Journal of Integrative Natural Science (통합자연과학논문집)
- Volume 1 Issue 3
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- Pages.230-233
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- 2008
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- 2005-1042(pISSN)
Extension of a 5'- or 3'-end Genomic DNA Sequence by a Single PCR Amplification
- Jeon, Taeck J. (Department of Biology, College of Natural Sciences, Chosun University)
- Received : 2008.10.26
- Accepted : 2008.12.06
- Published : 2008.12.30
Abstract
A simple and rapid method is described for extending the 5'- or 3'-end genomic sequence of a known partial sequence by only a single round of PCR. This method involves digesting and ligating genomic and plasmid DNAs, and amplifying the 5'-upstream or 3'-end downstream sequence of the known DNA sequence, using two primers, one gene specific and the other plasmid specific. A single round of PCR amplification is sufficient to produce gene-specific bands detectable in gels. By using this approach, 5'-end genomic sequence of the D-amoeba sams gene was extended.