International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Age-Related Changes of Adult Neural Stem Cells in the MouseHippocampal Dentate Gyrus

  • Jung, Ji-Yeon (Dental Science Research Institute, Brain Korea 21 Project, School of Dentistry Chonnam National University) ;
  • Byun, Kang-Ok (Dental Science Research Institute, Brain Korea 21 Project, School of Dentistry Chonnam National University) ;
  • Jeong, Yeon-Jin (Dental Science Research Institute, Brain Korea 21 Project, School of Dentistry Chonnam National University) ;
  • Kim, Won-Jae (Dental Science Research Institute, Brain Korea 21 Project, School of Dentistry Chonnam National University)
  • Published : 2008.06.30
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Abstract

This study was designed to investigate the changes in the properties of the neuronal setm cells or progenitor cells associated with age-related decline in neurogenesis of the hippocampal dentate gyrus (DG). Active whole cells cycle marker Ki67 (a marker of whole cell cycle)-positive and S phase marker bromodeoxyuridine (BrdU)-positive. Neural stem cells gradually were reduced in the hippocampal subgranular zone (SGZ) in an age-dependant manner after birth (from P1 month to P1 year). The ratio of BrdUpositivecells/Ki67-positive cells was gradually enhanced in an age-dependent manner. The ratio of Ki67-positive cells/accu-mulating BrdU-positive cells at 3 hrs after BrdU injection was injected once a day for consecutive 5 days gradually decreased during ageing. TUNEL- and caspase 3 (apoptotic terminal caspase)-positive cells gradually decreased in the dentate SGZ during ageing and immunohistochemical findings of glial fibrillary acid protein (GFAP) were not changed during ageing. NeuN, a marker of mature neural cells, and BrdU-double positive cells gradually decreased in an age-dependent manner but differentiating ratio and survival rate of cells were not changed at 4 wks after BrdU injection once a day for consecutive 5 days. The number of BrdU-positive cells migrated from the hippocampal SGZ into granular layer and its migration speed was gradually declined during ageing. These results suggest that the adult neurogenesis in the mouse hippocampal DG gradually decrease through reducing proliferation of neural stem cells accompanying with cells cycle change and reduced cells migration rather than changes of differentiation.

Keywords

References

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