Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Effect of an Aqueous Chlorine Dioxide Generator and Effect on Disinfection of Fresh Fruits and Vegetables by Immersion Washing

이산화염소수 생성기의 생성효율 및 과.채류에 대한 침지 세정 살균효과

  • Published : 2008.04.28
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Abstract

We investigated the optimum concentration of a $NaClO_2$ solution and the amount of gaseous $Cl_2$ for production of high yield and purity of aqueous $ClO_2$ by use of a gaseous chlorine-chlorite $ClO_2$ generator. This system produced lower concentrations of chlorine dioxide and is applicable for direct-use in food processing as a cleaner and sanitizer. The concentration of $NaClO_2$ solution and the amount of gaseous $Cl_2$ was varied from 0.01-0.1% and 100-1,000 g/hr, respectively. The concentrations of chlorite, chlorate, FAC (free available chlorine), and chlorine dioxide that were produced increased with increasing concentration of $NaClO_2$ solution and with the amount of gaseous $Cl_2$. The optimum concentration of $NaClO_2$ solution and amount of gaseous $Cl_2$ were 0.1% and 900 g/hr respectively. $ClO_2$ and FAC produced at these concentrations were 882.0 ppm and 8.0 ppm, with no detection of chlorite and chlorate. The yield and purity of $ClO_2$ were 97.0% and 96.0% respectively. Immersion-cleaning experiments showed that this protocol decreased the level of CFU/g by $10^3$- to $10^4$-fold, with a similar effect on fruit.

이산화염소수 발생 최적조건을 검토하기 위하여 생성원료인 $NaClO_2$ 농도(0.01-0.1%)와 $Cl_2$ gas 투입량(100-1,000 g/hr)에 따른 chlorite, chlorate, FAC 및 chlorine dioxide의 생성량을 조사하였다. Chlorate, FAC 및 chloride dioxide 생성량은 $NaClO_2$ 및 $Cl_2$ 투입량이 증가할 수록 생성량이 증가하는 경향을 나타내었다. 이산화염소수 최적 생성조건은 0.1% $NaClO_2$, 900 g/hr $Cl_2$ gas 농도였으며 발생한 이산화염소수의 $ClO_2$는 882 ppm, FAC는 8.0 ppm, chlorite는 검출되지 않았고 chlorate는 43.32 ppm이었다. 이때의 수율과 순도는 각각 97.0%, 96.0%였다. 상추, 치콘, 깻잎, 케일의 채소류에 대한 세정 침지 살균에서는 이산화염소수 농도의존성 감균 효과를 나타내어 3-4 log의 총균수 감소 효과가 있었다. 과일의 경우에도 농도 의존성 감균 효과는 동일하였으며 대략 3-5 log의 총균수 감균 효과가 있었다.

Keywords

References

  1. Lee, S.Y., Gray, P.M., Dougherty, R.H. and Kang, D.H. (2004) The use of chlorine dioxide to control Alicyclobacillus acidoterrestric spores in aqueous suspension and on apples. Food Microbiol., 92, 121-127 https://doi.org/10.1016/j.ijfoodmicro.2003.09.003
  2. Cherry, J.P. (1999) Improving the safety of fresh produce with antimicrobials. Food Technol., 53, 54-59
  3. Benarde, M.A., Israel, B.M., Oliveri, V.P. and Granstrom, M.L. (1965) Efficiency of chlorine dioxide as a bactericide. Appl. Microbiol., 13, 776-780
  4. Lillard, H.S. (1979) Levels of chlorine dioxide of equivalent bactericidal effect in poultry processing water. J. Food Sci., 44, 1594-1597 https://doi.org/10.1111/j.1365-2621.1979.tb09097.x
  5. Winniczuk P.P. and Parish, M.E. (1997) Minimum inhibitory concentration of antimicrobials against microorganisms related to citrus juice. Food Microbiol., 14, 373-381 https://doi.org/10.1006/fmic.1997.0103
  6. Chang, C.Y., Hsieh, Y.H., Shin, I.C., Hsu, S.S. and Wang, K.H. (2000) The formation and control of disinfection by-products using chlorine dioxide. Chemosphere, 41, 1181-1186 https://doi.org/10.1016/S0045-6535(00)00010-2
  7. Junli, H., Li, W., Nanqi, R., Fang, M. and Juni, L. (1997) Disinfection effect of chlorine dioxide on bacteria in water. Water Res., 31, 607-613 https://doi.org/10.1016/S0043-1354(96)00275-8
  8. Food and Drug Administration. (2007) Guidance for industry-guide to minimize microbial food safety hazards for fresh fruits and vegetables. Available from: http://www.cfsan.fda.gov. Accessed Fed. 27
  9. Park, H.K., Park, B.K., Shin, H.W., Park, D.W., Kim, Y.S., Cho, Y.H., Lee, K.H., Kang, K.J., Jeon, D.H., Park, K.H. and Ha, S.D. (2005) Evaluation of effectiveness of sanitizers and disinfectants used in domestic food processing plant. Korean J. Food Sci. Technol., 37, 1042-1047
  10. United States Environmental Protection Agency. (2007) Alternative disinfectants and oxidants guidance manual. Available from: http://www.epa.gov. Accessed Fed. 27
  11. Shin, H.S. and Oh, Y.S. (1999) Analysis of the contents in stabilized chlorine dioxide. Anal. Sci. Technol., 12, 403-407
  12. Kim, J.M. (2001) Use of chlorine dioxide as a biocide in the food industry. Food Industry and Nutrition, 6, 33-39
  13. APHA, AWWA and WEF. (2005) Standard methods for the examination of water and wastewater, 21th Edition. American Public Health Association. Washington DC, U.S.A.
  14. Radziminski, C., Ballantyne, L., Hodson, J., Creason, R., Andrews, R.C. and Chauret, C. (2002) Disinfection of Bacillus subtilis spores with chlorine dioxide: a bench-scale and pilot-scale study. Water Res., 36, 1629-1639 https://doi.org/10.1016/S0043-1354(01)00355-4
  15. Gordon, G., Slootmaekers, B., Tachiyashiki, S. and Wood, D.W. (1990) Minimizing chlorite ion and chlorate ion in water treated with chlorine dioxide. J. AWWA, 82, 160-165 https://doi.org/10.1002/j.1551-8833.1990.tb06947.x
  16. Latshaw, C.L. (1994) Chlorine dioxide: effective, board-spectrum biocide for white-water systems. Tappi J., 78, 163-166
  17. Veschetti, E., Cittadini, B., Maresca, G., Citti, G. and Ottaviani, M. (2005) Inorganic by-products in waters disinfected with chlorine dioxide. Microchem. J., 79, 165-170 https://doi.org/10.1016/j.microc.2004.10.017
  18. Lillard, H.S. (1979) Levels of chlorine and chlorine dioxide of equivalent bactericidal effect in poultry processing water. J. Food Sci., 44, 1594-1597 https://doi.org/10.1111/j.1365-2621.1979.tb09097.x
  19. Han, Y., Guentert, A.M., Smith, R.S., Linton, R.H. and Nelson, P.E. (1999) Efficacy of chlorine dioxide gas as a sanitizer for tanks used for aseptic juice storage. Food Microbiol., 16, 53-61 https://doi.org/10.1006/fmic.1998.0211
  20. Singh, N., Singh, R.K., Bhunia, A.K. and Stroshine, R.L. (2002) Efficacy of chlorine dioxide, ozone, and thyme essential oil or a sequental washing in killing Eschrichia coli O157:H7 on lettuce and baby carrots. WLT., 35, 720-729