Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Study on the Vasorelaxant Mechanism of the Butanol Extract of Euonymus alatus

귀전우(鬼箭羽) 부탄올 추출물의 혈관이완 기전에 대한 연구

  • Li, Xiang (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kang, Dae-Gill (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Jun-Kyoung (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim, Seung-Ju (Korea Institute of Oriental Medicine) ;
  • Choi, Deok-Ho (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Kye-Bok (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Cui, Hao-Zhen (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Yeom, Ki-Bok (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Ho-Sub (Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 리향 (원광대학교 한의학전문대학원) ;
  • 강대길 (원광대학교 한의학전문대학원) ;
  • 이준경 (원광대학교 한의학전문대학원) ;
  • 김승주 (한국 한의학 연구원) ;
  • 최덕호 (원광대학교 한의학전문대학원) ;
  • 이계복 (원광대학교 한의학전문대학원) ;
  • 최호진 (원광대학교 한의학전문대학원) ;
  • 염기복 (원광대학교 한의학전문대학원) ;
  • 이호섭 (원광대학교 한의학전문대학원)
  • Published : 2008.02.25
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Abstract

The butanol extract of Euonymus alatus (BEA) induced dose-dependent relaxation of phenylephrine-precontracted aorta, which was abolished by removal of functional endothelium. Pre-treatment of the endothelium-intact aortic tissues with $N^G-nitro-L-arginine methylester$ (L-NAME), and 1 H-[1,2,4]-oxadiazole- [$4,3-{\alpha}$]-quinoxalin-1-one (ODQ) inhibited the relaxation induced by BEA, respectively. BEA-induced vascular relaxation was not blocked by glibenclamide, tetraethylammonium (TEA), indomethacin, atropine, propranolol, verapamil, and diltiazem, respectively. Moreover, BEA inhibits phenylephrine-induced vascular constriction in a dose-dependent manner. These results suggest that BEA relaxes vascular smooth muscle via endothelium-dependent nitric oxide/cGMP signaling.

