Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Effects of Electrolyzed Alkaline Reduced Water on Echinostoma hortense Infection and Immune Response in C57BL/6 Mice

C57BL/6 생쥐에서 전해알칼리환원수가 호르텐스극구흡충 감염과 면역에 미치는 영향

  • Kim, Dong-Heui (Department of Environmental Medical Biology and Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Deung, Young-Kun (Department of Environmental Medical Biology and Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Jin, Dan (Department of Immunology and Pathogenic Biology, College of Medicine, Yanbian University) ;
  • Huang, Xue Zhu (Yanbian University Hospital) ;
  • Qi, Xu Feng (Department of Environmental Medical Biology and Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Kim, Kwang-Yong (Department of Environmental Medical Biology and Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Lee, Kyu-Jae (Department of Environmental Medical Biology and Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University)
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실.기초의학연구소) ;
  • 등영건 (연세대학교 원주의과대학 환경의생물학교실.기초의학연구소) ;
  • 김단 (연변대학교 의과대학 면역병리생물학교실) ;
  • 황학수 (연변대학병원) ;
  • 최주봉 (연세대학교 원주의과대학 환경의생물학교실.기초의학연구소) ;
  • 김광용 (연세대학교 원주의과대학 환경의생물학교실.기초의학연구소) ;
  • 이규재 (연세대학교 원주의과대학 환경의생물학교실 기초의학연구소)
  • Published : 2008.03.31
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Abstract

To examine the effect of the electrolyzed alkaline reduced water (ERW) on animal immunity, by employing Echinostoma hortense that is a parasite in the small intestine, the immune response of C57BL/6 was examined. To C57BL/6 mice, Echinostoma hortense metacercaria 15 per animal was in oculate dorsally, the worm was collected after 2 weeks, and the change of goblet cells and mast cells in the mucosa of small intestine was examined, and by using a protein chip, the change of cytokines in the serum was compared and observed. As a result, average 8.3 worms were collected from the C57BL/6 mice infected with E. hortense, and in the group fed with the ERW, average 10 worms were collected. In regard to the examination of the change of goblet cells, in the experimental group infected with E. hortense and fed with the ERW, average 4.3 worms per villus were detected, hence, it was found that the expression of goblet cells was low (p<0.001). Regarding the examination of the change of mast cells, similarly, in the group infected with E. hortense and fed with the ERW, average 11 worms per villus were detected, and it appears to be less than control group (p<0.001). Regarding the expression of cytokines in mouse serum, in comparison of the experiment group infected with E. hortense and control group, in the expression of the Th1 cytokines IL-6, IL-$1{\beta}$, IFN-${\gamma}$, TNF-${\alpha}$, and IL-2, and the Th2 cytokines IL-4, IL-5, IL-10, and IL-13, a significant difference was not detected. In our study, it was found that in the infection of E. hortense, the ERW mediates its effect on the number of goblet cells and mast cells in the intestinal mucosa, and simultaneously, the worm expulsion was delayed, and thus the conclusion that the ERW mediated its effect on the intestinal immunity of mice was obtained.

전해알칼리환원수가 동물의 면역에 미치는 영향을 알아보고자 본 실험을 수행하였다. 소장에서 기생하는 호르텐스극구흡충(Echinostoma hortense)을 이용하여 C57BL/6 생쥐 소장과 혈액에서의 면역반응을 관찰하였다. C57BL/6 생쥐에 호르텐스극구흡충 피낭유충을 마리 당 15개 경구감염 시킨 후 2주 경과하여 충체를 회수하였고 소장 점막의 배상세포와 비만세포의 변화를 관찰하였으며 protein chip을 이용하여 혈청 내 cytokine의 변화를 비교 확인하였다. 그 결과 호르텐스극구흡충을 감염시킨 군에서는 평균 8.3마리, 알칼리환원 수를 급이한 군에서는 평균 10마리의 충체를 회수하였다. 배상 세포 변화의 관찰에서는 호르텐스극구흡충을 감염시키고 전해알칼리환원수를 급이한 실험군이 융모 당 평균 4.3개 관찰되어 배상세포의 발현이 적은 것으로(p<0.001) 나타났다. 비만세포 변화의 관찰에서도 호르텐스극구흡충을 감염시키고 전해알칼리환원수를 급이한 실험군에서 응모 당 평균 11개 관찰되었고 정수물을 급이한 호르텐스 감염 대조군에 비해 적게 나타났다(p<0.001). 마우스 혈청 내에서의 cytokine 발현에서 호르텐스극구흡충에 감염된 실험군과 대조군의 비교에서 Th1 cytokine인 IL-6, IL-$1{\beta}$, IFN-${\gamma}$, TNF-${\alpha}$, Il-2 등과 Th2 cytokine인 IL-4, IL-5, IL-10, IL-13 등의 발현은 모두 유의한 차이가 관찰되지 않았다. 본 연구에서 전기분해 알칼리환원수가 호르텐스극구흡충의 감염에 있어 C57BL/6 마우스에서 장점막조직의 배상세포 수 및 mucin terminal sugar의 변화를 억제하고 아울러 이로 인해 충체배출이 지연되며, 알칼리환원수가 호르텐스극구흡충에 감염된 C57BL/6 마우스에서 혈청 내 cytokine의 변화에는 큰 영향을 미치지 않는다는 결론을 얻었다.

Keywords

References

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