생명과학회지 (Journal of Life Science)

  • 제18권4호
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  • Pages.503-508
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  • 2008
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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해양심층수의 cytochrome P450 1A1, aromatase 및 MMP-9 활성 억제 효과

Effect of Deep Sea Water on Cytochrome P450 1A1, Aromatase and MMP-9.

  • 손윤희 (동국대학교 지역연고산업진흥사업단, 난치병한양방치료연구소) ;
  • 김미경 (동국대학교 지역연고산업진흥사업단) ;
  • 남경수 (동국대학교 지역연고산업진흥사업단, 동국대학교 의과대학 약리학교실)
  • Shon, Yun-Hee (Regional Innovation System, Intractable Disease Research Center) ;
  • Kim, Mee-Kyung (Regional Innovation System, Dongguk University) ;
  • Nam, Kyung-Soo (Regional Innovation System, Department of Pharmacology, College of Medicine, Dongguk University)
  • 발행 : 2008.04.30
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초록

동해 해양심층수의 유방암예방 효능과 전이에 미치는 영향을 알아보기 위해 cytochrome P450 1A1 활성과 aromatase 활성 및 유방암세포의 침윤성, 이와 관련된 MMP-9 의 활성과 그 단백질 발현에 미치는 영향을 조사하였다. 해양심층수는 체내외의 여러 화학물질을 체내에서 활성화시켜 발암이나 돌연변이 등을 유발시키는 것으로 알려진 cytochrome P450 1A1을 경도의존적으로 저해시켰다. 또한 호르몬 의존성 유방암의 진행에 관여하는 aromatase의 활성도 경도의존적으로 저해시켰다($5.6{\sim}51.9%$). 해양심층수 처리에 의해 사람유방암세포인 MDA-MB-231 세포의 침윤성은 $73.7{\sim}29.4%$로 감소하였으며, 세포의 침윤시 작용하는 단백질 분해 효소인 MMP-9의 활성과 단백질 발현도 경도의존적으로 억제되었다. 따라서 해양심층수는 유방암 예방과 전이관련의 더 많은 연구에 의해 유방암 예방과 전이 억제작용을 증명할 수 있을 것으로 보인다.

Deep sea water from the East sea was tested for breast cancer chemoprevention and metastasis by measuring the activities of cytochrome P450 1A1 and aromatase, invasiveness, and activity and expression of matrix metalloproteinase (MMP)-9 in breast MDA-MB-231 cancer cell. The in vitro incubation of rat liver microsome with deep sea water (a hardness range of $100{\sim}1,000$) showed a hardness-dependent inhibition of 7,12-dimethylbenz[a]anthracene (DMBA)-induced cytochrome P450 1A1 activity. Deep sea water showed 27.1, 45.4 and 51.9% inhibition of microsomal aromatase activity at the hardness of 600, 800 and 1,000, respectively. In addition deep sea water inhibited not only the invasiveness of 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated MDA-MB-231 cells through matrigel-coated membrane in a hardness-dependent manner but also the activity and expression of MMP-9 in MDA-MB-231 cell.

키워드

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피인용 문헌

  1. Suppression of cancer progression and metastasis in HT-29 human colorectal adenocarcinomas by deep sea water vol.18, pp.1, 2013, https://doi.org/10.1007/s12257-012-0543-y
  2. Mineral-enriched deep-sea water inhibits the metastatic potential of human breast cancer cell lines vol.43, pp.5, 2013, https://doi.org/10.3892/ijo.2013.2089