Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Effect of Harvest Date on Antioxidant of Dendranthema zawadskii var. latilobum (Maxim.) Kitam and D. zawadskii var. yezoense (Maek.) Y.M. Lee & H.J. Choi

구절초와 남구절초의 항산화 효과에 미치는 수확시기의 영향

  • Published : 2008.04.30
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Abstract

This study was conducted to compare the antioxidative effects of Dendranthema zawadskii var. latilobum (Maxim.) Kitam and D. zawadskii var. yezoense (Maek.) Y.M. Lee & H.J. Choi due to the harvest date. Harvested samples at May 9th (early), July 17th (middle) and September 3rd (late stage) were extracted with 80% ethanol, and biological activities and antioxidant substance contents were detected. The earlier harvested samples showed the higher moisture contents and extraction yields. DPPH radical scavenging effect of early harvested D. zawadskii var. latilobum (Maxim.) Kitam ($RC_{50}$ = $0.128\;mg\;{\cdot}\;mL^{-1}$) was similar to BHT ($0.121\;mg\;{\cdot}\;mL^{-1}$). ABTS radical scavenging effects of both species harvested at early stage were higher than that of ascorbic acid which was well-known natural antioxidant. However, both species harvested at late stage showed the highest $Fe^{2+}$ chelating effect, but it was lower than that of EDTA. Total polyphenol and flavonoid contents of both species were higher when samples were harvested earlier stage. However, both species harvested at early stage contained more total polyphenols (79.93 and $75.10\;mg\;{\cdot}\;g^{-1}$) than flavonoids (57.84 and $54.91\;mg\;{\cdot}\;g^{-1}$).

구절초와 남구절초의 수확 시기에 따른 항산화 효과를 비교하기 위하여 본 연구를 실시하였다. 구절초와 남구절초를 생육초기(5월 9일), 중기(7월 17일) 그리고 생육 후기(9월 3일)에 각각 수확한 후 80% 에탄올로 추출하여 radical 소거활성 및 항산화 물질 함량을 측정하였다. 수확 시기가 늦어질수록 식물체의 수분 함량과 추출 수율이 낮아졌다. DPPH 및 ABTS radical 소거능은 이른 시기에 수확한 시료일수록 활성이 높은 경향을 보였다. 구절초의 DPPH radical 소거능은 생육 초기에 수확한 시료가 $0.128mg\;{\cdot}\;mL^{-1}$의 $RC_{50}$값을 나타내 $BHT(RC_{50}=0.121mg\;{\cdot}\;mL^{-1})$와 비슷한 소거능을 보였고, 생육 초기에 수확한 남구절초와 구절초는 천연 항산화제인 ascorbic acid보다도 높은 ABTS radical 소거활성을 나타냈다. 그러나 $Fe^{2+}$ chelating 효과의 경우, 구절초를 생육 후기에 수확하는 것이 초기나 중기에 수확하는 것 보다 높았으며, EDTA에 비해서도 훨씬 낮았다. 두 종 모두 수확 시기가 빠를수록 총 폴리페놀 및 플라보노이드의 함량이 높은 경향을 보였으며, 생육 초기에 수확한 구절초와 남구절초의 총 폴리페놀 함량은 각각 79.93 및 $75.10mg\;{\cdot}\;g^{-1}$로 총 플라보노이드 함량(57.84, $54.91mg\;{\cdot}\;g^{-1}$)에 비해 높았다.

Keywords

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