Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Plant Regeneration via Multiple Shoot Formation from Sucker Explant of Hybrid Blackberry (Rubus fruticosus L. ${\times}$ R. parvifolius L.)

교잡종 블랙베리(Rubus fruticosus L. ${\times}$ R. parvifolius L.)의 근맹아 절편체로부터 다경유도를 통한 식물체 재분화

  • 신정순 ((주)파낙시아 자원식물연구소) ;
  • 이종천 ((주)파낙시아 자원식물연구소) ;
  • 심옥경 ((주)파낙시아 자원식물연구소) ;
  • 윤태영 ((주)파낙시아 자원식물연구소) ;
  • 조한직 ((주)파낙시아 자원식물연구소) ;
  • 김이엽 ((주)파낙시아 자원식물연구소)
  • Published : 2008.04.30
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Abstract

This study was carried out to induce plant regeneration via multiple shoot formation from sucker explants of Rubus fruticosus L. ${\times}$ R. parvifolius L. To induce adventitious shoots, the sucker explants were sterilized in 1.2% sodium hypochlorite (NaOCl) solution, and then were cultured on the full and 1/2 MS solid medium supplemented with BA (0.1, 0.5, 1.0, $2.0mg{\cdot}L^{-1}$). After 4 weeks of culture, the highest frequency (83.3%) of shoot formation from sucker explants was obtained from the full MS medium with $1.0mg{\cdot}L^{-1}$ BA. The highest shoot number (3.7) per explant was obtained from the full MS medium with $0.5mg{\cdot}L^{-1}$ BA. After 12 weeks of culture, the number of shoots (15.4) per explant was increased. The highest frequency (95%) of root formation was obtained from the 1/2 MS medium, when the explant with shoot were cultured on the full, 1/2 and 1/4 MS medium. The survival rate of the plantlets after transfer to plastic pots containing sand, soil, and vermiculite (1.1:1, vol.) was 95%. The results indicate that multiple shoot procedure can be applied for an efficient mass propagation of Rubus fruticosus L. ${\times}$ R. parvifolius L.

노지에 있는 교잡종 블랙베리의 근맹아를 채취하여 1.2% sodium hypochlorite 용액에 침지하여 표면 살균한 후 BA를 첨가한 변형된 ${\times}$ 1, ${\times}$ 1/2 MS 고체배지에서 다경유도를 시도하였다. 4주간 배양하였을 때 BA $1mg{\cdot}L^{-1}$가 첨가된 기본 MS배지에서 고빈도(83.3%)의 다경유도가 이루어 졌으며, 절편체당 신초수는 $0.5mg{\cdot}L^{-1}$ BA를 첨가한 MS 기본배지에서 3.7개로 가장 높게 형성되었다. 또한 12주간 배양하였을 때 4주간 한 경우에 비하여 신초형성율이 4.1배 많은 15.4개가 형성됨을 관찰할 수 있었다. 형성된 신초로부터 뿌리발생은 식물생장조절물질이 첨가되지 않은 MS배지를 1/1, 1/2, 1/4배로 희석하여 신초를 배양하였을 때 특히 1/2 MS 배지에서 95%로 가장 양호하였다. 재생된 식물체는 모래 : 원예용상토 : 버미큘라이트(1:1:1, vol.)혼합토양에서 순화하였을 경우 95%의 생존율을 나타내었다.

Keywords

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