Environmental Analysis Health and Toxicology

환경독성보건학회 (The Korean Society of Environmental Health and Toxicology)
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토양선충 Caenorhabditis elegans의 스트레스 관련 유전자 발현을 이용한 시간에 다른 카드뮴의 독성영향

Time-dependent Toxic Effects of Cadmium Chloride on the Stress-related Gene Expression, Growth and Reproduction of the Soil Nematode Caenorhabditis elegans

  • 노지연 (서울시립대학교 환경공학부 독성학연구실) ;
  • 이정경 (서울시립대학교 환경공학부 독성학연구실) ;
  • 권혁두 (서울시립대학교 환경공학부 독성학연구실) ;
  • 최진희 (서울시립대학교 환경공학부 독성학연구실)
  • Roh, Ji-Yeon (Faculty of Environmental Engineering, College of Urban Science, University of Seoul) ;
  • Lee, Jeong-Gyeong (Faculty of Environmental Engineering, College of Urban Science, University of Seoul) ;
  • Kwon, Hyuk-Cu (Faculty of Environmental Engineering, College of Urban Science, University of Seoul) ;
  • Choi, Jin-Hee (Faculty of Environmental Engineering, College of Urban Science, University of Seoul)
  • 발행 : 2008.03.31
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초록

카드뮴은 환경과 인체 위해도에 큰 영향을 미치는 중요한 환경오염물질로 잘 알려져 있다. 본 연구에서는 토양선충인 Caenorhabditis elegans에 카드뮴을 12시간과 48시간으로 나누어 처리하여 시간에 따른 장, 단기적 독성영향을 알아보고자 하였다. 이때 생리학적 수준으로 성장 및 생식을 조사하고, 분자수준에서 스트레스 관련 유전자들의 시간에 따른 발현 정도를 관찰하였다. 생식에서는 단기노출(12시간) 시 그 영향이 대조군에 비해 크게 나타났으며, mtl-2의 스트레스 관련 유전자가 증가하였다 장기 노출(48시간) 시에는 cyp35a2, ape-1, sod-1, ctl-2 유전자가 대조군에 비해 약 $2{\sim}4$배 가량의 발현 증가 결과를 조사할 수 있었다. 본 연구결과들을 통해 스트레스 관련 유전자의 발현을 조사하는 것이 중요하고 민감한 생체지표가 된다는 것과 토양선충 C. elegans는 환경중 오염물질에 대한 장기, 단기적 영향을 평가하기 위한 좋은 생물학적 모델이 된다는 것을 알 수 있었다.

키워드

참고문헌

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