생명과학회지 (Journal of Life Science)

  • 제18권4호
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  • Pages.509-514
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  • 2008
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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땅강아지(Gryllotalpa orientalis) 추출물의 항산화 및 항염증 활성

Effects of Mole Crickets (Gryllotalpa orientalis) Extracts on Anti-oxidant and Anti-inflammatory Activities.

  • 허진철 (경북대학교 식품생물산업연구소) ;
  • 이동엽 (경북대학교 생명식품공학부/응용생명과학부) ;
  • 손민식 (경북대학교 생명식품공학부/응용생명과학부) ;
  • 윤치영 (대전대학교 생물학과) ;
  • 황재삼 (농업과학기술원 농업생물부) ;
  • 강석우 (농업과학기술원 농업생물부) ;
  • 김태호 (경북대학교 의과대학 의학과) ;
  • 이상한 (경북대학교 식품생물산업연구소, 경북대학교 생명식품공학부/응용생명과학부)
  • Heo, Jin-Chul (Food and Bio-Industry Research Institute, Kyungpook National University) ;
  • Lee, Dong-Yeob (Department of Life & Food Science, Kyungpook National University) ;
  • Son, Min-Sik (Department of Life & Food Science, Kyungpook National University) ;
  • Yun, Chi-Young (Department of Biology, Daejeon University) ;
  • Hwang, Jae-Sam (National Institute of Agricultural Science & Technology, RDA) ;
  • Kang, Seok-Woo (National Institute of Agricultural Science & Technology, RDA) ;
  • Kim, Tae-Ho (Department of Medicine, Kyungpook National University School of Medicine) ;
  • Lee, Sang-Han (Food and Bio-Industry Research Institute, Kyungpook National University, Department of Life & Food Science, Kyungpook National University)
  • 발행 : 2008.04.30
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초록

동 ${\cdot}$ 식물의 추출물은 예로부터 생약으로 많이 이용이 되었는데, 이는 추출물 내에 다양한 생리 활성 물질 및 화합물을 포함 하고 있기 때문이다. 본 연구는 한방에서 누고(樓姑)라고 알려진 땅강아지 추출물에 대한 항산화 활성을 1,1-diphenyl-2-picrylhydrazyl (DPPH)와 ferric reducing ability of plasma (FRAP)활성 실험을 통하여 알아보았다. 땅강아지는 DW, DMSO, 에탄올, 메탄올에 추출하였다. 실험 결과 땅강아지 추출물의 항산화활성은 DPPH radical 소거 활성의 경우 에탄올 추출물에서 가장 높은 활성을 나타났다. FRAP 활성의 경우 DW 추출물에서 가장 높게 나타났으며, 시간에 따른 활성의 증가는 에탄올 추출물에서 가장 높게 나타났다. Cyclooxygenase-2 promoter의 활성 저해는 DMSO와 에탄올 추출물에서 저해활성이 상대적으로 높은 것으로 미루어 보아서 이들 추출물간의 특성을 잘 응용할 경우 항산화 및 항염증에 좋은 생물학적 재료가 될 것으로 판단된다.

Tremendous natural product extracts were used as a herb medicine remedy or therapy for centuries. Because these extracts have various biological activities, we examined the effects of Gryllotalpa orientalis extract for anti-oxidant and anti-inflammation activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing ability of plasma (FRAP), hydroxy radical scarvenging and cyclooxygenase-2 promoter assays. Gryllotalpa orientalis extracts were prepared from solvents such as distilled water (DW), dimethyl sulfoxide (DMSO), ethanol and methanol. The results showed that Gryllotalpa orientalis extracts have potent DPPH (methanol extract), FRAP (DW extract) and hydroxy radical scavenging (DW and methanol extracts) activity than any other extracts used. A significant inhibition of cyclooxygenase-2 (Cox-2) promoter activity was detected in the presence of DMSO extract or ethanol extract. Collectively, the present results suggested that Gryllotalpa orientalis extract could be used for anti-oxidant and/or anti-inflammation agent for human or agricultural purposes.

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