Keywords

References

  1. Bosnjak, Z.J. Ion channels in vascular smooth muscle: Anesthesiology. 79: 11392-11401, 1993
  2. Rees, D.D., Palmer, R.M., Schulz, R., Hodson, H.F., Moncada, S. Characterization of three inhibitors endotherial Nitric oxide synthase in vitro and in vivo: Br. J. Pharmacol. 101: 746-752, 1990 https://doi.org/10.1111/j.1476-5381.1990.tb14151.x
  3. Vane, J.R., Anggard, E.E., Botting, R.M. Regulatory function of the vascular endothelium: New Med. 323(1):27-36, 1990 https://doi.org/10.1056/NEJM199007053230106
  4. Luscher, T.F. The endothelium as a target and mediator of cardiovascular disease: Eur. J. Clin. Invest. 23: 670-685, 1993 https://doi.org/10.1111/j.1365-2362.1993.tb01285.x
  5. Moncada, S., Palmer, R.M., Higgs, E.A. Nitric oxide : Physiology, pathophysiology and pharmacology: Phamacol Rev. 43: 109-142, 1992
  6. Forstermann, U., Closs, E.I., Pollock, J.S., Nakane, M., Schwarz, P., Gath, I., Kleinert, H. Nitric oxide synthase isozyme-characterization, purification, molecular 4 cloning and function: Hypertension 23: 1121-1131, 1994 https://doi.org/10.1161/01.HYP.23.6.1121
  7. Furchgott, R.F., Zawadzki, J.V. The obligatory role of endothelial cells in therelaxation of arteterial smooth muscle by acetylcholine: Nature 288: 373-376, 1980 https://doi.org/10.1038/288373a0
  8. Peach, M.J., Loeb, A.L., Singer, H.A., Saye, J. Endothelium- derived vascular relaxation factor: Hypertension 7: 94-100, 1985 https://doi.org/10.1161/01.HYP.7.3_Pt_2.I94
  9. Sakuma, I.D., Stuehr, D.J., Gross, S.S., Nathan, C., Levi, R. Identification of arginine as a precursor of endothelium-derived nitric oxide: Proc. Natl. Acad. Sci. 85: 8864-8867, 1989
  10. Kuramochi, T., Chu, J., Suga, T. Gou-Teng (From Uncaria rhynchophylla Miquel)-induced endothelium-dependent and independent relaxations in the isolated rat aorta: Life Sci. 54: 2061-2069, 1994 https://doi.org/10.1016/0024-3205(94)00715-2
  11. Goto, H., Shimada, Y., Tanigawa, K., Sekiya, N., Shitani, T., Terasawa, K. Effect of Uncariae ramulus et Uncus on endothelium in spontaneously hypertensive rats: Am. J. Chin. Med. 27: 339-345, 1999 https://doi.org/10.1142/S0192415X99000380
  12. Goto, H., Sakakibara, I., Shimada, Y., Kasahara, Y., Terasawa, K. Vasodilator effect of extract prepared from Uncariae ramulus on isolated rat aorta: Am. J. Chin. Med. 28: 197-203, 2000 https://doi.org/10.1142/S0192415X00000246
  13. Kamata, K., Noguchi, M., Nagai, M. Hypotensive effects of thospermic acid B isolated from the extract of Salviae miltiorrhizae radix in the rat: Gen. Phamacol. 25: 69-73, 1994
  14. Chiou, W.F., Chang, P.C., Chou, C.J., Chen, C.F. Protein constituent contributes to the hypotensive and vasorelaxatant activities of Cordyceps sinensis: Life Sci. 66: 1369-1376, 2000 https://doi.org/10.1016/S0024-3205(00)00445-8
  15. Kang, D.G., Sohn, E.J., Kwon, E.K., Han, J.H., Oh, H.C., Lee, H.S. Effects of berberine on angiotensin-converting enzyme and NO/cGMP system in vessels: Vascul. Pharmacol. 39: 281-286, 2002 https://doi.org/10.1016/S1537-1891(03)00005-3
  16. 신민교 외. 中藥大辭典. 鼎談 2권, pp 514-516, 1997
  17. Rubanyi, G.M. Endothelium-derived relaxing and contracting factors: J. Cell. Biochem. 46: 27-36, 1991 https://doi.org/10.1002/jcb.240460106
  18. MacNaul, K.L., Hutchinson, N.I. Differential of iNOS and cNOS mRNA in human vascular smooth muscle cells and endothelial cells under normal and inflammatory conditions: Biochem. Biophys. Res. Commun. 196: 1330-1334, 1993 https://doi.org/10.1006/bbrc.1993.2398
  19. Meng, W., Ma, J., Ayata, C., Hara, H., Huang, P.L., Fishman, M.C., Moskowitz, M.A. Ach dilates pial arterioles in endothelial and neuronal NOS knockout mice by NO-dependent mechanisms: Am. J. Physiol. 271: H1145-H1150, 1996
  20. Arnold, W.P., Mittal, C.K., Katsuki, S., Murad F. Nitric oxide activates guanylate cyclase and increase guanosine 3':5'-cyclic monophosphate levels in various tissue preparations: Proc. Natl. Acad. Sci. 74: 3203-3207, 1977
  21. Jaiswal, N.L., Lambrecht, G., Mutschler, E., Tache, R., Malik, K.U. Pharmacological characterization of the vascular muscarinic receptors mediating relaxation and contraction in rabbit aorta: J. Pharmacol. Exp. Ther. 258: 842-850, 1991
  22. Hutcheson, I.R., Griffith, T.M. Central role of intracellular calcium stores in acute flow-and agonist-evoked endothelial nitric oxide release: Br. J. Pharmacol. 122: 117-125, 1997 https://doi.org/10.1038/sj.bjp.0701340
  23. Sharma, N.R., Davis, M.J. Substance P-induced calcium entry in endothelial cells is secondary to depletion of intracelluar stores: Am. J. Physiol. 268: H962-H973, 1995
  24. Guerrero, M.F., Puebla, P., Carron, R., Martin, M.L., San, R.L. Quercetin 3,7-dimethyl ether: a vasorelaxant flavonoid isolated from Croton schiedeanus Schlecht: J. Pharm. Pharmacol. 54: 1373-1378, 2002 https://doi.org/10.1211/002235702760345455
  25. Lemos, V.S., Freitas, M.R., Muller, B., Lino, Y.D., Queiroga, C.E., Cortes, S.F. Dioclein, a new nitric oxide- and endothelium-dependent vasodilator flavonoid: Eur. J. Pharmacol. 386: 41-46, 1999 https://doi.org/10.1016/S0014-2999(99)00747-5
  26. Ko, F.N., Huang, T.F., Teng, C.M. Vasodilatory action mechanisms of apigenin isolated from Apium graveolens in rat thoracic aorta: Biochim. Biophys. Acta. 1115: 69-74, 1991 https://doi.org/10.1016/0304-4165(91)90013-7
  27. Van, B.C, Cauvin, C., Johns, A., Leijten, P., Yamamoto, H. $Ca^{2+}$ regulation of vascular smooth muscle: Fed. Proc. 45: 2746-2751, 1986
  28. Nevala, R., Paukku, K., Korpela, R., Vapaatalo, H. Calcium-sensitive potassium channel inhibitors antagonize genistein- and daidzein-induced arterial relaxation in vitro: Life Sci. 69: 1407-1417, 2001 https://doi.org/10.1016/S0024-3205(01)01233-4
  29. Mikkelsen, E.O., Thastrup, O., Christensen, S.B. Effects of thapsigargin in isolated rat thoracic aorta: Pharmacol. Toxicol. 62: 7-11, 1988 https://doi.org/10.1111/j.1600-0773.1988.tb01835.x
  30. Delaflotte, S., Auguet, M., DeFeudis, F.V., Baranes, J., Clostre, F., Drieu, K., Braquet, P. Endothelium-dependent relaxations of rabbit isolated aorta produced by carbachol and by Ginkgo biloba extract: Biomed. Biochim. Acta 43: S212-216, 1984
  31. Busse, R., Fleming, I. Endothelial dysfunction in atherosclerosis: J. Vasc. Res. 33(3):181-194, 1996 https://doi.org/10.1159/000159147
  32. Tanner, M.A., Bu, X., Steimle, J.A., Myers, P.R. The direct release of nitric oxide by gypenosides derived from the herb Gynostemma pentaphyllum: Nitric Oxide 3(5):359-365, 1999 https://doi.org/10.1006/niox.1999.0245