The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Inhibitory Effect of Soyosangagamhwajae on Melanin Synthesis and its Action Mechanism in B16F10 Mouse Melanoma Cell

소요산가감화제(逍遙散加減化製)의 멜라닌 생성 억제와 작용기전에 관한 연구

  • Kim, Eun-Seop (Dept. of Oriental Medicine Graduate School, Daejeon University) ;
  • Lim, Hyun-Jung (Dept. of Oriental Medicine Graduate School, Daejeon University) ;
  • Shin, Sun-Mi (Dept. of Oriental Medicine Graduate School, Daejeon University) ;
  • Kim, Soo-Min (Dept. of Oriental Medicine Graduate School, Daejeon University) ;
  • Lee, Jung-Eun (Dept. of Oriental Medicine Graduate School, Daejeon University) ;
  • Yoo, Dong-Youl (Dept. of Oriental Medicine Graduate School, Daejeon University)
  • 김은섭 (대전대학교 한의과대학 부인과교실) ;
  • 임현정 (대전대학교 한의과대학 부인과교실) ;
  • 신선미 (대전대학교 한의과대학 부인과교실) ;
  • 김수민 (대전대학교 한의과대학 부인과교실) ;
  • 이정은 (대전대학교 한의과대학 부인과교실) ;
  • 유동열 (대전대학교 한의과대학 부인과교실)
  • Published : 2008.02.29
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Abstract

Purpose: This study was performed to determine the inhibitory effect of Soyosangagamhwajae(SYG) on melanin synthesis in B16F10 mouse melanoma cell. Methods: The Inhibitory effects of Soyosangagamhwajae(SYG) on melanin synthesis were determined by in-vitro assay. To elucidate inhibitory effects of SYG on melanin synthesis, we determined the melanin release in B16F10 cell. And to investigate the action mechanism, we assessed the gene expression of tyrosinase, TRP-1, TRP-2. PKA, $PKC{\beta}$ in B16F10 cell. Results: 1. SYG significantly inhibited melanin-release in B16F10 cell. 2. SYG significantly inhibited mushroom tyrosinase activity in vitro. 3. SYG significantly suppressed the expression of tyrosinase in B16F10 cell. 4. SYG significantly suppressed the expression of TRP-1, TRP-2 in B16F10 cell. 5. SYG significantly suppressed the expression of PKA, $PKC{\beta}$ in B16F10 cell. Conclusion: From these results, it may be concluded that SYG has the antimelanogenetic effect.

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