한국토양비료학회지 (Korean Journal of Soil Science and Fertilizer)

  • 제41권3호
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  • Pages.214-222
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  • 2008
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  • 0367-6315(pISSN)
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  • 2288-2162(eISSN)
한국토양비료학회 (Korean Society of Soil Science and Fertilizer)
권호 표지

전남지역 인삼재배 토양의 Arbuscular 균근균 분포 특성

Distribution of Arbuscular Mycorrhizal Fungi(AMF) at Ginseng Cultivated Fields in Jeonnam Province

  • 손보균 (순천대학교 생명과학부) ;
  • 진서영 (순천대학교 생명과학부) ;
  • 이도진 (순천대학교 농업교육과)
  • Sohn, Bo-Kyoon (Division of Environmental and Agricultural Science, Sunchon National University) ;
  • Jin, Seo-Young (Division of Environmental and Agricultural Science, Sunchon National University) ;
  • Lee, Do-Jin (Department of Agricultural Education, Sunchon National University)
  • 투고 : 2008.05.03
  • 심사 : 2008.06.14
  • 발행 : 2008.06.30
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초록

전남지역 인삼재배지의 토착 Arbuscular 균근균(AMF)의 분포와 감염특성을 파악하기 위해 곡성, 영광, 영암, 해남 지역을 대상으로 58개 지점의 토양과 인삼 시료를 채취하여 조사하고 AMF 밀도와 토양특성을 조사하였다. 조사지역의 전체 평균 포자밀도는 건토 1 g당 3.4~5.9개의 분포를 보였으며, 재배연수 3년근 재배지까지는 포자밀도가 다소 증가하는 경향을 보였고, 자연상태 토양 중의 AMF 포자밀도는 건토 1 g 당 6.3~14.7개로서 인삼재배지 보다 포자밀도가 높았다. 포자크기별 분포는 직경 $45{\sim}106{\mu}m$ 범위에 74.3% 정도가 포함되었으며, AMF의 감염 양상은 Paris-type이 주를 이루었다. 평균 감염율은 영암지역의 재배지에서 64.4%로 높게 나타났으나 그 밖의 지역은 40~50%범위를 보였다. 조사지역의 근권토양 중 AMF 외생균사의 길이는 건토 1 g당 0.5~4.4 m 범위로서 인삼의 재배연수가 증가함에 따라 외생균사(external hyphae)의 양이 많아지는 것으로 파악되었다.

This study was carried out to investigate the distribution characteristics, colonization patterns of arbuscular mycorrhizal fungi (AMF) at Ginseng- cultivated fields in Jeonnam province including Gokseong, Yeonggwang, Yeongam and Haenam area. Average density of AMF spores in all of analyzed areas was in the range of $3.4{\sim}5.9spores\;g^{-1}$ air-dried soil and spore density in the soil was increased gradually up to Ginseng cultivated fields grown for 3 years. However, AMF spore densities in the marginal field soil were $6.3{\sim}14.7spores\;g^{-1}$ air-dried soil and it was higher than those in Ginseng-cultivated field soils. A $45{\sim}106{\mu}m$ in diameter of AMF spores by size was dominant (74.3%) in Ginseng cultivated fields. AMF colonization association type in Ginseng roots was a Paris-type. Average rates of AMF infection ranged from 40% to 50%, while 64.4% of AMF infection occurred in Yeongam area. External hyphae length (EHL) in the rhizosphere soil of Ginseng cultivated field was in the range of $0.5{\sim}4.4m\;g^{-1}$ air-dried soil and the hyphae quantity was gradually increased proportional to increment of Ginseng cultivation years.

