Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Inhibitory Activities of Red Ginseng Acidic Polysaccharide in Platelet Aggregation

  • Lee, Whi-Min (College of Veterinary Medicine, Kyungpook National University) ;
  • Kamruzzaman, S.M. (College of Veterinary Medicine, Kyungpook National University) ;
  • Song, Yong-Bum (KT & G Central Research Institute) ;
  • Cho, Jae-Youl (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Park, Hwa-Jin (College of Biomedical Science and Engineering, Inje University) ;
  • Rhee, Man-Hee (College of Veterinary Medicine, Kyungpook National University)
  • Published : 2008.03.31
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Abstract

Red ginseng acidic polysaccharide (RGAP), isolated from Korean red ginseng (Panax ginseng C.A. Meyer), has been shown to have a variety of biological functions such as immunostimulating and anti-tumor activities. In the present study, we investigated whether RGAP inhibited ligand-induced platelet aggregation. The washed platelet-rich plasma was prepared from male SD rats with successive centrifugation. The platelets $(10^8/ml)$ were preincubated with 1 mM of $CaCl_2$ for 2 min either in the presence or in the absence of RGAP $(10{\sim}50\;{\mu}g/ml)$ and were stimulated with collagen (2.5 ${\mu}g/ml$) and thrombin (0.1 U/ml). RGAP dose-dependently inhibited thrombin-induced platelet aggregation with $IC_{50}$ value of $26.2{\pm}2.0$ ${\mu}g/ml$. In collagen-induced platelet aggregation, RGAP inhibited the reaction with an $IC_{50}$ value of $31.5{\pm}3.0\;{\mu}g/ml$. RGAP potently suppressed the intracellular calcium ion, which was stimulated by thrombin (0.1 U/ ml). Among mitogen-activated protein kinase (MAPK) subtypes, the extracellular signal-regulated kinase (ERK) 1/2 and p38 MAPK were analyzed in the present study. RGAP inhibited the phosphorylation of ERK2 and p38 MAPK, which was activated by collagen (2.5 ${\mu}g/ml$). Finally, these results suggested that besides saponin fraction, RGAP take an important role in the preventive effect of Korean red ginseng against cardiovascular disease such as thrombosis and atherosclerosis.

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References

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