Isolation and Identification of Phosphate Solubilizing Bacteria from Chinese Cabbage and Their Effect on Growth and Phosphorus Utilization of Plants

  • Poonguzhali, Selvaraj (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Madhaiyan, Munusamy (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
  • Published : 2008.04.30

Abstract

Phosphate solubilizing bacteria (PSB) were isolated from the rhizosphere of Chinese cabbage and screened on the basis of their solubilization of inorganic tricalcium phosphate in liquid cultures. Ten strains that had higher solubilization potential were selected, and they also produced indole-3-acetic acid, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and siderophores. The strains were identified to be members of Pseudomonas, by 16S rDNA sequence analysis. Seed bacterization with PSB strains increased the root elongation and biomass of Chinese cabbage in seedling culture, although they had no effect on phosphorus uptake of plants. The plant growth promotion by PSB in this study could be due to the production of phytohormones or mechanisms other than phosphate solubilization, since they had no effect on P nutrition.

Keywords

References

  1. Behrendt, U., A. Ulrich, and P. Schumann. 2003. Fluorescent pseudomonads associated with the phyllosphere of grasses; Pseudomonas trivialis sp. nov., Pseudomonas poae sp. nov. and Pseudomonas congelans sp. nov. Int. J. Syst. Evol. Microbiol. 53: 1461-1469 https://doi.org/10.1099/ijs.0.02567-0
  2. Glick, B. R., D. M. Penrose, and J. Li. 1998. A model for the lowering of plant ethylene concentrations by plant growthpromoting bacteria. J. Theor. Biol. 190: 63-68 https://doi.org/10.1006/jtbi.1997.0532
  3. Gyaneshwar, P., K. G. Naresh, L. J. Parekh, and P. S. Poole. 2002. Role of soil micro-organisms in improving P nutrition of plants. Plant Soil 245: 83-93 https://doi.org/10.1023/A:1020663916259
  4. Higgins, D. G., A. J. Bleasby, and R. Fuchs. 1992. CLUSTAL V: Improved software for multiple sequence alignment. Comput. Appl. Biosci. 8: 189-191
  5. Izumi, H., I. C. Anderson, I. J. Alexander, K. Killham, and E. R. B. Moore. 2006. Endobacteria in some ectomycorrhiza of Scotspine (Pinus sylvestris). FEMS Microbiol. Ecol. 56: 34-43 https://doi.org/10.1111/j.1574-6941.2005.00048.x
  6. Jackson, M. L. 1973. Soil Chemical Analysis. Prentice Hall of India Private Ltd., New Delhi, India
  7. Jafra, S., and J. M. vander Wolf. 2004. Fast screening method for detection of acyl-HSL-degrading soil isolates. J. Microbiol. Methods 57: 415-420 https://doi.org/10.1016/j.mimet.2004.01.015
  8. Kang, S. H., H. S. Cho, H. Cheong, C. M. Ryu, J. F. Kim, and S. H. Park. 2007. Two bacterial entophytes eliciting both plant growth promotion and plant defense on pepper (Capsicum annuum L.). J. Microbiol. Biotechnol. 17: 96-103
  9. Karamanoli, K. and S. E. Lindow. 2006. Disruption of N-acyl homoserine lactone-mediated cell signaling and iron acquisition in epiphytic bacteria by leaf surface compounds. Appl. Environ. Microbiol. 72: 7678-7686 https://doi.org/10.1128/AEM.01260-06
  10. Kucey, R. M. N. 1983. Phosphate solubilizing bacteria and fungi in various cultivated and virgin Alberta soils. Can. J. Soil Sci. 63: 671-678 https://doi.org/10.4141/cjss83-068
  11. Laheurte, F. and J. Berthelin. 1988. Effect of a phosphatesolubilizing bacteria on maize growth and root exudation over four levels of labile phosphorus. Plant Soil 105: 11-17 https://doi.org/10.1007/BF02371137
  12. Murphy, J. and J. P. Riley. 1962. A modified single solution method for the determination of phosphate in natural waters. Anal. Chim. Acta 27: 31-36 https://doi.org/10.1016/S0003-2670(00)88444-5
  13. Nautiyal, C. S. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol. Lett. 170: 265-270 https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
  14. Pikovskaya, R. I. 1948. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiologiya 17: 362-370
  15. Poonguzhali, S., M. Madhaiyan, M. Thangaraju, J. H. Ryu, K. Y. Chung, and T. M. Sa. 2005. Effects of co-cultures, containing N-fixer and P-solubilizer, on the growth and yield of Pearl millet (Pennisetum glaucum (L.) R. Br.) and Blackgram (Vigna mungo L.). J. Microbiol. Biotechnol. 15: 903-908
  16. Poonguzhali, S., M. Madhaiyan, and T. M. Sa. 2006. Cultivationdependent characterization of rhizobacterial communities from field grown Chinese cabbage Brassica campestris ssp. pekinensis and screening of traits for potential plant growth promotion. Plant Soil 286: 167-180 https://doi.org/10.1007/s11104-006-9035-1
  17. Poonguzhali, S., M. Madhaiyan, and T. M. Sa. 2007. Production of acyl-homoserine lactone quorum-sensing signals is widespread in Gram-negative Methylobacterium. J. Microbiol. Biotechnol. 17: 226-233
  18. Poonguzhali, S., M. Madhaiyan, and T. M. Sa. 2007. Quorumsensing signals produced by plant-growth promoting Burkholderia strains under in vitro and in planta conditions. Res. Microbiol. 158: 287-294 https://doi.org/10.1016/j.resmic.2006.11.013
  19. Richardson, A. E., P. A. Hadobas, and J. E. Hayes. 2000. Acid phosphomonoesterase and phytase activities of wheat (Triticum aestivum L.) roots and utilization of organic phosphorus substrates by seedlings grown in sterile culture. Plant Cell Environ. 23: 397-405 https://doi.org/10.1046/j.1365-3040.2000.00557.x
  20. Ryu, J. H., M. Madhaiyan, S. Poonguzhali, W. J. Yim, P. Indiragandhi, K. A. Kim, R. Anandham, J. C. Yun, and T. M. Sa. 2006. Plant growth substances produced by Methylobacterium spp. and their effect on the growth of tomato (Lycopersicon esculentum L.) and red pepper (Capsicum annuum L.). J. Microbiol. Biotechnol. 16: 1622-1628
  21. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, New York, U.S.A.
  22. Shin, W. S., J. H. Ryu, S. W. Choi, C. W. Kim, R. S. Gadagi, M. Madhaiyan, S. Seshadri, J. B. Chung, and T. M. Sa. 2005. Solubilization of hardly soluble phosphates and growth promotion of maize (Zea mays L.) by Penicillium oxalicum isolated from rhizosphere. J. Microbiol. Biotechnol. 15: 1273-1279
  23. Sundara, B., V. Natarajan, and K. Hari. 2002. Influence of phosphorus solubilizing bacteria on the changes in soil available phosphorus and sugarcane and sugar yields. Field Crops Res. 77: 43-49 https://doi.org/10.1016/S0378-4290(02)00048-5
  24. Tabatabai, M. A. and J. M. Bremner. 1969. Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol. Biochem. 1: 301-307 https://doi.org/10.1016/0038-0717(69)90012-1