키워드

참고문헌

  1. Abbott, L.K., and A.D, Robson. 1984. The effect of VA mycorrhizae on plant growth. In:VA mycorrhiza. Ed by:C.L Powell & D.J. Baagyaraj. CRC Press. Boca Raton. p. 113-130.
  2. Armstrong, L. and R.L. Peterson. 2002. The interface between the arbuscular mycorrhizal fungus Glomus intraradices and root cells of Panax quinquefolius: a Paris-type mycorrhizal association. Mycologia 94:587-595. https://doi.org/10.2307/3761710
  3. Blal, B. 1999. Utilization of commercial arbuscular mycorrhizal fungi inoculants in ornamental and Woody plants in nursery. Acta Horticulturae 496:461-470.
  4. Brundrett, M., R.L. Peterson, L. Melville, H. Addy, T.P. McGonigle, G. Schaffer, N. Bougher, H. Massicotte. 1994. Practical methods in mycorrhiza research. mycologue Publication. p. 161.
  5. Budi. S.W., C. Cordier, A. Trouvelot, V.G. Pearson, and S. Gianinazzi. 1998. Arbuscular mycorrhiza as a way of promoting sustainable growth of micropropagated plants. Acta Horticulturae 457:71-78.
  6. Chamber, C.A., S.E. Smith, and F.A. Smith. 1980. Effects of ammonium and nitrate ions on mycorrhizal infection, nodulation and growth of Trifolium subterraneum. New Phytol. 85:47-62. https://doi.org/10.1111/j.1469-8137.1980.tb04447.x
  7. Daniels, B.A. and H.A. Skipper. 1982. Methods for the recovery and quantitative estimation of propagules from soil. Pages 29-35 in N.C. Schenck, editor. methods and principles of mycorrhizal research. American Phytopathological Society, St. Paul, Minn.
  8. Gallaud, I. 1905. Etudes sur les mycorhizes endophytes. Revue Generale de Botanique 17:5-500.
  9. Hetrick, B.A. 1986. Ecology of VA mycorrhizal fungi. In: VA Mycorrhiza (Powell, C.L., and D.J. Bagyaraj, ed.). p. 35-56. CRC Press, Boca Raton, Florida, USA.
  10. Jaizme-Vega, M. and M. Diaz-Perez. 1999. Effect of Glomus intraradices on Phoenix roebelenii during the nursery stage Acta Horticulturae(ISHS). 486: 199-202.
  11. Jakobsen, I. and L. Rosendahl. 1990. Carbon Flow into Soil and External Hyphae from Roots of Mycorrhizal Cucumber Plants. New Phytol. 115:77-83. https://doi.org/10.1111/j.1469-8137.1990.tb00924.x
  12. Jasper, D.A., A.D. Robson, and L.K. Abbott. 1979. Phosphorus and the formation of vesicular-arbuscular mycorrhizas. Soil Biology and Biochemistry 11: 501-505. https://doi.org/10.1016/0038-0717(79)90009-9
  13. Kapulnik, Y., D.D. Douds, Jr. 2000. Arbuscular Mycorrhizas: Physiology and Function. Kluwer Academic Publishers. pp. 372.
  14. Li, T.S.C. 1995. Effect of Vesicular-arbuscular mycorrhizae on the growth of american ginseng. Korean J. Ginseng 19:73-76.
  15. McGonigle, T.P., J.P. Hovius, and R.L. Peterson. 1999. Arbuscular mycorrhizae of American Ginseng(Panax quinquefolius) in cultivated field plots : Plant age affects the development of a colonization lag phase, Can. J. Bot. 77: 1028-1034. https://doi.org/10.1139/cjb-77-7-1028
  16. McNaughton, S.J., and M. Oestenheld. 1990. Extramatrical mycorrhizal abundance and grass nutrition in a tropical grazing ecosystem, the Serengeti National Park, Tanzania. Oikos, 59:92-96. https://doi.org/10.2307/3545127
  17. Miller, R.M., and J.D. Jastrow. 1992. The role of mycorrhizal fungi in soil conservation, In: G.J. Bethlenfalvay and R.G. Linderman(eds), Mycorrhiza in sustainable agriculture. ASA/CSSA/SSSA, Madison, WI. p. 29-44.
  18. Phillips, J.M., and D.S. Hayman. 1970. Improved Procedures for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Translocations of the British Mycological Society. 55 :158-160. https://doi.org/10.1016/S0007-1536(70)80110-3
  19. Ratnayake, M., R.T. Leonard, and J.A. Menge. 1978. Root exudation in relation to supply of phosphorus and its possible relevance to mycorrhizal infection. New Phytol. 81:543-552. https://doi.org/10.1111/j.1469-8137.1978.tb01627.x
  20. Rural Development Administration. 1989. Method of soil chemical analysis. RDA, Suwon, Korea.
  21. Schenck, N.C. and Y. Perez. 1990. Manual for the identification of VA mycorrhizal fungi, 3rd ed.. Gainesvile, florida, USA. p. 245.
  22. Smith, F.A., and S.E. Smith, 1996. Mutualism and parasitism : Diversity in function and structure in the "arbuscular"(VA) mycorrhizal Symbiosis. Adv Bot Res, 22:1-43. https://doi.org/10.1016/S0065-2296(08)60055-5
  23. Smith, F.A., and S.E. Smith, 1997. Tansley Review No. 96. Structural diversity in (Vesicular) arbuscular mycorrhizal symbioses. New Phytol, 137:373-388. https://doi.org/10.1046/j.1469-8137.1997.00848.x
  24. Sohn, B.K. 2000. Studies on inoculation method of indigenous arbuscular mycorrhizal fungi for improvement of Ginseng productivity. Final report. Ministry of Aagriculture & Forestry. p. 295.
  25. Sohn. B.K., H.L. Kim, and Y.J. Kim. 2003. Density of arbuscular mycorrhizal fungi and chemical properties of soil in seasoning crop cultivation. Korean J. Soil Sci. Fert., 36:145-153.
  26. Sohn, B.K., S.M. Huh, and K.Y. Kim. 1994. Effect of Inoculation with Vesicular-Arbuscular Mycorrhizal(VAM) Fungi on the Early Growth of Strawberry Plantlets. Korean J. Soil Sci. Fert., 27:54-63.
  27. Tennant, D. 1975. A test of a modified line intersection method of measuring root length. Joumal of Ecology. 63:995-1001. https://doi.org/10.2307/2258617
  28. Tisdall, J.M. and J.M. Oades. 1979. Stabilization of soil aggregates by the root systems of ryegrass. Australian Journal of Soil Research. 17:429-441. https://doi.org/10.1071/SR9790429
  29. Whitbread, F., T.P. McGonigle, and R.L. Peterson. 1996. Vesiculararbuscular mycorrhizal associations of American Ginseng(Panax quinquefolius) in commercial production. Can. J. Bot. 74:1104-1112. https://doi.org/10.1139/b96-135
  30. Wright, S.F., and A. Upadhyaya. 1996. Extraction of an abundant and unusual protein from soil and comparison with hyhal protein from arbuscular mycorrhizal fungi. Soil Sci. 161:575-585. https://doi.org/10.1097/00010694-199609000-00003
  31. Zeuske, D., and H.C. Weber. 2000. Growth stimulation of Panax ginseng C.A. Meyer (Araliaceae) arising from AMF-isolate inoculation. Symbiosis 29: 213-230.