Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Expressing the Tyrosine Phosphatase (CaTPP1) Gene from Capsicum annuum in Tobacco Enhances Cold and Drought Tolerances

  • Hwang, Eul-Won (Department of Biomedical Sciences, Sunmoon University) ;
  • Park, Soo-Chul (Department of molecular physiology and Biochemistry, National Institute of Agricultural Biotechnology) ;
  • Jeong, Mi-Jeong (Department of molecular physiology and Biochemistry, National Institute of Agricultural Biotechnology) ;
  • Byun, Myung-Ok (Department of molecular physiology and Biochemistry, National Institute of Agricultural Biotechnology) ;
  • Kwon, Hawk-Bin (Department of Biomedical Sciences, Sunmoon University)
  • Published : 2008.04.30
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Abstract

As one way to approach to cold defense mechanism in plants, we previously identified the gene for protein-tyrosine phosphatase (CaTPP1) from hot pepper (Capsicum annuum) using cDNA microarray analysis coupled with Northern blot analysis. We showed that the CaTPP1 gene was strongly induced by cold, drought, salt and ABA stresses. The CaTPP1 gene was engineered under control of CaMV 35S promoter for constitutive expression in transgenic tobacco plants by Agrobacterium-mediated transformation. The resulting CaTPP1 transgenic tobacco plants showed significantly increased cold stress resistance. It also appeared that some of the transgenic tobacco plants showed increased drought tolerance. The CaTPP1 transgenic plants showed no visible phenotypic alteration compared to wild type plants. These results showed the involvement of protein tyrosine phosphatase in tolerance of abiotic stresses including cold and drought stress.

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References

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