Maxillofacial Plastic and Reconstructive Surgery

  • 제30권4호
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  • Pages.323-332
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  • 2008
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  • 2288-8101(pISSN)
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  • 2288-8586(eISSN)
대한악안면성형재건외과학회 (Korean Association of Maxillofacial Plastic and Reconstructive Surgeons)
권호 표지

PLLA/HA Composite Scaffold와 골수 줄기세포를 이용한 조직공학적 골재생에 대한 연구

BONE TISSUE ENGINEERING USING PLLA/HA COMPOSITE SCAFFOLD AND BONE MARROW MESENCHYMAL STEM CELL

  • 김병렬 (고려대학교 의과대학 치과, 구강악안면외과학교실) ;
  • 장현석 (고려대학교 의과대학 치과, 구강악안면외과학교실) ;
  • 임재석 (고려대학교 의과대학 치과, 구강악안면외과학교실) ;
  • 이의석 (고려대학교 의과대학 치과, 구강악안면외과학교실) ;
  • 김동현 (고려대학교 의과대학 치과, 구강악안면외과학교실)
  • Kim, Byeong-Yol (Department of Oral & Maxillofacial Surgery, School of Medicine, Korea University) ;
  • Jang, Hyon-Seok (Department of Oral & Maxillofacial Surgery, School of Medicine, Korea University) ;
  • Rim, Jae-Suk (Department of Oral & Maxillofacial Surgery, School of Medicine, Korea University) ;
  • Lee, Eui-Seok (Department of Oral & Maxillofacial Surgery, School of Medicine, Korea University) ;
  • Kim, Dong-Hyun (Department of Oral & Maxillofacial Surgery, School of Medicine, Korea University)
  • 발행 : 2008.07.31
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초록

Aim of the study: Scaffolds are crucial to tissue engineering/regeneration. Biodegradable polymer/ceramic composite scaffolds can overcome the limitations of conventional ceramic bone substitutes such as brittleness and difficulty in shaping. In this study, poly(L-lactide)/hydroxyapatite(PLLA/HA) composite scaffolds were fabricated for in vivo bone tissue engineering. Material & methods: In this study, PLLA/HA composite microspheres were prepared by double emulsion-solvent evaporation method, and were evaluated in vivo bone tissue engineering. Bone marrow mesenchymal stem cell from rat iliac crest was differentiated to osteoblast by adding osteogenic medium, and was mixed with PLLA/HA composite scaffold in fibrin gel and was injected immediately into rat cranial bone critical size defect(CSD:8mm in diameter). At 1. 2, 4, 8 weeks after implantation, histological analysis by H-E staining, histomorphometric analysis and radiolographic analysis were done. Results: BMP-2 loaded PLLA/HA composite scaffolds in fibrin gel delivered with osteoblasts differentiated from bone marrow mesenchymal stem cells showed rapid and much more bone regeneration in rat cranial bone defects than control group. Conclusion: This results suggest the feasibility and usefulness of this type of scaffold in bone tissue engineering.

키워드

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