Applied Microscopy

  • 제38권1호
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  • Pages.29-34
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  • 2008
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
한국현미경학회 (Korean Society of Microscopy)
권호 표지

중추신경계통 내 분포하는 zinc 이온의 조직화학적 동정법 비교: I. 광학현미경수준에서

Detection Methods of Histochemically-reactive Zinc in the CNS at the Light Microscopical Level

  • 김이석 (가천의과대학교 의학전문대학원 해부학과) ;
  • 김상현 (가천의과대학교 의학전문대학원 해부학과) ;
  • 이법이 (건국대학교 의학전문대학원 해부학교실) ;
  • 이현숙 (건국대학교 의학전문대학원 해부학교실) ;
  • 김성주 (한림대학교 한림대성심병원 산부인과) ;
  • 조승묵 (가천의과대학교 의학전문대학원 해부학과)
  • Kim, Yi-Suk (Department of Anatomy, Gachon Medical Graduate School) ;
  • Kim, Sang-Hyun (Department of Anatomy, Gachon Medical Graduate School) ;
  • Lee, Beob-Yi (Department of Anatomy, Konkuk Medical Graduate School) ;
  • Lee, Hyun-Sook (Department of Anatomy, Konkuk Medical Graduate School) ;
  • Kim, Sung-Joo (Department of Obstetrics and gynecology, Hallym Sacred Heart Hospital) ;
  • Jo, Seung-Mook (Department of Anatomy, Gachon Medical Graduate School)
  • 발행 : 2008.03.31
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초록

본 연구자는 Zinc Selenium autometallography ($ZnSe^{AMG}$) (Danscher et al., 1997) 염색법을 중심으로 중추신경계통 내 zinc ($Zn^{2+}$)의 분포와 이들을 함유하고 있는 신경종말, 소위 ZEN(zinc-enriched) terminals의 미세구조에 관하여 보고한 바 있다. 이번 연구에서는 다른 몇 가지, 즉 Neo-Timm staining (Danscher, 1982), TSQ fluorescence staining (Frederickson et al.,1987), Zinc transporter-3 Immunohistochemistry ($ZnT3^{IHC}$) (Palmiter et at., 1997) 염색법으로 흰쥐 해마복합체에 분포하는 $Zn^{2+}$를 염색한 후 이들의 염색패턴에서 차이점을 밝히고자 하였다. $ZnSe^{AMG}$ 염색법은 $Zn^{2+}$에 대한 특이성은 다소 떨어지나 광학 및 전자현미경하에서 관찰이 가능하며, 반영구적인 표본으로 보관이 가능하다는 장점이 있었고, TSQ는 $Zn^{2+}$에 대한 특이성이 매우 높을 뿐 아니라 그 염색법이 매우 간단하다는 장점이 있는 반면 형광물질의 안정성과 표본보관이 용이하지 않다는 단점이 있다. 그 외 Neo-Timm 염색법은 TSQ형광염색법과 유사한 염색 패턴을 보였으며, $ZnT3^{IHC}$염색법은 오히려 $ZnSe^{AMG}$에 가까운 염색의 결과를 보였다. 본 연구의 결과는 중추신경계통 내 $Zn^{2+}$에 관한 형태학적 연구에서 기초자료로 활용될 수 있을 것이다.

Small amounts of zinc ions regulate a plentitude of enzymatic proteins, receptors and transcription factors, thus cells need accurate homeostasis of zinc ions. Some neurons have developed mechanisms to accumulate zinc in specific membrane compartment ("vesicular zinc"), which can be evidenced using histochemical techniques. These neurons are the socalled zinc enriched (ZEN) neurons, which accumulate glutamate and zinc inside their synaptic vesicles and release it during synaptic transmission. In the present paper we have studied the distribution of the ZEN terminals in the rat hippo-campus using ZnSe autometallography, Neo-Timm staining, ZnT3 immunohistochemistry and TSQ fluorescence staining.

키워드

참고문헌

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