toxicol., 32: 260−267
  8. Dunnett, C.W., 1964. New table for multiple comparisons with a control. Biometrics, 20: 482 https://doi.org/10.2307/2528490
  9. Ghirardini, A.V., T. Birkemeyer, A.A. Novelli, E. Delaney, B. Pavoni and P.F. Ghetti, 1999. An integrated approach to sediment quality assessment: the Venetian lagoon as a case study. Aquat. Ecosyst. Health Manage., 2: 435−447
  10. Hooten, R.L. and R.S. Carr, 1997. Development and application of a marine sediment pore-water toxicity test using Ulva fasciata zoospores. Environ. Toxicol. Chem., 17: 932−940
  11. ISO, 1995. Water quality- marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum. the International Organization for Standardization. ISO 10253, 7pp
  12. Jensen, A., B. Rystad and S. Melsom, 1974. Heavy metal tolerance of marine phytoplankton. 1. the tolerance of three algal species to zinc in coastal sea water. J. Exp. Mar. Biol. Ecol., 15: 145−157
  13. MOMAF, 2004. Development of Assessment Framework on the Sewage Sludge for Ocean Disposal. Ministry of Maritime Affairs and Fisheries, Korea, 390pp
  14. NIWA, 1998. Marine Algae (Dunaliella tertiolecta) Chronic Toxicity Test Protocol. National Institute of Water and Atmospheric Research, New Zealand, 30pp
  15. Overnell, J., 1976. Inhibition of marine algal photosynthesis by heavy metals. Mar. Biol., 38: 335−342
  16. Park. G.S., C.S. Chung, S.H. Lee, G.H. Hong, S.H. Kim, S.Y. Park, S.J. Yoon and S.M. Lee, 2005. Ecotoxicological evaluation of sewage sludge using bioluminescent marine bacteria and rotifer. Ocean Science Journal, 40: 91−100 https://doi.org/10.1007/BF03028589
  17. Rand, G.M. and S.R. Petrocelli, 1985. Fundamentals of Aquatic Toxicology. Hemisphere Publishing Corporation, Washington. 666pp
  18. Rao, M.U. and V. Mohanchand, 1990. Toxicity of zinc smelter wastes to some marine diatoms. Indian J. of Marine Sciences, 19: 181−186
  19. Ravail, B. and J.M. Robert, 1985. Influence of salinity on the growth of S. costatum (Greville) Cleve in the waters of the Loire Estuary. Cryptogramie: Algol., 6: 51−60
  20. Shapiro, S.S. and M.B. Wilk, 1965. An analysis of variance test for normality (complete samples). Biometrika, 52: 591−611
  21. Shimura, S., H. Shibuya and S. Ichimura, 1979. Growth and photosynthesis properties of some planktonic marine diatoms at various salinity regimes. Umi/la mer, Tokyo, 17(3): 149−155
  22. Silvia, S., A. Re, P. Pestana, A. Rodrigues and V. Quintino, 2004. Sediment disturbance off the Tagus Estuary, Western Portugal: chronic contamination, sewage outfall operation and runoff events. Mar. Pollut. Bull., 49: 154−162
  23. Snedecor, G.W. and W.G. Cochran, 1989. Statistical Methods. Eighth Edition, Iowa State University Press
  24. Walsh, G.E. and S.V. Alexander, 1980. A marine algal bioassay method: results with pesticides and industrial waste. Water Air Soil Pollut., 13: 45−55
  25. Weideborg, M., E.A. Vik, G.D. Ofjord and O. Kjonno, 1997. Comparison of three marine screening tests and four Oslo and Paris commission procedures to evaluate toxicity of offshore chemicals. Environ. Toxicol. Chem., 16: 384−389
  26. Wong, Y.S. N.F.Y. Tam, P.S. Lau and X.Z. Xue, 1995. The toxicity of marine sediments in Victoria Harbour, Hong Kong. Mar. Pollut. Bull., 31: 4−12