환경생물 (Korean Journal of Environmental Biology)

  • 제26권4호
  • /
  • Pages.377-384
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  • 2008
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  • 1226-9999(pISSN)
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  • 2287-7851(eISSN)
한국환경생물학회 (Korean Society of Environmental Biology)
권호 표지

환경 스트레스에 의한 세포 내 신호의 이동 경로와 유전적 조절

Genetic Regulation of Cellular Responses and Signal Targeting Pathways Invoked by an Environmental Stress

  • Kim, Il-Sup (Department of Biology, Kyungpook National University) ;
  • Kim, Hyun-Young (Department of Biology, Kyungpook National University) ;
  • Kang, Hong-Gyu (Subtropical Horticulture Research Institute, Cheju National University) ;
  • Yoon, Ho-Sung (Department of Biology, Kyungpook National University)
  • 발행 : 2008.11.30
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초록

A cell is the product of a long period of evolution and can be represented as an optimized system (homeostasis). Stimuli from the outside environment are received by sensory apparatus on the surface of the cell and transferred through complicated pathways and eventually regulate gene expression. These signals affect cell physiology, growth, and development, and the interaction among genes in the signal transduction pathway is a critical part of the regulation. In this study, the interactions of deletion mutants and overexpression of the extracopies of the genes were used to understand their relationships to each other. Also, green fluorescent protein (GFP reporter gene) was fused to the regulatory genes to elucidate their interactions. Cooverexpression of the two genes in extracopy plasmids suggested that patS acts at the downstream of hetR in the regulatory network. The experiments using gfp fusion in different genetic background cells also indicated the epistasis relationships between the two genes. A model describing the regulatory network that controls cell development is presented.

키워드

참고문헌

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